Evaluation of canonical siRNA and Dicer substrate RNA for inhibition of hepatitis C virus genome replication - a comparative study

Hepatitis C virus (HCV) frequently establishes persistent infections in the liver, leading to the development of chronic hepatitis and, potentially, to liver cirrhosis and hepatocellular carcinoma at later stages. The objective of this study was to test the ability of five Dicer substrate siRNAs (DsiRNA) to inhibit HCV replication and to compare these molecules to canonical 21 nt siRNA. DsiRNA molecules were designed to target five distinct regions of the HCV genome - the 5′ UTR and the coding regions for NS3, NS4B, NS5A or NS5B. These molecules were transfected into Huh7.5 cells that stably harboured an HCV subgenomic replicon expressing a firefly luciferase/neoR reporter (SGR-Feo-JFH-1) and were also tested on HCVcc-infected cells. All of the DsiRNAs inhibited HCV replication in both the subgenomic system and HCVcc-infected cells. When DsiRNAs were transfected prior to infection with HCVcc, the inhibition levels reached 99.5%. When directly compared, canonical siRNA and DsiRNA exhibited similar potency of virus inhibition. Furthermore, both types of molecules exhibited similar dynamics of inhibition and frequencies of resistant mutants after 21 days of treatment. Thus, DsiRNA molecules are as potent as 21 nt siRNAs for the inhibition of HCV replication and may provide future approaches for HCV therapy if the emergence of resistant mutants can be addressed

Caderno de imagens: a indumentária em adaptações de obras de Machado de Assis = Photo album: The clothing in adaptations of Machado de Assis's works

This scrapbook of images examines the author's work through the eyes of directors and production teams, collecting citations taken from his texts and adding comments about the clothing worn by characters. This convergence makes sense, since the author fictionalized, criticized and discussed the social attire and fashion of his time, as well as the costumes and sets of plays. He also dedicates various passages of his texts to descriptions of clothing and the physical features of character, mixing the poetry of the subject and the colors of human psychology to create a unique literary construction

LAYSI: A layered approach for SLA-violation propagation in self-manageable cloud infrastructures

Cloud computing represents a promising comput ing paradigm where computing resources have to be allocated to software for their execution. Self-manageable Cloud in frastructures are required to achieve that level of flexibility on one hand, and to comply to users' requirements speci fied by means of Service Level Agreements (SLAs) on the other. Such infrastructures should automatically respond to changing component, workload, and environmental conditions minimizing user interactions with the system and preventing violations of agreed SLAs. However, identification of sources responsible for the possible SLA violation and the decision about the reactive actions necessary to prevent SLA violation is far from trivial. First, in this paper we present a novel approach for mapping low-level resource metrics to SLA parameters necessary for the identification of failure sources. Second, we devise a layered Cloud architecture for the bottom-up propagation of failures to the layer, which can react to sensed SLA violation threats. Moreover, we present a communication model for the propagation of SLA violation threats to the appropriate layer of the Cloud infrastructure, which includes negotiators, brokers, and automatic service deployer. © 2010 IEEE

Interview with Beth Filipecki, costume designer for Capitu (2008) = Entrevista com Beth Filipecki, figurinista de Capitu (2008)

The costume designer and university professor tells how she has been bringing her two occupations together for over thirty years by permanently interacting with colleagues and students from both fields in an exchange of knowledge involving professors, directors, producers, researchers, actors, actresses, visual artists, set designers, lighting technicians, make-up technicians, assistants, seamstresses, tailors, prop masters and craftsmen. In the interview granted to Ana Cláudia Suriani da Silva and Mariana Millecco in Rio de Janeiro on September 22,2016, Filipecki discusses the stages involved in creating the costumes for literary characters and explains the thought and production process for the miniseries Capitu, directed by Luiz Fernando Carvalho, for which she created the costumes of some of the most famous and complex of Machado de Assis's creations. /

Mechanism of Reovirus Double-Stranded Ribonucleic Acid Synthesis In Vivo and In Vitro

The complementary strands of reovirus double-stranded ribonucleic acid (ds RNA) are synthesized sequentially in vivo and in vitro. In both cases, preformed plus strands serve as templates for the synthesis of the complementary minus strands. The in vitro synthesis of dsRNA is catalyzed by a large particulate fraction from reovirus-infected cells. Treatment of this fraction with chymotrypsin or with detergents which solubilize cellular membranes does not alter its capacity to synthesize dsRNA. The enzyme or enzymes responsible for dsRNA synthesis remain sedimentable at 10,000 × g after these enzyme or detergent treatments, indicating their particulate nature. Pretreatment of this fraction with ribonuclease, however, abolishes its ability to catalyze dsRNA synthesis, emphasizing the single-stranded nature of the template and its location in a structure permeable to ribonuclease. In contrast, the newly formed dsRNA is resistant to ribonuclease digestion at low salt concentrations and hence is thought to reside within a ribonuclease-impermeable structure

Water Framework Directive Intercalibration Technical Report: Lake phytobenthos ecological assessment methods

The European Water Framework Directive (WFD) requires the national classifications of good ecological status to be harmonised through an intercalibration exercise. In this exercise, significant differences in status classification among Member States are harmonized by comparing and, if necessary, adjusting the good status boundaries of the national assessment methods. Intercalibration is performed for rivers, lakes, coastal and transitional waters, focusing on selected types of water bodies (intercalibration types), anthropogenic pressures and Biological Quality Elements. Intercalibration exercises were carried out in Geographical Intercalibration Groups - larger geographical units including Member States with similar water body types - and followed the procedure described in the WFD Common Implementation Strategy Guidance document on the intercalibration process (European Commission, 2011). In a first phase, the intercalibration exercise started in 2003 and extended until 2008. The results from this exercise were agreed on by Member States and then published in a Commission Decision, consequently becoming legally binding (EC, 2008). A second intercalibration phase extended from 2009 to 2012, and the results from this exercise were agreed on by Member States and laid down in a new Commission Decision (EC, 2013) repealing the previous decision. Member States should apply the results of the intercalibration exercise to their national classification systems in order to set the boundaries between high and good status and between good and moderate status for all their national types. Annex 1 to this Decision sets out the results of the intercalibration exercise for which intercalibration is successfully achieved, within the limits of what is technically feasible at this point in time. The Technical report on the Water Framework Directive intercalibration describes in detail how the intercalibration exercise has been carried out for the water categories and biological quality elements included in that Annex. The Technical report is organized in volumes according to the water category (rivers, lakes, coastal and transitional waters), Biological Quality Element and Geographical Intercalibration group. This volume addresses the intercalibration of the cross-GIG phytobenthos ecological assessment methods

Introduction: innovation and small business

This paper introduces the special issue of Small Business Economics on Innovation. What binds the papers together is either their focus on the effect of firm size on the causes and consequences of innovation or their focus on the role small firms play in reshaping the industrial landscape

Regional innovation and spillover effects of foreign direct investment in China: a threshold approach

Using a data set on twenty-nine Chinese provinces for the period 1985–2008, this paper establishes a threshold model to analyse the relationship between spillover effects of foreign direct investment (FDI) and regional innovation in China. There is clear evidence of double-threshold effects of regional innovation on productivity spillovers from FDI. Specifically, only when the level of regional innovation reaches the minimum innovation threshold will FDI in the region begin to produce positive productivity spillovers. Furthermore, positive productivity spillovers from FDI will be substantial only when the level of regional innovation attains a higher threshold. The double threshold divides Chinese provinces into three super-regions in terms of innovation, with most provinces positioned within the middle-level innovation super-region. Policy implications are discussed