Disruptive and Nondisruptive Selection for Bulk Oat Populations

A mixture of F3 seeds from 75 oat crosses was divided into four lots, with one being propagated in central Iowa for nine generations (i.e., stationary line of descent) and three being propagated in a rotational pattern in central, southern, and northern Iowa in successive generations (i.e., disruptively selected line of descent). An evaluation experiment was conducted to test whether any changes in genotypic frequencies were caused by the two propagation procedures. Increases in the means of yield traits occurred, but the magnitude and timing of the changes varied among lines of descent. The changes in the stationary and rotational lines of descent were indistinguishable. There was some trend for reduced genotypic variances for most traits with advancing generations. Probably the disruptive selection scheme did not cause differential results from the stationary one because the selection pressure due to differences in propagation sites was mild relative to the pressure due to differences in weather patterns during the years of the propagation period

HUMAN RESOURCE MANAGEMENT PRACTICES: CATALYSTS FOR REAL ENTREPRENEURIAL FIRMS’ GROWTH IN SOUTHWEST, NIGERIA

The study examined the role of Human Resource Management Practices (HRMPs) on entrepreneurial firms’ growth in South West Nigeria.  This was to entrenching a set of adequate human resource management practices that can enhance the growth objective of entrepreneurial firms. A structured questionnaire was used to elicit information on relevant variables of the study. Data collected were analysed using descriptive and inferential statistics with the aid of IBM SPSS statistics version 21.  The results revealed that employees’ training (    = 31.03, s=8.6405); controlling (   = 25.95, s= 6.721)); organizing manpower (    = 23.20, s = 5.760); job analysis and design (   = 23.10, s = 5.883) were reasonably practiced among the firms under the study. Whereas motivating strategies (    = 21.5, s = 5.2905; recruitment and selection (   = 20.45, s = 4.831); and manpower planning (    = 18.72, s = 4.786) fell below the agreement criterion (    = 23.0).  However, it was established that there was a statistically significant influence of the predictor variables on the outcome variable (R2 = 0.837, adjusted R2 = 0.825).  Therefore, the study concluded that Human Resources Management Practices as an omnibus concept have significant influence on Entrepreneurial firms ‘growth. The study suggested that Human Resource Management Practices should be considered as a process by entrepreneurs as it galvanizes entrepreneurial firms' growth objectives.     &nbsp

Determinants of mortality in Nigerian children with severe anaemia

Background. Severe anaemia (haemoglobin concentration <50 g/l) is amajor cause of paediatric hospital admissions and deaths in the tropics.Objectives.To examine the pattern and predictors of mortalityamong severely anaemic children.Methods. A prospective cross-sectional study was conducted among children with severe anaemia at the Children’s Emergency Room of the University Teaching Hospital, Ado-Ekiti, Nigeria. Sociodemographiccharacteristics, clinical features, laboratory findings and co-morbidities of the survivors and those who died were compared by logistic regression analysis.Results. Of the 1 735 children admitted, 311 (17.9%) had severeanaemia, with a case-fatality rate of 9.3%. The presence of respiratorydistress (95% confidence interval (CI) 2.1 - 3.6, p=0.031); acidosis(95% CI 1.8 - 2.7, p=0.010); coma (95% CI 0.1 - 0.3, p=0.001);hypotension (95% CI 2.0 - 4.2, p=0.020); and bacteraemia (95% CI 3.1- 3.9; p=0.008) were the significant independent predictors of deathwith regression analysis.Conclusion. Early recognition with prompt and appropriate anticipatory intervention is essential to reduce mortality from severe anaemia

Impact of Attendance on Students’ Academic Performance in ICT Related Courses: Faculty of Engineering, Osun State Polytechnic, Iree.

The impact of lecture attendance on student’s academic performance was investigated based on student’s total score and frequency of class attendance of 598 randomly selected students. Attendance were marked by the lecturer at the end of each contact with the students, Data from the selected students were analyzed using regression and correlation statistical tools. The result of the analysis revealed a positive correlation between class attendance and students performance in the examination. It was finally recommended that attendance register should be marked by lecturers during contact periods. Moreover, a minimum requirement of 70% attendance should be enforced as a prerequisite for writing any end of semester examination. Keywords: Class attendance, student’s performance, student’s scor

Blue-emitting SiO₂-coated Si-doped ZnSeS quantum dots conjugated aptamer-molecular beacon as an electrochemical and metal-enhanced fluorescence biosensor for SARS-CoV-2 spike protein

The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which was first reported in early January 2020, continues to devastate the worlds public health system. Herein, we report on the development of a novel metal-enhanced fluorescence (MEF) and electrochemical biosensor for SARS-CoV-2 spike (S) protein. To develop the MEF biosensor, SiO2-coated Si-doped ZnSeS quantum dots (QDs) were newly synthesized and conjugated to an aptamer-molecular beacon (Apta-MB) probe. Thereafter, cationic AuNPs, used as a localised surface plasmon resonance (LSPR) signal amplifier, were self-assembled on the QDs-Apta-MB conjugate to form a QDs-Apta-MB-AuNP probe. To develop the electrochemical biosensor, the QDs-Apta-MB assay was carried out on a carbon nanofiber-modified screen-printed carbon electrode. Cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) were used to characterize the electrode surface whilst spectrophotometric, spectroscopic, fluorescence polarization and electron microscopic techniques were used to characterize the materials. Under optimal experimental conditions, the QDs binding to the Apta-MB, quenched the QDs’ fluorescence and with SARS-CoV-2 S protein binding to the Apta-MB, LSPR signal from cationic AuNPs of different sizes and shapes were used to tune the fluorescence signal to obtain enhanced sensitivity. On the other hand, using [Fe(CN)6]/K3−/4- buffered with NaAc-KAc-TrizmaAc-KSCN-Borax as the electrolyte solution, anodic peaks of the QDs from the CV and DPV plots were unravelled. Electrochemical detection of SARS-CoV-2 S protein was accomplished by a systematic increase in the QDs anodic peak current generated from the DPV plots. The limits of detection obtained for the SARS-CoV-2 S protein were 8.9 fg/mL for the QDs-Apta-MB-AuNP MEF probe and ∼0.5 pg/mL for the QDs-Apta-MB electrochemical probe. Detection of SARS-CoV-2 S protein in saliva was demonstrated using the QDs-Apta-MB-AuNP MEF probe

