5 research outputs found
New insights into molecular pathways associated with flatfish ovarian development and atresia revealed by transcriptional analysis
<p>Abstract</p> <p>Background</p> <p>The Senegalese sole (<it>Solea senegalensis</it>) is a marine flatfish of increasing commercial interest. However, the reproduction of this species in captivity is not yet controlled mainly because of the poor knowledge on its reproductive physiology, as it occurs for other non-salmonid marine teleosts that exhibit group-synchronous ovarian follicle development. In order to investigate intra-ovarian molecular mechanisms in Senegalese sole, the aim of the present study was to identify differentially expressed genes in the ovary during oocyte growth (vitellogenesis), maturation and ovarian follicle atresia using a recently developed oligonucleotide microarray.</p> <p>Results</p> <p>Microarray analysis led to the identification of 118 differentially expressed transcripts, of which 20 and 8 were monitored by real-time PCR and in situ hybridization, respectively. During vitellogenesis, many up-regulated ovarian transcripts had putative mitochondrial function/location suggesting high energy production (NADH dehydrogenase subunits, cytochromes) and increased antioxidant protection (selenoprotein W2a), whereas other regulated transcripts were related to cytoskeleton and zona radiata organization (zona glycoprotein 3, alpha and beta actin, keratin 8), intracellular signalling pathways (heat shock protein 90, Ras homolog member G), cell-to-cell and cell-to-matrix interactions (beta 1 integrin, thrombospondin 4b), and the maternal RNA pool (transducer of ERBB2 1a, neurexin 1a). Transcripts up-regulated in the ovary during oocyte maturation included ion transporters (Na<sup>+</sup>-K<sup>+</sup>-ATPase subunits), probably required for oocyte hydration, as well as a proteinase inhibitor (alpha-2-macroglobulin) and a vesicle calcium sensor protein (extended synaptotagmin-2-A). During follicular atresia, few transcripts were found to be up-regulated, but remarkably most of them were localized in follicular cells of atretic follicles, and they had inferred roles in lipid transport (apolipoprotein C-I), chemotaxis (leukocyte cell-derived chemotaxin 2,), angiogenesis (thrombospondin), and prevention of apoptosis (S100a10 calcium binding protein).</p> <p>Conclusion</p> <p>This study has identified a number of differentially expressed genes in the ovary that were not previously found to be regulated during ovarian development in marine fish. Specifically, we found evidence, for the first time in teleosts, of the activation of chemoattractant, angiogenic and antiapoptotic pathways in hypertrophied follicular cells at the onset of ovarian atresia.</p
Geochemical and metallogenetical study of the pegmatites and associated granites from southern Comechingones pegmatitic field, Córdoba
El distrito pegmatítico Comechingones, ubicado en el faldeo oriental de la sierra homónima, en la provincia de Córdoba, involucra pegmatitas graníticas correspondientes a la clase de Elementos Raros, tipo berilo, subtipo berilo-columbita-fosfatos, algunas en transición a la clase muscovítica, con mineralizaciones de Be-Nb- Ta-U y minerales industriales. Dos tipos de pegmatitas graníticas han sido descriptas en el sector sur del distrito:
pegmatitas tipo I, con tamaños que en total pueden alcanzan los 1000 metros de longitud y superar los 50 de ancho, internamente zonadas y portadoras de Be, Nb-Ta y U; y pegmatitas tipo II, de menores dimensiones, no zonadas, ricas en cuarzo de alta pureza, carentes de mineralizaciones metalíferas, y asociadas espacial y genéticamente con leucogranitos aplíticos. En este trabajo se presentan y discuten los datos geoquímicos preliminares de ambos tipos de pegmatitas y granitos asociados. Los datos geoquímicos obtenidos, apoyados con descripciones de campo y petrográficas, permiten establecer que las dos tipologías de pegmatitas corresponden a dos eventos magmáticos diferentes (muy probablemente diacrónicos). El primero generó las pegmatitas tipo I, las cuales de sur a norte presentan un aumento en el grado de fraccionamiento desde pegmatitas poco evolucionadas y sin mineralizaciones de elementos raros, hasta pegmatitas evolucionadas con depósitos metalíferos de interés económico. El segundo evento dio origen a las pegmatitas tipo II y a los granitos aplíticos, y carece de especialización metalogenética, evidenciado por los indicadores de diferenciación magmática sistemáticamente inferiores a los de las pegmatitas tipo I y a la carencia de mineralizaciones metalíferas.The Comechingones pegmatitic field (CPF) is located in the eastern flank of the Sierra de Comechingones, Córdoba province. It is composed of granite pegmatites belonging to the Rare-Element class, beryl type, beryl-columbite-phosphate subtype; some of them are transitional into the Muscovite class. Beryllium, Nb, Ta and U deposits, as well as high-quality industrial mineral deposits, are frequently associated with these pegmatites. In the southern part of the CPF two different pegmatite types have been described.
