"Provincial Dialects of the North of England" Appendix VI. "Further Remarks upon the Dialects of the Northern Dales." Appendix VII.

Glosario. -- Varia. -- Yorkshire. -- Inglés del Norte de Inglaterra. -- Pertenece a la Colección Varia 1800-1950 de The Salamanca Corpus. -- Adam Sedgwick, 1785-1873. -- "Provincial Dialects of the North of England" Appendix VI. "Further Remarks upon the Dialects of the Northern Dales." Appendix VII. in A Memorial by the Trustees of Cowgill. -- 1868.[ES]Descripción de la fonología de los dialectos del Norte de Inglaterra y de Dent, en Yorkshire, y breve glosario. [EN]Description of the phonology of the Northern dialects and of the Dent dialect, in Yorkshire, and short glossary

Homophobia and heterosexism

“Homophobia” is a widely understood term referring to antihomosexual attitudes and practices, but terms such as “homophobia,” “heterosexism,” and “heteronormativity” point to different ideas of what “homosexual” means, and where opposition to same-sex relations originates. Gayle Rubin, relying on structural anthropology, proposes that it arises as a disciplinary mechanism used by men to exercise control over women’s reproductive power in families. Gender panic theory focuses particularly on how defensiveness against losing male status and privilege generates homophobia. Sociohistorical theories examine how homophobia increases or decreases according to the symbolic placement of lesbian, gay, bisexual, and transgender people in the social status system. In the early twenty-first century, contradictory trends have led to improved citizenship rights for LGBT people in some countries, while others have reinforced or increased state and social violence against their LGBT populations

Enhancement of sonochemical production of hydroxyl radicals from pulsed cylindrically converging ultrasound waves

Sonochemistry is the use of ultrasound to generate highly reactive radical species through the inertial collapse of a gas/vapour cavity and is a green alternative for hydrogen production, wastewater treatment, and chemical synthesis and modifications. Yet, current sonochemical reactors often are limited by their design, resulting in low efficacy and yields with slow reaction kinetics. Here, we constructed a novel sonochemical reactor design that creates cylindrically converging ultrasound waves to create an intense localised region of high acoustic pressure amplitudes (15 MPaPKPK) capable of spontaneously nucleating cavitation. Using a novel dosimetry technique, we determined the effect of acoustic parameters on the yield of hydroxyl radicals (HO·), HO· production rate, and ultimately the sonochemical efficiency (SE) of our reactor. Our reactor design had a significantly higher HO· production rate and SE compared to other conventional reactors and across literature

Enhanced Colorimetric Differentiation between Staphylococcus aureus and Pseudomonas aeruginosa Using a Shape-Encoded Sensor Hydrogel

Herein, we demonstrate a combined fluorescent probe/shape-encoded hydrogel strategy for the fast, sensitive, and selective detection of bacterial species via their characteristic enzymes. A poly(vinyl alcohol) (PVA) hydrogel loaded with the fluorescent probe N,N′-(3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-3′,6′-diyl)bis(2,2,3,3,3-pentafluoropropanamide) (ACS-HNE) was designed for the detection of elastase, an enzyme produced by Pseudomonas aeruginosa. Likewise, a chitosan-derived hydrogel was loaded with the fluorescent probe 4-methylumbelliferyl-α-d-glucopyranoside (MUD) by entrapment for the selective detection of α-glucosidase, an enzyme produced by Staphylococcus aureus. For an observation time of 60 min, limits of detection (LODs) of ≤20 nM for elastase and ≤30 pM for α-glucosidase were obtained, which in the latter case is 3 orders of magnitude better than related chitosan systems with covalently coupled substrate. To illustrate the potential utility of these highly sensitive sensor hydrogels as a simple point-of-care test system, shaped hydrogel slabs representing the letters P and S were manufactured to detect P. aeruginosa and S. aureus, respectively. These shapes were shown to provide an additional unique color code under UV illumination corresponding to the characteristic enzyme produced by the corresponding bacteria. This study shows potential for the future development of an effective and simple point-of-care test for the rapid identification of bacterial species that can be operated by nonspecialists

Selective electrochemiluminescent sensing of saccharides using boronic acid-modified coreactant

We report a strategy for modulating the electrogenerated chemiluminescence (ECL) response by integrating a boronic acid to the chemical structure of coreactants. Excellent selectivity for d-glucose was achieved by tuning the linker length of a bis-boronic acid amine coreactant.</p

Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols

Two ‘turn on’ TCF-based fluorescence probes were developed for the detection of biological thiols (TCF-GSH and TCFCl-GSH). TCF-GSH was shown to have a high sensitivity towards glutathione (GSH) with a 0.28 μM limit of detection. Unfortunately, at higher GSH concentrations the fluorescence intensity of TCF-GSH decreased and toxicity was observed for TCF-GSH in live cells. However, TCFCl-GSH was shown to be able to detect GSH at biologically relevant concentrations with a 0.45 μM limit of detection. No toxicity was found for TCFCl-GSH and a clear ‘turn on’ with good photostability was observed for the exogenous addition of GSH, Cys and HCys. Furthermore, TCFCl-GSH was used to evaluate the effects of drug treatment on the levels of GSH in live cells

Limiting Pseudomonas aeruginosa Biofilm Formation Using Cold Atmospheric Pressure Plasma

We investigate the ability to disrupt and limit growth biofilms of Pseudomonas aeruginosa using application of cold atmospheric pressure (CAP) plasma. The effect of the bio-film's exposure to a helium (CAP) jet was assessed at varying time points during biofilm maturation. Results showed that the amount of time during biofilm growth that CAP pressure was applied has a crucial role on the ability of biofilms to mature and recover after CAP exposure. Intervention during the early stages of biofilm formation (0-8 h) results in a 4-5-log reduction in viable bacterial cells (measured at 24 h of incubation) relative to untreated biofilms. However, CAP treatment of biofilm at 12 h and above only results in a 2-log reduction in viable cells. This has potentially important implications for future clinical application of CAP to treat infected wounds