Manajemen sarana prasarana pendidikan dalam meningkatkan kepuasan civitas akademik MI Roudlotul Huda Lampung Tengah

This study aims to examine the role of management of educational facilities and infrastructure in increasing the satisfaction of madrasah residents in Madrasah Ibtidaiyah Roudlotul Huda Purwosari Padang Ratu, Central Lampung. The research method used is descriptive with data collection techniques through questionnaires and interviews. The respondents in this study were madrasah residents consisting of students, teachers, and madrasah staff. The results showed that the management of educational facilities and infrastructure has an important role in increasing the satisfaction of madrasah residents. The availability of adequate educational facilities and infrastructure such as classrooms, libraries, laboratories, sports facilities, and canteens that are clean and comfortable greatly affects the satisfaction of madrasah residents. In addition, good management in the management of educational facilities and infrastructure such as maintenance, repair, and development of facilities also plays a role in increasing the satisfaction of madrasah residents. In this case, cooperation between madrasah administrators and related parties such as the government and the community is also very important to obtain support in the development of educational facilities and infrastructure. In conclusion, this study shows that the management of educational facilities and infrastructure has a very important role in increasing the satisfaction of madrasah residents. Therefore, efforts need to be made to improve the quality of educational facilities and infrastructure and their management to achieve these goals

Internal control and fraud in construction industry of Malaysia / Wan Noor Asmuni Wan Fauzi,Siti Haliza Asat and Junaidah Hanim Ahmad

The purpose of this paper is to develop an understanding about the internal control and fraud problem in the Malaysian construction industry. It is believed that the construction industry is one of the riskiest industries regarding internal fraud due to its complex and costly nature, and dense third-party contract relationships. If this situation is left untreated, it will lead to more serious problems in the future of construction industry in Malaysia. Therefore, this study is aimed to discuss the fraud phenomenon, lack of internal control and the reasons behind fraud in construction industry. It also proposes that organizations should periodically perform formal risk assessments and internal control reviews to reduce fraud activities. It is recommended to all parties to promote and practice effective internal control in their project to minimize the occurrence of fraud and failures

Employees' pre-dismissal right to be heard: the Malaysian and the Islamic perspective

It seemed to be a settled principle of law that before an employee can be dismissed from his job for misconduct, he must have a notice of the allegation against him and accorded a reasonable opportunity of being heard. However, this principle should be revisited in the light of two conflicting Federal Court’s decisions pertaining to the mandatory issue of this right especially in the private sector employment in Malaysia. The curable principle as enunciated by Dreamland Corporation (M) Sdn. Bhd. v Choong Chin Sooi & Anor [1988] 1 MLJ 111 has watered down the right of the employee to be heard as it was held in that case that the irregularity in holding a domestic enquiry is not fatal to the employer. He may justify the dismissal at the Industrial Court. Despite the existence of this principle, it should be emphasised that the right to a pre-dismissal hearing should be interpreted to be mandatory and not discretionary as there are two statutory provisions and constitutionally recognised rules of natural justice which may support this proposition. The mandatory effect of the domestic enquiry should be consistently upheld. With a view to enhance the employee’s right and to ensure harmonious employer-employee relationship, it would be the purpose of this article to comparatively examine and learn how the same right is treated and regulated under the Islamic principle of justice and its application in two Islamic countries, namely Kingdom of Saudi Arabia and the UAE. Apart from narrowing down the gap in the Islamic knowledge on the administration of justice in dismissal cases, this article may also contribute in idea on how to harmonise the current employment laws and the Sharia

Isolation, purification, and characterization of five active diketopiperazine derivatives from endophytic streptomyces SUK 25 with antimicrobial and cytotoxic activities

In our search for new sources of bioactive secondary metabolites from Streptomyces sp., the ethyl acetate extracts from endophytic Streptomyces SUK 25 afforded five active diketopiperazine (DKP) compounds. The aim of this study was to characterize the bioactive compounds isolated from endophytic Streptomyces SUK 25 and evaluate their bioactivity against multiple drug resistance (MDR) bacteria such as Enterococcus raffinosus, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa, and Enterobacter spp., and their cytotoxic activities against the human hepatoma (HepaRG) cell line. The production of secondary metabolites by this strain was optimized through Thornton's medium. Isolation, purification, and identification of the bioactive compounds were carried out using high-performance liquid chromatography, high-resolution mass liquid chromatography-mass spectrometry, Fourier transform infrared spectroscopy, and nuclear magnetic resonance, and cryopreserved HepaRG cells were selected to test the cytotoxicity. The results showed that endophytic Streptomyces SUK 25 produces four active DKP compounds and an acetamide derivative, which were elucidated as cyclo-(L-Val-L-Pro), cyclo-(L-Leu-L-Pro), cyclo-(L-Phe-L-Pro), cyclo-(L-Val-L-Phe), and N-(7-hydroxy-6-methyl-octyl)-acetamide. These active compounds exhibited activity against methicillin-resistant S. aureus ATCC 43300 and Enterococcus raffinosus, with low toxicity against human hepatoma HepaRG cells. Endophytic Streptomyces SUK 25 has the ability to produce DKP derivatives biologically active against some MDR bacteria with relatively low toxicity against HepaRG cells line

