Microbial dynamics during various activities in residential areas of Lahore, Pakistan

Bioaerosols are ubiquitous in the atmosphere with their levels affected by a variety of environmental factors as well as type of activities being carried out at any specific time. The present study investigated how indoor activities influence bioaerosol concentrations in five residential houses of Lahore. Agar coated petri plates were exposed face upwards for twenty minutes in kitchens and living rooms during activity and non-activity periods. The temperature and relative humidity levels were noted as well. The bioaerosol concentrations in kitchens during the activity time ranged between 1022 to 4481 cfu/m3 and in living rooms from 1179 to 3183 cfu/m3 . Lower values were observed during non-activity periods. A paired-t test revealed a significant difference in bacterial loads during activity and non-activity times in both micro-environments (p = 0.038 in kitchen and p = 0.021 in living room). The predominant species identified were Micrococcus spp., Staphylococcus spp., and Bacillus spp. which are a common constituent of the indoor environment and are known to be opportunistic pathogens as well

Evaluation of Records of Oral and Maxillofacial Surgery Cases Reported at Abbasi Shaheed Hospital and Karachi Medical and Dental College, Pakistan

Background: Oral and Maxillofacial Surgery department is a diverse field in dentistry. Record maintenance has been established as one of the key factors in the success and integrity of health care institutes.Objective: The objective of the study was to evaluate the records of oral and maxillofacial surgery casesreported to oral and maxillofacial surgery department, Abbasi Shaheed Hospital and oral surgery OPD ofKarachi Medical and Dental College.Methods: Cross sectional study was conducted in at ASH and KMDC from July 2019 to September 2019.The data from January 2017 to July 2019 was retrospectively noted through electronic surgical recordof ASH and records of the Oral Surgery OPD of KMDC. Inclusion criteria was patients records of bothgenders of 5–70 years age, having complaint of any oral or dental pathology or pathologies, trauma andimpactions. Data was calculated manually by calculating frequencies and percentages for the trauma,impaction and pathology cases of patients.Results: In 2017, 239 cases were treated under general anesthesia from which trauma 11. 45% (n=11),followed by 48. 11% (n=115) cases of oral pathologies, total 11.7% (n=28) cases of complicated exodontias. In2018, among 211, 51.1% (n=108) cases were trauma followed by 39.3% (n=83) cases of oral pathologies,whereas, total 9.4% (n=20) complicated exodontias cases were observed. During 2019 (January to July),168 cases 36.2% (n=62) cases were diagnosed as trauma, in oral pathology, overall 36.2% (n=62) caseswere surgically excised. Total 23.2% (n=39) complicated exodontias. In 2017, 25122 cases were reported in Surgery OPD of Karachi Medical and Dental College. Total 36.2% (n=9097) teeth were extracted from which 1.93% (n=486) cases were surgical impaction. On the other hand, 1.65% (n=416) patients were treated through minor oral surgeries. In 2018, 29008 cases were reported in Surgery OPD. Total 42.7% (n=12377) teeth were extracted from which 0.92% (n=268) cases were surgical impaction. On the other hand, 0.71% (n=208) patients were treated through minor surgeries. In 2019, January till July 13028 cases were reported in Surgery OPD. Total42.6% (n=5559) teeth were extracted from which 0.66% (n=87) cases were surgical impaction. On the other hand, 0.68% (n=89) patients were treated through minor surgeries.Conclusion: It has been concluded that evaluation of the records of oral and maxillofacial surgery casesreported to oral and maxillofacial surgery department, Abbasi Shaheed Hospital and oral surgery OPD ofKarachi Medical and Dental College were high and appropriate measures should be taken in order tomanage these problems timely and effectively

Ultrafine particles in rural and urban dwellings with different household fuel use in developing countries – An example from Pakistan

