Nonequilibrium Stefan-Boltzmann law

We study thermal radiation outside equilibrium. The situation considered consists of two bodies emitting photons at two different temperatures. We show that the system evolves to a stationary state characterized by an energy current which satisfies a Stefan-Boltzmann-like law expressing it as the difference of the temperatures to the fourth power of the emitters . The results obtained show how the classical laws governing the thermal radiation at equlibrium can be generalized away from equilibrium situations.Comment: 9 pages, 1 figure. To be published in J. Noneq. Ther

Dynamin- and Rab5-Dependent Endocytosis of a Ca²⁺-Activated K⁺ Channel, KCa2.3

Regulation of the number of ion channels at the plasma membrane is a critical component of the physiological response. We recently demonstrated that the Ca2+-activated K+ channel, KCa2.3 is rapidly endocytosed and enters a Rab35- and EPI64C-dependent recycling compartment. Herein, we addressed the early endocytic steps of KCa2.3 using a combination of fluorescence and biotinylation techniques. We demonstrate that KCa2.3 is localized to caveolin-rich domains of the plasma membrane using fluorescence co-localization, transmission electron microscopy and co-immunoprecipitation (co-IP). Further, in cells lacking caveolin-1, we observed an accumulation of KCa2.3 at the plasma membrane as well as a decreased rate of endocytosis, as assessed by biotinylation. We also demonstrate that KCa2.3 and dynamin II are co-localized following endocytosis as well as demonstrating they are associated by co-IP. Further, expression of K44A dynamin II resulted in a 2-fold increase in plasma membrane KCa2.3 as well as a 3-fold inhibition of endocytosis. Finally, we evaluated the role of Rab5 in the endocytosis of KCa2.3. We demonstrate that expression of a dominant active Rab5 (Q79L) results in the accumulation of newly endocytosed KCa2.3 on to the membrane of the Rab5-induced vacuoles. We confirmed this co-localization by co-IP; demonstrating that KCa2.3 and Rab5 are associated. As expected, if Rab5 is required for the endocytosis of KCa2.3, expression of a dominant negative Rab5 (S34N) resulted in an approximate 2-fold accumulation of KCa2.3 at the plasma membrane. This was confirmed by siRNA-mediated knockdown of Rab5. Expression of the dominant negative Rab5 also resulted in a decreased rate of KCa2.3 endocytosis. These results demonstrate that KCa2.3 is localized to a caveolin-rich domain within the plasma membrane and is endocytosed in a dynamin- and Rab5-dependent manner prior to entering the Rab35/EPI64C recycling compartment and returning to the plasma membrane. © 2012 Gao et al