Antibiotic biosynthesis pathways from endophytic streptomyces SUK 48 through metabolomics and genomics approaches

Streptomyces sp. has been known to be a major antibiotic producer since the 1940s. As the number of cases related to resistance pathogens infection increases yearly, discovering the biosynthesis pathways of antibiotic has become important. In this study, we present the streamline of a project report summary; the genome data and metabolome data of newly isolated Streptomyces SUK 48 strain are also analyzed. The antibacterial activity of its crude extract is also determined. To obtain genome data, the genomic DNA of SUK 48 was extracted using a commercial kit (Promega) and sent for sequencing (Pac Biosciences technology platform, Menlo Park, CA, USA). The raw data were assembled and polished using Hierarchical Genome Assembly Process 4.0 (HGAP 4.0). The assembled data were structurally predicted using tRNAscan-SE and rnammer. Then, the data were analyzed using Kyoto Encyclopedia of Genes and Genomes (KEGG) database and antiSMASH analysis. Meanwhile, the metabolite profile of SUK 48 was determined using liquid chromatographymass spectrophotometry (LC-MS) for both negative and positive modes. The results showed that the presence of kanamycin and gentamicin, as well as the other 11 antibiotics. Nevertheless, the biosynthesis pathways of aurantioclavine were also found. The cytotoxicity activity showed IC50 value was at 0.35 � 1.35 mg/mL on the cell viability of HEK 293. In conclusion, Streptomyces sp. SUK 48 has proven to be a non-toxic antibiotic producer such as auranticlavine and gentamicin

Metabolite profiling of endophytic Streptomyces spp. and its antiplasmodial potential

Background: Antiplasmodial drug discovery is significant especially from natural sources such as plant bacteria. This research aimed to determine antiplasmodial metabolites of Streptomyces spp. against Plasmodium falciparum 3D7 by using a metabolomics approach. Methods: Streptomyces strains’ growth curves, namely SUK 12 and SUK 48, were measured and P. falciparum 3D7 IC50 values were calculated. Metabolomics analysis was conducted on both strains’ mid-exponential and stationary phase extracts. Results: The most successful antiplasmodial activity of SUK 12 and SUK 48 extracts shown to be at the stationary phase with IC50 values of 0.8168 ng/mL and 0.1963 ng/mL, respectively. In contrast, the IC50 value of chloroquine diphosphate (CQ) for antiplasmodial activity was 0.2812 ng/mL. The univariate analysis revealed that 854 metabolites and 14, 44 and three metabolites showed significant differences in terms of strain, fermentation phase, and their interactions. Orthogonal partial least square-discriminant analysis and S-loading plot putatively identified pavettine, aurantioclavine, and 4-butyldiphenylmethane as significant outliers from the stationary phase of SUK 48. For potential isolation, metabolomics approach may be used as a preliminary approach to rapidly track and identify the presence of antimalarial metabolites before any isolation and purification can be done

