Tropical Legumes to Augment Forage Yield in the Eastern Caribbean

Little information is available on the contribution of tropical legumes to forage yield in the eastern Caribbean. This study assessed the effects of three grazing cycles (GC; 5, 10 and 15-wk) on seasonal dry matter (DM) yield of binary mixtures of the tropical legumes glycine (Neonotonia wightii) and desmanthus (Desmanthus virgatus) with “Bambatsi” (Panicum coloratum) and “Mottgrass” (Pennisetum purpureum Shum.). Plant responses were assessed by mob-grazing with sheep (12-24 hrs; to average stubble height of 15-cm) for each GC. Grazing cycles affected yield of both the legume and grass component (P\u3c 0.05). At GC of 5-weeks, forage DM ranged from 1.5-2.0 Mg ha-1 in both years. Forage yield increases at10 and 15-wk for both grass and legume were two-fold. Legume yield contribution ranged from 12-32 percentage by weight in 1997 and 33 to 58% in 1999. Desmanthus dominated bambatsi swards. Glycine associated well with both bambatsi and Mottgrass. Because of its aggressive shrub-growth habit, Desmanthus is better suited for use as hedgerows in native pastures. Glycine is more compatible to use as binary mixtures with grasses to increase yield and nutritive value in the eastern Caribbean

Sewage Sludge as an Alternative Fertilizer for Tropical Pasture Grasses

This study was conducted to determine the effects of source (Liquid sludge at pH 7 (LS7) or pH 11 (LS11), cake biosolid (CB) or ammonium nitrate (AM)) by rate (0, 80 or 160 kg ha-1) of N application on bahiagrass forage yield, quality and tissue composition. Same plots were fertilized in March 1998 and 1999. Forage yield was measured at 30 d intervals from May through November and subsamples were dried and ground for quality analyses. The 2-year mean forage yield was similar for AM, LS7 and LS 11 fertilizer applications. Forage yield from CB application was 30% lower than yield from other N sources at comparable N rates. Forage crude protein content was highest for the AM treatment only during the initial harvest. Tissue concentrations of plant nutrients were increased by organic sources of fertilizer. These results, in conjunction with data from soil and groundwater analyses, suggest that processed domestic septage could be a safe and inexpensive substitute to inorganic fertilizer for tropical pasture grasses

Effect of water treated and urea treated neem (Azadirachta indica) kernel cake as protein supplement on haematological, biochemical and carcass characteristics of broiler chickens

In a study to evaluate the carcass haematological and biochemical characteristics of broiler birds fed graded levels of water and urea-treated neem kernel cake (NKC), 300 day-old broilers (Cobb, 500) were randomly assigned to five dietary treatments for 56 days. Water and feed were fed adlibitum. The diets were formulated to be isonitrogenous to replace soya bean meal at 0 per cent neem kernel cake (NKC), 5 per cent water treated neem kernel cake (WNKC), 10 per cent WNKC, 5 per cent water and urea treated neem kernel cake (WUNKC) and 10 per cent WUNKC for diets 1,2,3,4, and 5, respectively. The results showed that average daily feed intake (ADFI), average body weight gain (ABWG), average daily water intake (ADWI), feed conversion ratio (FCR), and feed cost kg-1 live weight gain were significantly (P = 0.05) different across dietary treatments. With the exception of the red blood cell (RBC), globulin and total protein, all the haematological parameters (white blood cell, haemoglobin, PCV, MCH, MCHC and MCV) and biochemical parameters (albumen, cholesterol, HDL, LDL and TGS) measured were not significantly (P = 0.05) different across the dietary treatments. Results on carcass parameters were significantly (P = 0.05) different among the dietary treatments. Liver, gizzard, heart, dressed weight, and intestinal weights of birds on the test diets were significantly (P = 0.05) different from those on the control diet

The perception and preference of consumers for local poultry meat in the Kumasi metropolis of Ghana

