Insilico and Invitro anthelmintic properties of phytocompounds in Rostellularia quinquangularis (J. Koenig ex Roxb.) Nees

The present study aimed to evaluate the anthelmintic activity of various extracts of Rostellularia quinquangularis (R. quinquangularis) against adult Indian earthworms (Pheretima posthuma). Petroleum ether extract (PERQ), ethyl acetate extract (RQEA), and ethanol extract (RQEE) of R. quinquangularis were tested at different concentrations (10, 20, 50, and 100 mg/mL), along with the positive control (albendazole) and negative control (normal saline). Anthelmintic activity was assessed based on the duration of paralysis and mortality. The RQEE extract showed significant anthelmintic activity, with the highest activity observed at a concentration of 100 mg/mL, exhibiting paralysis time of 1.62 min and death times of 19.9 min, compared to the standard albendazole. Further, HR LC-MS analysis of the RQEE extract revealed the presence of various phytoconstituents based on m/z signals. Molecular docking analysis using AutoDock Vina indicated that Columbianetin, Dunnione, Cryptochlorogenic acid, Gaylussacin, Luvangetin, and Albendazole showed docking scores of -8.1, -7.9, -7.4, -7.3, -7.2, and -6.8 Kcal/mol, respectively. These results suggest that R. quinquangularis possesses potent anthelmintic activity, supporting its traditional use in medicinal practices.

High-throughput liquid chromatography tandem mass spectrometry method for simultaneous determination of fampridine, paroxetine, and quinidine in rat plasma: Application to in vivo perfusion study

A selective and high-throughput liquid chromatography–mass spectrometry method has been developed and validated for the simultaneous quantification of paroxetine, fampridine, and quinidine in rat plasma using imipramine as an internal standard. Following protein precipitation extraction, the analytes and internal standard were run on XBridge C18 column (150 mm × 4.6 mm, 5 μm) using a gradient mobile phase consisting of 5mM ammonium formate in water (pH 9.0) and acetonitrile in a flow gradience program. The precursor and product ions of the drugs were monitored on a triple quadrupole instrument operated in the positive ionization mode. The method was validated over a concentration range of 0.1–100 ng/mL for all the three analytes, with relative recoveries ranging from 69% to 82%. The intra- and interbatch precision (percent coefficient of variation) across four validation runs were less than 13.4%. The accuracy determined at four quality control (QC) levels (lower limit of quantitation, low QC, medium QC, and high QC) was within ±6.5% of coefficient of variation values. The method proved highly reproducible and sensitive, and was successfully applied in a pharmacokinetic study after single-dose oral administration to rats and also in perfusion study sample analysis