Norms, attitudes and gender perspectives in ecological sanitation

In Kenya, the urban poor are residing in the Peri urban areas of Nakuru Municipality with inadequate sanitation facilities. This study investigated attitudes towards urine diverting toilets to popularize their use as viable alternative sanitation. Face to face interviews among 231 household respondents using a semi structured questionnaire, field observations, focused group discussions and key informant interviews were performed. Most of the respondents reported that the ecosan toilets are a viable option due to congested housing pattern, desire to own a toilet (for those who never had a toilet before), geohydrological conditions, improved privacy, and convenience among other factors. Most respondents preferred squatting type of urine diverting toilet, and are willing to reuse sanitized urine and feacal matter. Among the challenges noted include forgetfulness by some people to spray ash after defecating, misplaced water entering the toilet vault during cleaning and confusion in using the two holes. The provision for squat hole covers; modification of design, commitment and household change of behaviors are fundamental for encouraging more adoption and replication of the toilets. The study recommends more sanitation is dignity awareness campaigns through social marketing of Urine diverting toilets to salvage available water for improved livelihoods

Overview of parametric survival analysis for health-economic applications.

Health economic models rely on data from trials to project the risk of events (e.g., death) over time beyond the span of the available data. Parametric survival analysis methods can be applied to identify an appropriate statistical model for the observed data, which can then be extrapolated to derive a complete time-to-event curve. This paper describes the properties of the most commonly used statistical distributions as a basis for these models and describes an objective process of identifying the most suitable parametric distribution in a given dataset. The approach can be applied with both individual-patient data as well as with survival probabilities derived from published Kaplan-Meier curves. Both are illustrated with analyses of overall survival from the Sorafenib Hepatocellular Carcinoma Assessment Randomised Protocol trial

A VAR Analysis of the Relationship between Energy Consumption and Economic Growth in Nigeria

The indispensible factor for sustainable economic development in every economy is energy. It is an engine room for rapid socio-economic growth and development. In Nigeria, the problem of insufficient electricity supply (power) is as the result of lack of diversification of energy sources. Electricity generation cannot cope with the ever increasing demand for the product. As a result of rapid population growth, access to modern energy remains relatively low and inequitable. However, there is a functional relationship between the quantity of electricity generation (megawatt) and sustainable development in the economy. This paper investigated the direction of this functional relationship between energy and sustainable economic development using the Granger causality test. Also a multiple regression analysis was adopted to explore the impact of energy consumption on economic growth. The result showed that energy consumption had a bidirectional relationship with GDP growth, and directly contributed significantly to economic development in Nigeria. To this end, therefore, there is need for policy makers to boost investment in the energy sector in order to increase its supply, and meeting the demand for energy with a view to meeting the expectations of the millennium development goals. Keywords: sustainable development, energy consumption, energy potentials and economic growt

A European survey on the insights of patients living with metastatic colorectal cancer: the patient journey before, during and after diagnosis - an Eastern European perspective

Background: Despite being highly preventable and treatable if diagnosed early, colorectal cancer (CRC) remains the second leading cause of cancer-related death in Europe. Limited information is available from the patient perspective on the persisting unmet needs of the journey of the patient with CRC. Objective: To capture European metastatic CRC (mCRC) patients' insights during the patient journey (prediagnosis; diagnosis; postdiagnosis) through a patient survey. Methods: In total, 883 patients from 15 European countries participated. Participants were divided into four groups from Hungary, Poland, Serbia and 'other European countries' (n=103, 163, 170 and 447 patients, respectively). Results: General awareness of CRC and its symptoms prediagnosis varied among groups, with patients from Poland recording the lowest levels. Screening practices and attitudes also varied; while more patients from Serbia had been invited to CRC screening (~15%) compared with the other groups, the ones not invited claimed mostly (~20%) that would not have attended if they had been invited. Whereas most patients were diagnosed within a month after the first consultation/positive screening, the percentages varied substantially being lowest among patients in Poland (~30%) and Serbia (~25%). Although CRC-related information provision varied, with most informed patients from Hungary (~90%) and least from Serbia (~50%), all groups requested an easier-to-understand language by the healthcare team. Approximately 50% of patients from Eastern Europe had to wait longer than a month to receive treatment, in contrast to ~30% from other European countries. All groups emphasised the unmet need for support from psychologists and other patients. Conclusions: Our survey reveals the key aspects of the journey of the patient with mCRC and highlights the areas of similarities and differences between patients with mCRC from Eastern Europe versus those from other European countries as well as among patients from different Eastern European countries, calling for improvement particularly around awareness, screening, treatment availability, communication and support networks