Type I pegmatites constitute large zoned bodies with up to 1000 m long and 50 m thick, and may constitute rare element deposits, whereas type II pegmatites occur as small, unzoned quartz-rich dykes, without metalliferous mineralizations, spatial and genetically associated with aplitic leucogranites. Preliminary geochemical data from both pegmatites types and granites are presented and discussed in this contribution. Geochemical evidences, supported by field and petrographic observations, suggest that the two types of pegmatites identified in the study area represent two different, probably diachronic, magmatic stages. Type I pegmatites display a geochemical gradation in a S-N direction, from barren pegmatites in the south to fractionated pegmatites in the northern part of the study area, and are the lithological product of the first magmatic stage. The second stage lead to the crystallization of aplitic granites and barren type II pegmatites, geochemically less fractionated than type II pegmatites.Centro de Investigaciones Geológica
Cryo-soft X-ray tomography as a quantitative three-dimensional tool to model nanoparticle:cell interaction
Geochemical and metallogenetical study of the pegmatites and associated granites from southern Comechingones pegmatitic field, Córdoba
El distrito pegmatítico Comechingones, ubicado en el faldeo oriental de la sierra homónima, en la provincia de Córdoba, involucra pegmatitas graníticas correspondientes a la clase de Elementos Raros, tipo berilo, subtipo berilo-columbita-fosfatos, algunas en transición a la clase muscovítica, con mineralizaciones de Be-Nb- Ta-U y minerales industriales. Dos tipos de pegmatitas graníticas han sido descriptas en el sector sur del distrito:
pegmatitas tipo I, con tamaños que en total pueden alcanzan los 1000 metros de longitud y superar los 50 de ancho, internamente zonadas y portadoras de Be, Nb-Ta y U; y pegmatitas tipo II, de menores dimensiones, no zonadas, ricas en cuarzo de alta pureza, carentes de mineralizaciones metalíferas, y asociadas espacial y genéticamente con leucogranitos aplíticos. En este trabajo se presentan y discuten los datos geoquímicos preliminares de ambos tipos de pegmatitas y granitos asociados. Los datos geoquímicos obtenidos, apoyados con descripciones de campo y petrográficas, permiten establecer que las dos tipologías de pegmatitas corresponden a dos eventos magmáticos diferentes (muy probablemente diacrónicos). El primero generó las pegmatitas tipo I, las cuales de sur a norte presentan un aumento en el grado de fraccionamiento desde pegmatitas poco evolucionadas y sin mineralizaciones de elementos raros, hasta pegmatitas evolucionadas con depósitos metalíferos de interés económico. El segundo evento dio origen a las pegmatitas tipo II y a los granitos aplíticos, y carece de especialización metalogenética, evidenciado por los indicadores de diferenciación magmática sistemáticamente inferiores a los de las pegmatitas tipo I y a la carencia de mineralizaciones metalíferas.The Comechingones pegmatitic field (CPF) is located in the eastern flank of the Sierra de Comechingones, Córdoba province. It is composed of granite pegmatites belonging to the Rare-Element class, beryl type, beryl-columbite-phosphate subtype; some of them are transitional into the Muscovite class. Beryllium, Nb, Ta and U deposits, as well as high-quality industrial mineral deposits, are frequently associated with these pegmatites. In the southern part of the CPF two different pegmatite types have been described.
Type I pegmatites constitute large zoned bodies with up to 1000 m long and 50 m thick, and may constitute rare element deposits, whereas type II pegmatites occur as small, unzoned quartz-rich dykes, without metalliferous mineralizations, spatial and genetically associated with aplitic leucogranites. Preliminary geochemical data from both pegmatites types and granites are presented and discussed in this contribution. Geochemical evidences, supported by field and petrographic observations, suggest that the two types of pegmatites identified in the study area represent two different, probably diachronic, magmatic stages. Type I pegmatites display a geochemical gradation in a S-N direction, from barren pegmatites in the south to fractionated pegmatites in the northern part of the study area, and are the lithological product of the first magmatic stage. The second stage lead to the crystallization of aplitic granites and barren type II pegmatites, geochemically less fractionated than type II pegmatites.Centro de Investigaciones Geológica
Geoquímica y metalogénesis de las pegmatitas y granitos asociados del sector sur del distrito Comechingones, Córdoba
Geochemical and metallogenetical study of the pegmatites and associated granites from southern Comechingones pegmatitic field, Córdoba. The Comechingones pegmatitic field (CPF) is located in theeastern flank of the Sierra de Comechingones, Córdoba province. It is composed of granite pegmatites belonging to the Rare-Element class, beryl type, beryl-columbite-phosphate subtype; some of them are transitional into the Muscovite class. Beryllium, Nb, Ta and U deposits, as well as high-quality industrial mineral deposits, are frequently associated with these pegmatites. In the southern part of the CPF two different pegmatite types have been described.Type I pegmatites constitute large zoned bodies with up to 1000 m long and 50 m thick, and may constitute rare element deposits, whereas type II pegmatites occur as small, unzoned quartz-rich dykes, without metalliferous mineralizations, spatial and genetically associated with aplitic leucogranites. Preliminary geochemical data from bothpegmatites types and granites are presented and discussed in this contribution. Geochemical evidences, supported by field and petrographic observations, suggest that the two types of pegmatites identified in the study area represent two different, probably diachronic, magmatic stages. Type I pegmatites display a geochemical gradation in a S-N direction, from barren pegmatites in the south to fractionated pegmatites in the northern part of the study area, andare the lithological product of the first magmatic stage. The second stage lead to the crystallization of aplitic granites and barren type II pegmatites, geochemically less fractionated than type II pegmatites