Exposure to indoor particulate matter (PM) is a major public health concern, in particular, in developing countries where solid fuels are typically used as a household energy source.Despite the fact that emission from these fuels can have a dominant fraction of ultrafine particles, exposure to PM is generally characterised in terms of mass concentration of PM10 and PM2.5. The present study was carried out to examine the number concentration of ultrafine particles in rural and urban Pakistani households with different fuels. Air samples were collected from kitchens, living rooms and courtyards of two rural sites (Site I - Solid fuel; Site II - Natural gas) and an urban site (Natural gas) by using condensation particle counters.At rural site –I the 24 hour mean concentration of particles in the kitchen, living room and outdoors was 40,991#/cm3 (± 7472), 30,291#/cm3 (± 13774) and 34,534#/cm3 (± 4947), respectively. During cooking the number concentration can increase significantly with an average hourly maximum value of 169,455#/cm3. Higher outdoors levels than in living rooms highlight the effect of cooking in open kitchens on ambient levels. At the rural site II the daily average number concentration in living rooms was in the range of 10,745 – 16,126 #/cm3 with a mean of 13,542 #/cm3.These values were more than half those in living rooms at rural site I. Whereas in the kitchen the 24hour mean was 27,446#/cm3 (± 4487). At the urban site the mean 24 hour average in the living rooms and kitchens was 45,466 #/cm3 (± 5919)and 65,904 #/cm3 (± 11490), respectively. The 24 hour mean concentration was more than double in the urban kitchens than in rural kitchens at site II. The 24 hour average outdoors was 33,424 #/cm3 (± 6037)– slightly lower than outdoors at rural site I. Overall, the number concentration was higher in kitchens using natural gas fuel at the urban site than in those with solid fuels and natural gas at rural sites. While between rural sites the households with solid fuel had higher concentrations than those with natural gas. Furthermore, outdoors at rural site-I households had higher concentrations as compared to urban household outdoors

Heavy metal composition of particulate matter in rural and urban residential built environments in Pakistan

Heavy metals in outdoor and indoor airborne particulate matter (PM) and dust in different residential built environmentsat two rural and one urban site in Pakistan were analysed. An eight stage non-viable impactor (Thermo Fisher Scientific Inc., USA) loaded with EMP 2000 glass microfiber filter papers (Whatman, England) was used to collect airborne PM.The indoordust samples (settled dust) were collected from different indoor surfaces (floor, cupboards) in living rooms and kitchens from houses at rural sites. The outdoor samples were collected from courtyards of the houses.At the urban site dust samples were also collected by the roads at 27 different locations around Lahoreand at a background site (University of Veterinary and Animal Sciences).Additionally, samples of dung cake, used as solid fuel, at one of the rural sites were taken.Heavy metals (Si, Al, Zn, Mn, Cu, Ni, Cd, Pb, Co and As) were determined by Graphite Furnace Atomic Absorption Spectrophotometer. At rural site I, in general, the concentrations of metals were higher outdoors than indoors, except for slightly higher indoor levels of Cu (0.85μg/m3 indoor: 0.56 outdoorμg/m3), Si (3.31μg/m3 indoor: 3.17 outdoorμg/m3) and Pb(11.99 ng/m3 indoor: 9.32 outdoor ng/m3). At the rural site II the mean concentration were higher outdoors than indoors, excluding Ni which was considerably higher indoors (55.68 ng/m3) than outdoors (31.91 ng/m3).At the urban site,outdoors, Si had the highest concentration (3.46 μg/m3) followed by Al, Zn, Mn, Cu, Ni, Cd, Pb and Co. Similarly, the indoor levels had a maximum contribution from Si (12.30 μg/m3) followed by Al, Mn, Zn, Cu, Cd, As, Pb, Ni and Co.With reference to dust at rural site I the top five metals outdoors were Si (708 mg/kg), Al, Cu, Zn and Pb (52 mg/kg) while, indoors Al was highest (281 mg/kg), followed by Si, Cu, Zn and Pb (57 mg/kg). At rural site II, both outdoors and indoors, Al (274 mg/kg – outdoor: 266 mg/kg - indoor), Si, Zn, Cu and Pb (61 mg/kg – outdoor: 80 mg/kg - indoor) were the five most abundant metals.The main five metals in decreasing order of their concentration in the road dust around Lahore were Si (686 mg/kg), Al, Cu, Zn and Pb (81 mg/kg). On the other hand, the dust samples from the background site showed Si (345 mg/kg) > Al >Pb> Cu > Zn (73 mg/kg). The airborne metal concentration of Pb was within the guideline value of WHO (0.5 μg/m3) but the levels of Mn, Cd and Ni were higher at all sites than the guidelines proposed by European Commission and WHO highlighting the risk of exposure to toxic metals in non-occupational environments