EVALUATION OF KNOWLEDGE, ATTITUDE AND PRACTICES RELATING TO EBOLA VIRUS DISEASE (EVD) PREVENTION AND CONTROL IN OGUN STATE, NIGERIA

A cross sectional study was carried out from 1st June to 30th June, 2015, to assess the knowledge, attitude and practices related to the prevention and control of Ebola Virus Disease in Ogun State, Nigeria. A semi-structured questionnaire was used to gather data from 300 respondents selected by multistage sampling technique across four local governments in the state. The data collected included socio-demographics, awareness, knowledge, practices and misconceptions about EVD among the respondents. Knowledge of respondents about EVD was assessed on 20 items with a 3-points rating scale of good >14, fair 10-14 and poor <10. Of the 308 respondents that took part in the study, 111 (36%) were males and 127 (41.2%) rural dwellers and 43 (14%) of the respondents were between the ages of 9-18, 139 (45.1%) were between the ages of 19-25, 83 (26.9%) were between the ages of 26-35 and the remaining 43 (14%) were above 35 years of age. Five (1.6%) of the respondents had no formal education, 9 (2.9%) had primary education, 133 (43.2%) had secondary education and 161 (52.3%) had tertiary education. All the respondents (100%) have heard of Ebola.  Only 2.6% had poor knowledge of Ebola with 12% being able to identify four misconceptions surrounding Ebola transmission and prevention. Respondents in the study reported various changes in practices such as avoidance of physical contact (33.4%), regular hand washing with soap and water (69.2%) and hand cleaning with other disinfectants (61.7%), nevertheless 73.1% of the respondents will not report infected member of the family. Television was the major source of receiving information with the internet being the trusted source of receiving information on Ebola by the respondents. In Ogun State, awareness and comprehensive knowledge on Ebola Virus Disease transmission is high with good knowledge on misconceptions. There is need to emphasize importance of reporting suspected case(s) to the hospital 

Insight into the electrochemical performance of heavy metal-free quantum dots in different buffered ferricyanide/ferrocyanide redox systems

The ferricyanide/ferrocyanide K3[Fe(CN)6]/K4[Fe(CN)6] redox couple is a widely utilized redox probe in electrochemistry. Buffering the K3[Fe(CN)6]/K4[Fe(CN)6] system has become one of the ways to generate improved electron transfer rates and stable electrochemical systems. In recent years, semiconductor quantum dots (QDs) have gained popularity as nanotags in electrochemical applications. Herein, we report on the comparative electrochemical behaviour of cadmium-free AuZnFeSeS quantum dots (QDs)-modified screen-printed carbon electrode (SPCE) in different buffered K3[Fe(CN)6]/K4[Fe(CN)6] electrolyte solutions using voltammetry and electrochemical impedance spectroscopy (EIS) techniques. Our results showed that the QDs/SPCE exhibited faster electron transfer rates, lower energy barrier and lower charge transfer resistance (Rct) in the studied buffered electrolyte solution

Differential effects of PDCD4 depletion on protein synthesis in myoblast and myotubes

BACKGROUND: Reduced muscle mass is a hallmark of metabolic diseases like diabetes and cancer. The mammalian (mechanistic) target of rapamycin complex 1/S6 kinase 1 (mTORC1/S6K1) pathway is critical to the regulation of muscle protein synthesis and mass but its mechanism of action is not completely understood. RESULTS: Using L6 myotubes, we characterized the regulation of programmed cell death 4 (PDCD4), a recently described substrate of S6K1. The abundance, but not Ser67 phosphorylation, of PDCD4 was sensitive to amino acid and serum deprivation: values in starved cells were 4.5X of control (P < 0.001). Refeeding had opposite effects. Growth factors, compared to amino acids, appeared more critical in regulating PDCD4 abundance. Furthermore, inhibition of mTORC1 or the proteasome prevented the refeeding-associated decrease in PDCD4 abundance. Amino acid and serum deprivation significantly increased PDCD4 binding to eIF4A (P < 0.05); this was reversed during refeeding. PDCD4 depletion by RNA interference had no significant effect on phenylalanine incorporation into myotube mixed proteins in control cells but further suppressed (30%) this measure in nutrient-deprived cells (P < 0.0005). This was not observed in myoblasts. In starved myotubes, PDCD4 depletion further reduced the association of eIF4G with eIF4E. CONCLUSION: Our data suggest that in myotubes, PDCD4 abundance is sensitive to nutritional manipulation in an mTORC1 and proteasome depended manner. Furthermore, the role of PDCD4 in regulating protein synthesis appears dependent on the developmental state of the cell