Aktiviti Antiinfektiviti Streptomyces

Buku Aktinobakteria Sumber Antiinfeksi Baharu: Satu Penerokaan ini adalah himpunan hasil kajian sekumpulan penyelidik Kumpulan Penyelidikan Novel Antibiotik UKM berkenaan pencarian sumber antibiotik baharu daripada bakteria endofit yang dipencilkan daripada tumbuhan perubatan tradisional serta tumbuhan semula jadi. Kesedaran terhadap keperluan dadah baharu yang spesifik serta lebih selamat memberi satu motivasi yang besar kepada kumpulan penyelidikan ini untuk meneroka dalam pencarian metabolit sekunder yang aktif daripada sumber semula jadi. Aktinobakteria merupakan bakteria endofit yang kebiasaannya dijumpai pada tisu-tisu tumbuhan seperti akar, buah, daun dan batang tumbuhan yang mempunyai nilai perubatan (etnobotanikal). Kebolehan kumpulan penyelidikan ini di dalam penerokaan pencariaan bakteria endofit yang berupaya menghasilkan sebatian metabolit sekunder semula jadi yang berpotensi sebagai agen antiinfeksi merupakan hasil daripada penyelidikan hampir 10 tahun. Kejayaan ini n secara langsung dapat mengurangkan kebergantungan manusia terhadap dadah sintetik yang berkemungkinan besar boleh mendatangkan kesan sampingan kepada pengguna. Projek penyelidikan ini adalah dibawah tajaan dan naungan beberapa geran penyelidikan iaitu Geran Universiti Penyelidikan (GUP), FRGS dan juga ERGS. Antara lain, keistimewaan buku ini adalah ia boleh dijadikan rujukan kepada individu yang ingin mendalami dan memahami kebaikan bakteria endofit ini dalam menghasilkan dadah alternatif baru yang lebih selamat untuk digunakan

Employees' pre-dismissal right to be heard: The Malaysian and the Islamic perspective

It seemed to be a settled principle of law that before an employee can be dismissed from his job for misconduct, he must have a notice of the allegation against him and accorded a reasonable opportunity of being heard. However, this principle should be revisited in the light of two conflicting Federal Court's decisions pertaining to the mandatory issue of this right especially in the private sector employment in Malaysia. The curable principle as enunciated by Dreamland Corporation (M) Sdn. Bhd. v Choong Chin Sooi & Anor [1988] 1 MLJ 111has watered down the right of the employee to be heard as it was held in that case that the irregularity in holding a domestic enquiry is not fatal to the employer. He may justify the dismissal at the Industrial Court. Despite the existence of this principle, it should be emphasised that the right to a pre-dismissal hearing should be interpreted to be mandatory and not discretionary as there are two statutory provisions and constitutionally recognised rules of natural justice which may support this proposition. The mandatory effect of the domestic enquiry should be consistently upheld. With a view to enhance the employee's right and to ensure harmonious employer-employee relationship, it would be the purpose of this article to comparatively examine and learn how the same right is treated and regulated under the Islamic principle of justice and its application in two Islamic countries, namely Kingdom of Saudi Arabia and the UAE. Apart from narrowing down the gap in the Islamic knowledge on the administration of justice in dismissal cases, this article may also contribute in idea on how to harmonise the current employment laws and the Sharia

Discovery of antimalarial drugs from streptomycetes metabolites using a metabolomic approach

Natural products continue to play an important role as a source of biologically active substances for the development of new drug. Streptomyces, Gram-positive bacteria which are widely distributed in nature, are one of the most popular sources of natural antibiotics. Recently, by using a bioassay-guided fractionation, an antimalarial compound, Gancidin-W, has been discovered from these bacteria. However, this classical method in identifying potentially novel bioactive compounds from the natural products requires considerable effort and is a time-consuming process. Metabolomics is an emerging “omics” technology in systems biology study which integrated in process of discovering drug from natural products. Metabolomics approach in finding novel therapeutics agent for malaria offers dereplication step in screening phase to shorten the process. The highly sensitive instruments, such as Liquid Chromatography-Mass Spectrophotometry (LC-MS), Gas Chromatography-Mass Spectrophotometry (GC-MS), and Nuclear Magnetic Resonance (1 H-NMR) spectroscopy, provide a wide range of information in the identification of potentially bioactive compounds. The current paper reviews concepts of metabolomics and its application in drug discovery of malaria treatment as well as assessing the antimalarial activity from natural products. Metabolomics approach in malaria drug discovery is still new and needs to be initiated, especially for drug research in Malaysia

A conceptual business model of e-fashion: harnessing on big data and analytics

The purpose of this paper is to purpose big data CRM to all online fashion market. This idea has the potential to enhance the system at managing customer data and also the process of order. An ordered set of option for customer to buy online. Desire factors like muslimah modern style where found to have impact on the ranking of the factors. The findings are expected to provide useful guidelines to managers while developing advanced system for customer use. Big data is the large amounts of digital data generated continuously by the population of the global that internal several mostly cannot handle the scale of the information. Big data analytics is the process of examining large amount of data. Big data is characterized by dimension of volume, variety, and velocity. There is some well-established data such as big data CRM (Big Data Customer Relationship Management). A mixed method has been used for the research. Examples from secondary sources have been drawn as a part of the literature review which contain about the importance of fashion. Benchmarking to improve the quality of the business which is compared with other e-commerce and successful fashion online