Automotive related exposure to particulate air pollution in developing countries cities

Poor urban air quality in developing countriesis a growing public health challenge due to rises in population, industries, urbanization and vehiclesalong with insufficient air quality management. Among the range of air pollutants exposure to particulate matter (PM) is of greatest concern due to its association with chronic obstructive pulmonary diseases. The present study reports traffic related exposure to PM by the roads in Lahore, Pakistan. The measurements of mass and number of PM were carried out by GRIMM analysers (Model 1.108 and Model 1.101) and condensation particle counter (TSI 3781). The heavy metals concentration in PM was determined by Graphite Furnace Atomic Absorption Spectrophotometer (Unicam atomic absorption, Cambridge, UK).The mean hourly average concentration of PM10, PM2.5, PM1 and PM10 – 2.5 at the road siteswas higher during weekdays(305 μg/m3, 84 μg/m3, 61 μg/m3 and 222 μg/m3, respectively) in comparison to the weekend (136 μg/m3, 60 μg/m3, 40 μg/m3 and 76μg/m3, respectively). At the background site the levels in the same size fractions were 206μg/m3, 63μg/m3, 31μg/m3, and 143μg/m3, respectively. Likewise, the number concentration of ultrafine particles was considerably higher at road sites (417,003 #/m3) than the background(97,300 #/m3).The concentration of heavy metals in PM decreased in the following order: Si, Al, Zn, Mn, Cu, Ni, Cd, Pb. Overall, the concentration of PM10, PM2.5 and toxic metals (Mn, Cd, Ni) was substantially higher than guidelines by the WHO. Furthermore, relatively higher levels of the fine fraction (PM2.5 and PM1) in the background reflect their higher residence time and resultant increased risk of exposure to the wider public beyond that of the vicinity to automotive sources. Everyday commuters, mostly on two and three wheelers as well asthe residential population in urban areas are at an enhanced risk of exposure to high levels of particulate pollution

Saudi SCD patients’ symptoms and quality of life relative to the number of ED visits

Background Individuals living with sickle cell disease (SCD) have significantly increased emergency department (ED) use compared to the general population. In Saudi Arabia, health care is free for all individuals and therefore has no bearing on increased ED visits. However, little is known about the relationship between quality of life (QoL) and frequency of acute care utilization in this patient population. Methods A cross-sectional study was conducted on 366 patients with SCD who attended the outpatient department at King Fahad Hospital, Hofuf, Saudi Arabia. Data were collected through self-administered surveys, which included: demographics, SCD-related ED visits, clinical issues, and QoL levels. We assessed the ED use by asking for the number of SCD-related ED visits within a 6-month period. Results The self-report survey of ED visits was completed by 308 SCD patients. The median number of SCD-related ED visits within a 6-month time period (IQR) was four (2-7 visits). According to the unadjusted negative binomial model, the rate of SCD-related ED visits increased by (46, 39.3, 40, and 53.5 %) for patients with fever, skin redness with itching, swelling, and blood transfusion, respectively. Poor QoL tends to increase the rate of SCD-related ED visits. Well education and poor general health positively influenced the rate of SCD-related ED visits. Well education tends to increase the rate of SCD-related ED visits by 50.2 %. The rate of SCD-related ED visits decreased by 1.4 % for every point increase in general health. Conclusion Saudi patients with sickle cell disease reported a wide range of SCD-related ED visits. It was estimated that six of 10 SCD patients had at least three ED visits within a 6-month period. Well education and poor general health resulted in an increase in the rate of SCD-related ED visits