Masa Kerja, Sikap Kerja dan Kejadian Sindrom Karpal pada Pembatik

Sikap kerja yang tidak alamiah dan cara kerja yang tidak ergonomis dalam waktu lama dapat menyebabkan berbagai gangguan kesehatan pada pekerja salah satunya yaitu gangguan gerakan pada bagian tubuh tertentu seperti tangan atau disebut musculoskeletal, salah satunya sindrom terowongan karpal. Pembatik merupakan pekerja sektor informal, dimana pada saat membatik melakukan gerakan berulang, gerakan tangan dengan kekuatan, posisi fleksi dan ekstensi, sehingga menyebabkan stress pada jaringan disekitar terowongan karpal. Tujuan penelitian ini untuk mengetahui hubungan masa kerja dan sikap kerja dengan kejadian STK pada pembatik CV. Pusaka Beruang. Penelitian dilakukan pada tahun 2011, dengan menggunakan pendekatan cross sectional. Populasi dalam penelitian berjumlah 68 orang, dengan sampel berjumlah 22 responden. Instrument penelitian berupa kuesioner dan pengukuran tes phalen. Data dianalisis dengan uji Chi-square. Hasil penelitian menunjukkan bahwa ada hubungan masa kerja p=0,029 dan sikap kerja p=0,031 dengan kejadian STK. Masa kerja > 4 tahun dan level sikap kerja yang tinggi mempunyai risiko STK karena terjadi stress disekitar jaringan terowongan karpal. Simpulan pada penelitian ini ada hubungan antara masa kerja dan sikap kerja dengan kejadian STK.Work attitudes and ways of working that are not ergonomic for a long time can cause a variety of health problems in workers one of the disruption movement on certain body parts hand or musculoskeletal, are one of the sindrom terowongan karpal Batik is an informal sector workers describe or designed batik with the traditional way of working. Which time both do repetitive motion, hand movements with strength, position, position the extension and flexion, hand static, thus causing stress on the network around the carpal tunnel. The purpose of this research is to know the relation tenure and working attitude with STK batik meker in CV. Pusaka Beruang. This research uses the approach of cross sectional. The population in this research is totalling 68 peoples. Sample research amounted to 22 respondents. Research Instrument in the form of questionnaires and measurement test phalen. Data were analyzed by Chi square test. The results showed that there was a relationship tenure p = 0.029 and working attitude p = 0.031 with STK events. Tenure > 4 years and a high level of work attitudes have STK risk due to stress around the carpal tunnel syndrome network. The conclusions in this study no relationship tenure and work attitude with STK

Pengaruh Meal Experience Terhadap Kepuasan Konsumen Di Gusto Gelato & Caffé Bali: Analisa Five Aspects Meal Model

: The objective of this research is to find out the effects of Five Aspects Meal Model on Consumer satisfaction in Gusto Gelato & Caffé. Analysis technique used in this research is descriptive statistical analysis with research tool in the form of SPSS. The respondents are 432 consumer at Gusto Gelato & Caffé. The result of this research proves that room, product dan atmosphere has a positive and significant impact on Consumer satisfaction at Gusto Gelato & Caffé

Laser-plasma interactions with a Fourier-Bessel Particle-in-Cell method

A new spectral particle-in-cell (PIC) method for plasma modeling is presented and discussed. In the proposed scheme, the Fourier-Bessel transform is used to translate the Maxwell equations to the quasi-cylindrical spectral domain. In this domain, the equations are solved analytically in time, and the spatial derivatives are approximated with high accuracy. In contrast to the finite-difference time domain (FDTD) methods that are commonly used in PIC, the developed method does not produce numerical dispersion, and does not involve grid staggering for the electric and magnetic fields. These features are especially valuable in modeling the wakefield acceleration of particles in plasmas. The proposed algorithm is implemented in the code PLARES-PIC, and the test simulations of laser plasma interactions are compared to the ones done with the quasi-cylindrical FDTD PIC code CALDER-CIRC.Comment: submitted to Phys. Plasma

Uji Viabilitas Bakteri Asam Laktat dari Usus Itik pada Media Pakan Dedak Padi dan Kombinasi Dedak Padi dengan Molases

Penelitian ini bertujuan untuk mengetahui viabilitas Bakteri Asam Laktat dari usus itik pada dedak padi dan kombinasi dedak padi dengan molases sebagai kandidat probiotik. Penelitian dilaksanakan dalam Percobaan Rancangan Acak Kelompok yang dilakukan secara faktorial 2 x 6. Faktor A adalah 2 macam media perlakuan yaitu: media A (dedak padi) dan media B (dedak padi + molasis). Faktor B adalah lama waktu inkubasi yaitu 0 jam (kontrol), 2jam, 4jam, 6 jam, 8 jam, dan 10 jam. Masing- masing perlakuan diulang sebanyak 2 kali. Pengamatan viabilitas bakteri dilakukan dengan metode angka lempeng total untuk menentukan jumlah sel bakteri yang hidup dilanjutkan dengan menghitung viabilitas BAL. Data jumlah koloni BAL yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan isolat BAL dari usus itik pada media perlakuan dedak padi dan kombinasi dedak padi dengan molases memiliki viabilitas tertinggi pada waktu inkubasi ke- 10 jam dengan jumlah populasi sebesar Log 6,53 CFU/g pada dedak padi dan Log 6,87 CFU/g pada kombinasi dedak padi dan molases

Investigating the functionality of an OCT4-short response element in human induced pluripotent stem cells.