The study examined the preference for and perception of consumers concerning the locally produced poultry meat in the Kumasi metropolis in the Ashanti Region of Ghana. Fifty consumers were randomly selected. The results demonstrated that most consumers preferred the local poultry meat to the imported meat. The logit estimates indicated that factors such as age, gender, cold store, cut parts, and healthiness significantly influence consumers’ preference for the locally produced poultry meat. Age, gender and healthiness positively influence consumer’s preference, while cold store and cut parts negatively associated with consumer preference for local poultry meat. On the perception, 68 per cent of the consumers strongly agreed that local poultry meat is tough, while 58 per cent alleged that imported poultry meat is rather tough. In terms of affordability, 46 per cent of the consumers disagreed that local poultry meat is affordable, while 52 per cent strongly objected to the idea that imported poultry meat is relatively cheaper. In terms of availability, 54 per cent of the consumers admitted that local poultry meat is not readily available, while 58 per cent indicated that imported poultry meat is readily available and can be obtained at anytime. Sixty per cent of the respondents were of the opinion that local poultry meat taste better, while 46 per cent of the respondents indicated that imported poultry meat do not taste better. This was probably due to long period of refrigeration, which reduced its freshness and taste and made it less healthy for consumption. It is suggested that the government should subsidize the cost of inputs for the local poultry industry, since the results indicated that high cost was the major factor that influenced consumers’ purchasing decision

Adoption of improved cowpea (Vigna unguiculata (L.) Walp) technologies in Ghana

The Ghana Grains Development Project has developed and disseminated improved cowpea production technologies to farmers in Ghana since 1985. These technologies were improved varieties, row planting, and the use of pre- and post-flowering insecticides. To assess the extent of adoption of these technologies by farmers, eight cowpea production districts were surveyed across four agro-ecological zones in 1995 in Ghana. A random sample of 313 cowpea farmers participated in the survey. Results indicated that 70 per cent used recommended pre-flowering insecticides, and 26 per cent adopted post-flowering insecticides across the ecological zones. The highest adoption rate for improved varieties was observed in the Forest Zone (97 %). The adoption of row planting was highest in the Forest Zone (100 %), followed by the Guinea Savanna Zone (92 %), and lowest in the Coastal Savanna Zone (58 %). Most farmers agreed that by adopting improved production technologies, their yields and overall production increased, and they made more profit from cowpea cultivation.The whole family participates in various activities associated with cowpea production. However, men do strenuous work like land preparation, weeding and spraying of insecticides, while women and children participate in less strenuous work such as planting, harvesting and threshing. Le Project de Développement des Grains du Ghana a developpé et disséminé les technologies de la production de dolique amélioré aux cultivateurs au Ghana depuis 1985. Ces technologies étaient les variétés améliorées, la culture en lignes et usage d'insecticides avant et après la floraison. Pour évaluer le degré d'adoption de ces technologies par les cultivateurs, un sondage a été entrepris en 1995 en 8 districts de production de dolique à travers quatre zones agro-écologique au Ghana. Un échantillon pris au hasard de 313 cultivateurs de dolique participaient au sondage. Les résultats indiquaient que 70% utilisaient les insecticides d'avant floraison recommandés et 26% adoptaient les insecticides d'après floraison à travers les zones écologiques. La proportion d'adoption la plus élevée pour les variétés améliorées était observée dans la zone forestière (97 %). L'adoption de la culture en lignes est la plus élevée dans la zone forestière (100 %) et suivi par la zone savane-guinéenne (92 %) et la plus faible dans la zone savane-littorale (58 %). La plupart de cultivateurs étaient du même avis que par l'adoption des technologies de production améliorée, leur rendements et la production globale augmentaient, et ils avaient tiré plus de bénéfice de la production de dolique. Toute la famille participent aux activités différentes liées à la production de dolique. Cependant le travail ardu tel que la préparation de terre, le désherbage et la pulvérisation d'insecticide sont faites par les hommes alors que les femmes et les enfants participent aux travaux moins ardus tels que la plantation, la moisson et la battage. Ghana Journal of Agricultural Science Vol. 39 (1) 2006: pp. 25-3

A travel behaviour change framework for the city of Cape Town

Rapid increase in car ownership, and associated externalities, have motivated Transport for Cape Town (TCT) to develop strategies to curb growth in car use, particularly growth in Single Occupancy Vehicles (SOVs) in and out of the city centre. This paper reports upon the results of a project which sought to develop a framework to guide TCT?s Travel Demand Management (TDM) strategy, based upon a review of theory and a meta-analysis of empirical evidence. This framework includes a TDM strategy scenario in which a set of phased interventions seek to reduce the share of weekday SOV traffic into the city centre by 10% after five years. It is argued, from a theoretical perspective, that the keys to effective TDM intervention include: identifying which trip decision elements should be targeted in the short and long term; recognising which TDM measures are likely to have the greatest impact upon these decision elements; understanding which groups are most susceptible to change so that TDM measures might be targeted strategically; understanding the triggers which lead individuals to deliberately reappraise their travel decisions; influencing the variables that create the necessary circumstances that prompt decisions leading to the desired pattern of ?asymmetry?; and ?locking in? the vehicle kilometres travelled and other benefits, when they accrue. On the basis of the meta-analysis of secondary data, it is contended that financial charging can be expected to register the highest reduction in SOV mode use share, at around 15% at a precinct scale, followed by financial incentives, regulatory and voluntary measures at around 5%, 3% and 2% respectively. It is suggested that with the correct packaging, sequencing, targeting and resourcing, a 10% reduction in the SOV mode share of traffic travelling in and out of the city centre could be achieved.Paper presented at the 34th Annual Southern African Transport Conference 6-9 July 2015 "Working Together to Deliver - Sakha Sonke", CSIR International Convention Centre, Pretoria, South Africa.The Minister of Transport, South AfricaTransportation Research Board of the US

