Burn wound healing effects of the root hydroethanolic extract of Cochlospermum planchonii in mice

Cochlospermum planchonii is a plant traditionally used in Togo to treat burn wounds. The present study aimed at evaluating the effect of the root hydroethanolic extract of Cochlospermum planchonii on the skin burn wounds. Burns were induced in ICR mice by applying on the skin of the dorsal region with a metal stick (0.7 cm diameter) preheated at 100 °C for 10 seconds. Four (4) groups of 8 mice were formed, Control Group (I); Group II, III and IV treated respectively with C. planchonii gel 2.5%, 5%, and Brulex® (Zinc oxide 15% cream). The surfaces of the wounds were evaluated daily by the ImageJ software. Biopsies were performed on day six and twelve for hydroxyproline assays and histological examinations. On the 12th day, wound contraction rates were 44.12%, 67.82%, 66.68% and 69.32% respectively for Group I, Group II, Group III and Group IV. Hydroxyproline levels on day 12 were significantly higher in samples of animals treated with C. planchonii 2.5% and 5% (+114,93 ± 1,61 and +120,54 ± 7,22 of augmentation respectively, P<0.01 when compared to the control group). Histological examination confirmed these observations. C. planchonii root hydroethanolic extract could be a potentially natural remedy for burn wound

Antioxidant and nephroprotection activities of Combretum micranthum: A phytochemical, in-vitro and ex-vivo studies

Management of chronic renal failure is exceedingly expensive. Despite of encouraging experimental outcomes, there is a lack of potent nephroprotective drugable molecules in a clinics or market. To develop a nephroprotective phytomedicine, the present study was designed to do a literature survey on reported phytochemical and biological analysis of Combretum micranthum and to carry out chemoprofiling, in-vitro antioxidant and ex-vivo nephroprotective capacity of the title plant. The phytochemical and biological activity survey of C. micranthum has reveals the presence of many bioactive compounds such as flavonoids, terpenoids, steroids and alkaloids with many biological activities. Phytochemical investigation re-confirmed the presence of these compounds. Hydroalcoholic extract of C. micranthum (CM extract) showed a strong antioxidant activity by scavenging AAPH, DPPH, nitric oxide, hydrogen peroxide and chelating metal ions. CM extract exhibited significant (P < 0.001) dose dependent inhibition of ferric chloride-ascorbic acid induced lipid peroxidation. Diabetic nephropathy is a serious and common complication leading to end stage renal disease. Therefore, in the present study, glucose-induced toxicity was also studied in human embryonic kidney cells (HEK-293) as an in vitro model for diabetic nephropathy. The results showed that exposure of cells to high glucose (100 mM) for 72 h significantly reduced the cell viability resulting in morphological changes such as cell shrinkage, rounded cell shape and cytoplasmic vacuolation. Treatment with CM extract at 10 and 25 μg/mL resulted in significant improvement in cell viability from 10 to 23% compared to the high glucose control. This study demonstrated the potential antioxidant and nephroprotective properties of C. micranthum, justifying its traditional use in the treatment of various diseases

Antioxidant, Anti-Inflammatory and Wound Healing Activities of Cochlospermum planchonii Hook. F.

Objective: The medicinal plant Cochlospermum planchonii Hook.f. is used in the management of various ailments in Togolese pharmacopoeia. In this study, we aimed to evaluate the antioxidant and anti-inflammatory activities of roots and leaves of C. planchonii, and burn wound healing activity of its leaf hydroethanolic extracts in rodents. Materials and Methods: Antioxidant activities were assessed using Phosphomolybdenum assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) test and the reducing power assay. Visceral pain model, formaldehyde-induced paw edema and vascular permeability test were performed to evaluate anti-inflammatory activities in vivo. Burns were induced in rats by applying on the skin of the dorsal region an aluminum plaque preheated to 100°C for 10 seconds. Animals were treated topically with empty Carbopol gel, C. planchonii leaves extract 2.5 and 5 % in Carbopol gel, and Brulex® (Zinc oxide 15 % cream). Results: C. planchonii extracts exhibited good antioxidant capacities close to standard compound, ascorbic acid. Leaves and root hydroethanolic extracts (1000 mg/kg), compared to control animals, significantly reduced the number of writhings (P&lt;0.001) and the volume of paw edema (P&lt;0.001). Similarly, both roots and leaf extracts at 1000 mg/kg have significantly inhibited vascular permeability by approximately 50% compared to the control group. C. planchonii leaves hydroethanolic extract 2.5 and 5 % in Carbopol enhanced wound healing via significantly increased contraction rates (78.63 ± 1.57 and 79.68 ± 1.48 respectively on day 12, P&lt;0.001), confirmed by histological observations. Conclusion: C. planchonii can promote burn healing due to anti-inflammatory, antioxidant and antimicrobial properties of the plant. Keywords: Cochlospermum planchonii, inflammatory, antioxidant, edema, burn woun