Pluripotent stem cells offer great therapeutic promise for personalized treatment platforms for numerous injuries, disorders, and diseases. Octamer-binding transcription factor 4 (OCT4) is a key regulatory gene maintaining pluripotency and self-renewal of mammalian cells. With site-specific integration for gene correction in cellular therapeutics, use of the OCT4 promoter may have advantages when expressing a suicide gene if pluripotency remains. However, the human OCT4 promoter region is 4 kb in size, limiting the capacity of therapeutic genes and other regulatory components for viral vectors, and decreasing the efficiency of homologous recombination. The purpose of this investigation was to characterize the functionality of a novel 967bp OCT4-short response element during pluripotency and to examine the OCT4 titer-dependent response during differentiation to human derivatives not expressing OCT4. Our findings demonstrate that the OCT4-short response element is active in pluripotency and this activity is in high correlation with transgene expression in vitro, and the OCT4-short response element is inactivated when pluripotent cells differentiate. These studies demonstrate that this shortened OCT4 regulatory element is functional and may be useful as part of an optimized safety component in a site-specific gene transferring system that could be used as an efficient and clinically applicable safety platform for gene transfer in cellular therapeutics

Kepuasan Pasien Pengguna Kartu Jakarta Sehat di Unit Rawat Jalan Rumah Sakit

Peluncuran Kartu Jakarta Sehat (KJS) pada bulan November 2012 mengakibatkan jumlah kunjungan pasien yang melonjak drastis di fasilitas kesehatan, khususnya rumah sakit, baik itu rumah sakit pemerintah pusat, rumah sakit pemerintahdaerah maupun rumah sakit swasta yang berkomitmen dengan Pemerintah Daerah Jakarta. KJS memberikan kemudahan akses layanan kesehatan agar pasien dapat berobat secara gratis. Namun persoalan yang muncul kemudian adalah pasien harus mengantre lama di loket pendaftaran, ruang pemeriksaan, maupun ruang pengambilan obat. Penelitian ini bertujuan untuk mengetahui hubungan faktor sosial budaya, pribadi, dan psikologis dengan kepuasan pasien pengguna KJS pada 110 responden di Unit Rawat Jalan RSU UKI, dan telah dilaksanakan pada bulan Maret 2013. Rancangan penelitian yang digunakan adalah deskriptif kuantitatif dengan pendekatan potong lintang. Hasil penelitian menunjukkan kepuasan pasien KJS sebesar 40,9%. Akses ke rumah sakit, motivasi, informasi baik dan informasi tidak baik berhubungan signifikan dengan kepuasan pasien KJS, tetapi motivasi adalah hal yang paling dominan berhubungan. Selain itu, kepuasan pasien KJS juga berhubungan bermakna dengan pemanfaatan kembali layanan dan minat merekomendasikan layanan pada orang lain.Jakarta Health Card (JHC) was launched on November 2012, followed by sudden increase number of patient in any hospital accordingly. Jakarta Health Card ease the patient to afford health care without payment. We conducted study on the relation among socio-culture, demographic characteristics, and psychology factors toward patient satisfaction in using JHC. The study subjects were 110 outpatient of UKI Hospital, with study design descriptive quantitative with cross sectional approach. The result showed 40,9% of the patients were satisfied with the hospital service. Factors such as hospital access, motivation, good information and bad information were associated with patient satisfaction significantly, and motivation was the dominant factor. Besides, patient satisfaction also has significant correlation with revisiting/ reusing and recommendation of the hospital to other patients