Adjuvant chemotherapy with or without bevacizumab in patients with resected non-small-cell lung cancer (E1505): an open-label, multicentre, randomised, phase 3 trial.

BackgroundAdjuvant chemotherapy for resected early-stage non-small-cell lung cancer (NSCLC) provides a modest survival benefit. Bevacizumab, a monoclonal antibody directed against VEGF, improves outcomes when added to platinum-based chemotherapy in advanced-stage non-squamous NSCLC. We aimed to evaluate the addition of bevacizumab to adjuvant chemotherapy in early-stage resected NSCLC.MethodsWe did an open-label, randomised, phase 3 trial of adult patients (aged ≥18 years) with an Eastern Cooperative Oncology Group performance status of 0 or 1 and who had completely resected stage IB (≥4 cm) to IIIA (defined by the American Joint Committee on Cancer 6th edition) NSCLC. We enrolled patients from across the US National Clinical Trials Network, including patients from the Eastern Cooperative Oncology Group-American College of Radiology Imaging Network (ECOG-ACRIN) affiliates in Europe and from the Canadian Cancer Trials Group, within 6-12 weeks of surgery. The chemotherapy regimen for each patient was selected before randomisation and administered intravenously; it consisted of four 21-day cycles of cisplatin (75 mg/m2 on day 1 in all regimens) in combination with investigator's choice of vinorelbine (30 mg/m2 on days 1 and 8), docetaxel (75 mg/m2 on day 1), gemcitabine (1200 mg/m2 on days 1 and 8), or pemetrexed (500 mg/m2 on day 1). Patients in the bevacizumab group received bevacizumab 15 mg/kg intravenously every 21 days starting with cycle 1 of chemotherapy and continuing for 1 year. We randomly allocated patients (1:1) to group A (chemotherapy alone) or group B (chemotherapy plus bevacizumab), centrally, using permuted blocks sizes and stratified by chemotherapy regimen, stage of disease, histology, and sex. No one was masked to treatment assignment, except the Data Safety and Monitoring Committee. The primary endpoint was overall survival, analysed by intention to treat. This trial is registered with ClinicalTrials.gov, number NCT00324805.FindingsBetween June 1, 2007, and Sept 20, 2013, 1501 patients were enrolled and randomly assigned to the two treatment groups: 749 to group A (chemotherapy alone) and 752 to group B (chemotherapy plus bevacizumab). 383 (26%) of 1458 patients (with complete staging information) had stage IB, 636 (44%) had stage II, and 439 (30%) had stage IIIA disease (stage of disease data were missing for 43 patients). Squamous cell histology was reported for 422 (28%) of 1501 patients. All four cisplatin-based chemotherapy regimens were used: 377 (25%) patients received vinorelbine, 343 (23%) received docetaxel, 283 (19%) received gemcitabine, and 497 (33%) received pemetrexed. At a median follow-up of 50·3 months (IQR 32·9-68·0), the estimated median overall survival in group A has not been reached, and in group B was 85·8 months (95% CI 74·9 to not reached); hazard ratio (group B vs group A) 0·99 (95% CI 0·82-1·19; p=0·90). Grade 3-5 toxicities of note (all attributions) that were reported more frequently in group B (the bevacizumab group) than in group A (chemotherapy alone) were overall worst grade (ie, all grade 3-5 toxicities; 496 [67%] of 738 in group A vs 610 [83%] of 735 in group B), hypertension (60 [8%] vs 219 [30%]), and neutropenia (241 [33%] vs 275 [37%]). The number of deaths on treatment did not differ between the groups (15 deaths in group A vs 19 in group B). Of these deaths, three in group A and ten in group B were considered at least possibly related to treatment.InterpretationAddition of bevacizumab to adjuvant chemotherapy did not improve overall survival for patients with surgically resected early-stage NSCLC. Bevacizumab does not have a role in this setting and should not be considered as an adjuvant therapy for patients with resected early-stage NSCLC.FundingNational Cancer Institute of the National Institutes of Health