Measuring the Awareness of University Students Towards Environmental Pollution: A Case Study of Khorfakkan University Students

The study aimed to measure the towards the environment pollution among university students, and in this a measure was prepared that contains students knowledge of environmental pollution and its effects, and students participation in facing environmental problems. The study relied on the sample social survey method by designing a sample representative of the study community consisting of 300 students at the University of Khorfakkan. A scale was designed to measure students awareness of the environment. To design the scale, a guiding program was relied upon to determine measurement criteria in modifying students behavior towards the environment. This program is composed of reinforcement strategies for self-motivation, education, change and support of positive attitudes, participation, persuasion and knowledge building. The study achieved several results, including a high rate of knowledge among university students, and students awareness of the effects of environmental pollution. On the other hand, there is a decrease in the rate of awareness of environmental issues and the rate of students participation in facing environmental problems

Pseudo-Unitary Operators and Pseudo-Unitary Quantum Dynamics

We consider pseudo-unitary quantum systems and discuss various properties of pseudo-unitary operators. In particular we prove a characterization theorem for block-diagonalizable pseudo-unitary operators with finite-dimensional diagonal blocks. Furthermore, we show that every pseudo-unitary matrix is the exponential of $i=\sqrt{-1}$ times a pseudo-Hermitian matrix, and determine the structure of the Lie groups consisting of pseudo-unitary matrices. In particular, we present a thorough treatment of $2\times 2$ pseudo-unitary matrices and discuss an example of a quantum system with a $2\times 2$ pseudo-unitary dynamical group. As other applications of our general results we give a proof of the spectral theorem for symplectic transformations of classical mechanics, demonstrate the coincidence of the symplectic group $Sp(2n)$ with the real subgroup of a matrix group that is isomorphic to the pseudo-unitary group U(n,n), and elaborate on an approach to second quantization that makes use of the underlying pseudo-unitary dynamical groups.Comment: Revised and expanded version, includes an application to symplectic transformations and groups, accepted for publication in J. Math. Phy

The in-vitro Antibiotic Sensitivity Test of Pasteurella multocida Isolated from Layer and Breeder Chickens

Abstract The current study aimed to characterize Pasteurella multocida (P. multocida) isolates from layers and breeder chickens in Egypt regarding in-vitro antibiotic sensitivity and resistance pattern. In doing so, spleen, liver, lungs, and heart, were taken aseptically from chickens suffering from a drop in egg production, septicemia, respiratory manifestations, and mortalities between 2016 and 2017. To isolate bacteria, samples were grown on a modified Das medium. Moreover, microscopic appearance and biochemical characteristics were used to identify pure colonies of P. multocida isolates. In the next step, in-vitro antibiotic sensitivity testing was performed on the isolated P. multocida. The findings indicated that P. multocida was found in 36 isolates out of 330 investigated chicken flocks. Small glistering, mucoid, grayish, and dew drop P. multocida colonies were discovered during the culture analysis. Pasteurella multocida isolates were Gram-negative coccobacilli using the microscope. Catalase, indole generation, H2S production, nitrate reduction, and oxidase tests were all positive for the sample; however, methyl red, urease activity, Voge's proskaur, and gelatin liquefaction tests were all negative. They also fermented glucose, mannose, fructose, sucrose, mannitol, xylose, and sorbitol without producing gas but not lactose, arabinose, maltose, inositol, salicin, raffinose, or dulcitol. Isolated P. multocida strains were sensitive to tetracycline, erythromycin, trimethoprim/sulphamethoxazole, norfloxacin, ofloxacin, penicillin, chloramphenicol, and azithromycin, while resistant to ampicillin and clindamycin. Cefoperazone, gentamycin, and streptomycin all showed intermediate sensitivity. https://jwps.rovedar.com/index.php/JWPS/article/view/