Dose-Dependent and Subset-Specific Regulation of Midbrain Dopaminergic Neuron Differentiation by LEF1-Mediated WNT1/b-Catenin Signaling

The mesodiencephalic dopaminergic (mdDA) neurons, including the nigrostriatal subset that preferentially degenerates in Parkinson's Disease (PD), strongly depend on an accurately balanced Wingless-type MMTV integration site family member 1 (WNT1)/beta-catenin signaling pathway during their development. Loss of this pathway abolishes the generation of these neurons, whereas excessive WNT1/b-catenin signaling prevents their correct differentiation. The identity of the cells responding to this pathway in the developing mammalian ventral midbrain (VM) as well as the precise progression of WNT/b-catenin action in these cells are still unknown. We show that strong WNT/b-catenin signaling inhibits the differentiation of WNT/b-catenin-responding mdDA progenitors into PITX3(+) and TH+ mdDA neurons by repressing the Pitx3 gene in mice. This effect is mediated by RSPO2, a WNT/b-catenin agonist, and lymphoid enhancer binding factor 1 (LEF1), an essential nuclear effector of the WNT/b-catenin pathway, via conserved LEF1/T-cell factor binding sites in the Pitx3 promoter. LEF1 expression is restricted to a caudolateral mdDA progenitor subset that preferentially responds to WNT/b-catenin signaling and gives rise to a fraction of all mdDA neurons. Our data indicate that an attenuation of WNT/b-catenin signaling in mdDA progenitors is essential for their correct differentiation into specific mdDA neuron subsets. This is an important consideration for stem cell-based regenerative therapies and in vitro models of neuropsychiatric diseases

Clinical grade ACE2 as a universal agent to block SARS-CoV-2 variants

The recent emergence of multiple SARS-CoV-2 variants has caused considerable concern due to both reduced vaccine efficacy and escape from neutralizing antibody therapeutics. It is, therefore, paramount to develop therapeutic strategies that inhibit all known and future SARS-CoV-2 variants. Here, we report that all SARS-CoV-2 variants analyzed, including variants of concern (VOC) Alpha, Beta, Gamma, Delta, and Omicron, exhibit enhanced binding affinity to clinical grade and phase 2 tested recombinant human soluble ACE2 (APN01). Importantly, soluble ACE2 neutralized infection of VeroE6 cells and human lung epithelial cells by all current VOC strains with markedly enhanced potency when compared to reference SARS-CoV-2 isolates. Effective inhibition of infections with SARS-CoV-2 variants was validated and confirmed in two independent laboratories. These data show that SARS-CoV-2 variants that have emerged around the world, including current VOC and several variants of interest, can be inhibited by soluble ACE2, providing proof of principle of a pan-SARS-CoV-2 therapeutic

‘A victim, and that’s all’: the construction of Meredith Kercher in the British national newspapers

What journalistic techniques are employed to construct a newsworthy female murder victim in the age of social media? Focusing on Meredith Kercher’s murder, this article examines how Kercher’s victim persona was fashioned by the British national press before the arrests of Amanda Knox and two others. Contrary to prevailing perspectives, we argue that the newsworthy murder victim is not necessarily an ideal victim. Murder victim status in a news story is a multi-facetted construction, contingent on whatever will magnify newsworthiness. The Kercher case is the first example of British journalists using the murder victim’s Facebook content as the primary news resource, adding “authenticity” to the text and images used, since it originated from the victim herself. The research findings illustrate how Kercher was sexualized through the application of misogynist victim-blaming templates used in the reporting of sex crimes. It was the degradation of her ideal victim status that drove the initial newsworthiness of the murder story

The human body at cellular resolution: the NIH Human Biomolecular Atlas Program

Abstract: Transformative technologies are enabling the construction of three-dimensional maps of tissues with unprecedented spatial and molecular resolution. Over the next seven years, the NIH Common Fund Human Biomolecular Atlas Program (HuBMAP) intends to develop a widely accessible framework for comprehensively mapping the human body at single-cell resolution by supporting technology development, data acquisition, and detailed spatial mapping. HuBMAP will integrate its efforts with other funding agencies, programs, consortia, and the biomedical research community at large towards the shared vision of a comprehensive, accessible three-dimensional molecular and cellular atlas of the human body, in health and under various disease conditions