Proteomic profile of KSR1-regulated signalling in response to genotoxic agents in breast cancer

Kinase suppressor of Ras 1 (KSR1) has been implicated in tumorigenesis in multiple cancers, including skin, pancreatic and lung carcinomas. However, our recent study revealed a role of KSR1 as a tumour suppressor in breast cancer, the expression of which is potentially correlated with chemotherapy response. Here, we aimed to further elucidate the KSR1-regulated signalling in response to genotoxic agents in breast cancer. Stable isotope labelling by amino acids in cell culture (SILAC) coupled to high-resolution mass spectrometry (MS) was implemented to globally characterise cellular protein levels induced by KSR1 in the presence of doxorubicin or etoposide. The acquired proteomic signature was compared and GO-STRING analysis was subsequently performed to illustrate the activated functional signalling networks. Furthermore, the clinical associations of KSR1 with identified targets and their relevance in chemotherapy response were examined in breast cancer patients. We reveal a comprehensive repertoire of thousands of proteins identified in each dataset and compare the unique proteomic profiles as well as functional connections modulated by KSR1 after doxorubicin (Doxo-KSR1) or etoposide (Etop-KSR1) stimulus. From the up-regulated top hits, several proteins, including STAT1, ISG15 and TAP1 are also found to be positively associated with KSR1 expression in patient samples. Moreover, high KSR1 expression, as well as high abundance of these proteins, is correlated with better survival in breast cancer patients who underwent chemotherapy. In aggregate, our data exemplify a broad functional network conferred by KSR1 with genotoxic agents and highlight its implication in predicting chemotherapy response in breast cancer

Effects of Polymer 3D Architecture, Size, and Chemistry on Biological Transport and Drug Delivery In Vitro and in Orthotopic Triple Negative Breast Cancer Models

© 2020 Wiley-VCH GmbH The size, shape, and underlying chemistries of drug delivery particles are key parameters which govern their ultimate performance in vivo. Responsive particles are desirable for triggered drug delivery, achievable through architecture change and biodegradation to control in vivo fate. Here, polymeric materials are synthesized with linear, hyperbranched, star, and micellar-like architectures based on 2-hydroxypropyl methacrylamide (HPMA), and the effects of 3D architecture and redox-responsive biodegradation on biological transport are investigated. Variations in “stealth” behavior between the materials are quantified in vitro and in vivo, whereby reduction-responsive hyperbranched polymers most successfully avoid accumulation within the liver, and none of the materials target the spleen or lungs. Functionalization of selected architectures with doxorubicin (DOX) demonstrates enhanced efficacy over the free drug in 2D and 3D in vitro models, and enhanced efficacy in vivo in a highly aggressive orthotopic breast cancer model when dosed over schedules accounting for the biodistribution of the carriers. These data show it is possible to direct materials of the same chemistries into different cellular and physiological regions via modulation of their 3D architectures, and thus the work overall provides valuable new insight into how nanoparticle architecture and programmed degradation can be tailored to elicit specific biological responses for drug delivery

Bibliometric analysis of authorship trends and collaboration dynamics over the past three decades of BONE's publication history

The existence of a gender gap in academia has been a hotly debated topic over the past several decades. It has been argued that due to the gender gap, it is more difficult for women to obtain higher positions. Manuscripts serve as an important measurement of one's accomplishments within a particular field of academia. Here, we analyzed, over the past 3 decades, authorship and other trends in manuscripts published in BONE, one of the premier journals in the field of bone and mineral metabolism. For this study, one complete year of manuscripts was evaluated (e.g. 1985, 1995, 2005, 2015) for each decade. A bibliometric analysis was then performed of authorship trends for those manuscripts. Analyzed fields included: average number of authors per manuscript, numerical position of the corresponding author, number of institutions collaborating on each manuscript, number of countries involved with each manuscript, number of references, and number of citations per manuscript. Each of these fields increased significantly over the 30-year time frame (p < 10− 6). The gender of both the first and corresponding authors was identified and analyzed over time and by region. There was a significant increase in the percentage of female first authors from 23.4% in 1985 to 47.8% in 2015 (p = 0.001). The percentage of female corresponding authors also increased from 21.2% in 1985 to 35.4% in 2015 although it was not significant (p = 0.07). With such a substantial emphasis being placed on publishing in academic medicine, it is crucial to comprehend the changes in publishing characteristics over time and geographical region. These findings highlight authorship trends in BONE over time as well as by region. Importantly, these findings also highlight where challenges still exist