Prospects of endosperm DNA in maize seed characterization

DNA based characterisation of maize germplasm has become the easiest and fastest approach to identify genetic diversity as compared to phenotyping. The conventional DNA source for genotyping is the leaf which required at least 2 weeks waiting period from seed planting to leaves sampling. This work exploits the use of endosperm DNA (EDNA) for the genotyping of maize germplasm. Maize endosperm was excised from maize seeds using pli¬ers, ground and used for Genomic DNA extraction (gDNA). Leaves DNA (LDNA) was also extracted concurrently. The extracted LDNA and EDNA were quantified and subjected to SSR-PCR. The mean concentrations of DNA extracted were 1575 ng/ul for the leaves and 526 ng/ul for endosperm. Though the difference in quantity of EDNA and LDNA were highly significant, the quality (A260/A280) for both EDNA and LDNA fall within 1.6-1.8 range of pure DNA index. SSR-PCR products using phi032 were similar for both EDNA and LDNA, indicating the usability of EDNA in genotyping. This seed based method of gDNA extraction takes less than 24 hours from sampling to quantification and genotyping. It also allows germination of sampled seeds, selection before planting, avoids the delay of planting and waiting in leaf sampling and saves field space

Selection Rules for One- and Two-Photon Absorption by Excitons in Carbon Nanotubes

Recent optical absorption/emission experiments showed that the lower energy optical transitions in carbon nanotubes are excitonic in nature, as predicted by theory. These experiments were based on the symmetry aspects of free electron-hole states and bound excitonic states. The present work shows, however, that group theory does not predict the selection rules needed to explain the two photon experiments. We obtain the symmetries and selection rules for the optical transitions of excitons in single-wall carbon nanotubes within the approach of the group of the wavevector, thus providing important information for the interpretation of theoretical and experimental optical spectra of these materials.Comment: 4 pages, 1 figure, 1 tabl

Hydrocarbon degradation by autochthonous species of Bacillus cereus and Pseudomonas aeruginosa Isolated from Kaduna Refinery Effluents

The present work was undertaken to assess the extent of crude oil degradation by Bacillus cereus and Pseudomonas aeruginosa isolated from effluents collected from Kaduna Refining and Petrochemical Company, Kaduna, Nigeria. The samples were analyzed microbiologically using standard microbiological techniques. The isolates of Bacillus cereus and Pseudomonas aeruginosa were studied to determine their biodegrading capacities on varying concentrations of crude oil as the sole carbon source using minimal medium. The bacterial growth (increase in cell number cfu/ml) was used as indices of biodegradation. The test on the degrading activity of isolates on crude oil from effluent samples revealed that Bacillus cereus and Pseudomonas aeruginosa were the potent degraders of crude oil. There was statistically significant association between time and the increase in bacterial cell numbers (P = 0.00 < 0.05). The implication of the study in relation to biodegradative activity has been discussed

Isolation and Characterization of some Hydrocarbon Utilizing bacteria from Refinery Effluents

The present work was undertaken to isolate and characterize the hydrocarbon degrading bacteria associated with effluent samples collected from Kaduna refinery and petrochemical company (KRPC). The samples were analyzed microbiologically using standard microbiological techniques. Bacillus cereus and Pseudomonas aeruginosa were successfully isolated from the refinery effluents. However, following treatment before the effluents are discharged, the levels of most parameters were brought within permissible limits. From the study, it was apparent that Bacillus cereus and Pseudomonas aeruginosa have the ability to grow and survive in refinery effluents

Muon Collider

Both e+e- and {\mu}+{\mu}- colliders have been proposed as possible candidates for a lepton collider to complement and extend the reach of the Large Hadron Collider (LHC) at CERN. The physics program that could be pursued by a new lepton collider (e+e- or {\mu}+{\mu}-) with sufficient luminosity would include understanding the mechanism behind mass generation and electroweak symmetry breaking; searching for, and possibly discovering, supersymmetric particles; and hunting for signs of extra spacetime dimensions and quantum gravity. However, the appropriate energy reach for such a collider is currently unknown, and will only be determined following initial physics results at the LHC. It is entirely possible that such results will indicate that a lepton collider with a collision energy well in excess of 1 TeV will be required to illuminate the physics uncovered at LHC. Such a requirement would require consideration of muons as the lepton of choice for such a collider.Comment: v.2., 6 pp. To appear in the 2nd edition of the book Elementary Particles, Landolt-Boernstein Series published by Springer. arXiv admin note: text overlap with arXiv:physics/9901022 by other autho

Optical-phonon resonances with saddle-point excitons in twisted-bilayer graphene

Twisted-bilayer graphene (tBLG) exhibits van Hove singularities in the density of states that can be tuned by changing the twisting angle $\theta$. A $\theta$-defined tBLG has been produced and characterized with optical reflectivity and resonance Raman scattering. The $\theta$-engineered optical response is shown to be consistent with persistent saddle-point excitons. Separate resonances with Stokes and anti-Stokes Raman scattering components can be achieved due to the sharpness of the two-dimensional saddle-point excitons, similar to what has been previously observed for one-dimensional carbon nanotubes. The excitation power dependence for the Stokes and anti-Stokes emissions indicate that the two processes are correlated and that they share the same phonon.Comment: 5 pages, 6 figure

Lattice dynamics localization in low-angle twisted bilayer graphene

A low twist angle between the two stacked crystal networks in bilayer graphene enables self-organized lattice reconstruction with the formation of a periodic domain. This superlattice modulates the vibrational and electronic structures, imposing new rules for electron-phonon coupling and the eventual observation of strong correlation and superconductivity. Direct optical images of the crystal superlattice in reconstructed twisted bilayer graphene are reported here, generated by the inelastic scattering of light in a nano-Raman spectroscope. The observation of the crystallographic structure with visible light is made possible due to lattice dynamics localization, the images resembling spectral variations caused by the presence of strain solitons and topological points. The results are rationalized by a nearly-free-phonon model and electronic calculations that highlight the relevance of solitons and topological points, particularly pronounced for structures with small twist angles. We anticipate our discovery to play a role in understanding Jahn-Teller effects and electronic Cooper pairing, among many other important phonon-related effects, and it may be useful for characterizing devices in the most prominent platform for the field of twistronics.Comment: 9 pages, 8 figure

Status of Muon Collider Research and Development and Future Plans

The status of the research on muon colliders is discussed and plans are outlined for future theoretical and experimental studies. Besides continued work on the parameters of a 3-4 and 0.5 TeV center-of-mass (CoM) energy collider, many studies are now concentrating on a machine near 0.1 TeV (CoM) that could be a factory for the s-channel production of Higgs particles. We discuss the research on the various components in such muon colliders, starting from the proton accelerator needed to generate pions from a heavy-Z target and proceeding through the phase rotation and decay ($\pi \to \mu \nu_{\mu}$) channel, muon cooling, acceleration, storage in a collider ring and the collider detector. We also present theoretical and experimental R & D plans for the next several years that should lead to a better understanding of the design and feasibility issues for all of the components. This report is an update of the progress on the R & D since the Feasibility Study of Muon Colliders presented at the Snowmass'96 Workshop [R. B. Palmer, A. Sessler and A. Tollestrup, Proceedings of the 1996 DPF/DPB Summer Study on High-Energy Physics (Stanford Linear Accelerator Center, Menlo Park, CA, 1997)].Comment: 95 pages, 75 figures. Submitted to Physical Review Special Topics, Accelerators and Beam