Does pendimethalin develop in pancreatic cancer induced inflammation?

Pendimethalin, one of the dinitroaniline group herbicides, is applied for controlling weeds in cereals,legumes and vegetable crops, and has been classified as possible human carcinogen. It is indicated thatpendimethalin should arise risks of developing some cancer types; however, there is no data on theeffects of pendimethalin on pancreatic cancer-induced inflammation. Injuries resulting from by acutepancreatitis attacks and inflammation are significant factors in the development of pancreatic cancer.Therefore, we investigated whether pendimethalin triggers inflammation as a mechanism of pancreaticcancer development. Parameters related to pancreatic activation, oxidative stress, and inflammationwere measured in the human pancreatic (PANC-1) cell line. In the range of 0e100mM, the levels ofchymotrypsin decreased. It should be indicated that the reason for the decrease in chymotrypsin may bethe high rates of cell death (20%) observed in the high concentration levels. We observed that pendi-methalin significantly induced oxidative damage, while levels of interleukin-6 (IL-6) and interleukin-8(IL-8) did not change. The obtained results may draw attention to the usage and possible toxic effectof pendimethalin due to oxidative damage induction; however, detailed inflammation mechanisms andother cancer pathways should be investigated

Effects of bentazone on lipid peroxidation and antioxidant systems in human erythrocytes in vitro

Bentazone, a benzothiadiazole herbicide, is widely used for a variety of crops including cereals, maize, peas, rice and soy beans. The concern for human health is stil very high because bentazone is continuously monitored in environment and several studies to evaluate its potential carcinogenic effects when chronic and high doses were administered to animals. We aimed to investigate the possible effects of bentazone on lipid peroxidation, levels of glutathione and activities of antioxidant enzymes in human erythrocytes in vitro. For that, erythrocyte were incubated with bentazone in different concentrations (0-50 nM) at 37 degrees C for 1 hr. Bentazone showed significant increase in the levels of malondialdehyde (MDA) at the highest concentration in erythrocytes as an index of lipid peroxidation. Besides, alterations in the levels of reduced glutathione (GSH) and activities of glutathione peroxidase (GSH-Px) were observed while the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GSH-Rd) were unchanged. In conclusion, findings from this study indicate that in vitro toxicity of bentazone may be associated with oxidative stress and this work warrants further in vivo investigations

Investigation on the toxic potential of Tribulus terrestris in vitro.

Context: Tribulus terrestris L. (Zygophyllaceae) has been commonly used to energize, vitalize, and improve sexual function and physical performance in men

In vitro antioxidant and cytotoxic activity of muscari neglectum growing in Turkey

Muscari species have been used as antirheumatic, stomachic, diuretic, expectorant and anti verruca in folk medicine. The species have also been used as food for humans and animals in Turkey. In this study, the antioxidant and cytotoxic activities of different extracts obtained from Muscari neglectum were studied. The antioxidant capacities of the extracts were evaluated with DPPH free radical scavenging activity, metal chelating capacity and ABTS cation radical scavenging capacity assays. The cytotoxic potentials of the extracts were determined by MTT and LDH assays on HeLa-cells (human cancer cell line) and NRK-52E-cells (rat kidney cell line). The aqueous extracts from M. neglectum bulbs (IC50: 2.83± 0.54 mg/mL) and herba (IC50: 8.52± 1.3 mg/mL) exhibited stronger DPPH free radical scavenging activity than other extracts, respectively. The ethanol extract from M. neglectum bulbs showed the highest ABTS (27.88 %) cation radical scavenging activity. According to the metal chelating activity test results, the aqueous extracts from M. neglectum herba and bulbs showed the strongest metal chelating activity (28.99% and 28.07%, respectively). © 2017 Marmara University Press, All Rights Reserved

In vitro antioxidant and cytotoxic activity of muscari neglectum growing in Turkey

Muscari species have been used as antirheumatic, stomachic, diuretic, expectorant and anti verruca in folk medicine. The species have also been used as food for humans and animals in Turkey. In this study, the antioxidant and cytotoxic activities of different extracts obtained from Muscari neglectum were studied. The antioxidant capacities of the extracts were evaluated with DPPH free radical scavenging activity, metal chelating capacity and ABTS cation radical scavenging capacity assays. The cytotoxic potentials of the extracts were determined by MTT and LDH assays on HeLa-cells (human cancer cell line) and NRK-52E-cells (rat kidney cell line). The aqueous extracts from M. neglectum bulbs (IC50 : 2.83 +/- 0.54 mg/mL) and herba (IC 50 : 8.52 +/- 1.3 mg/mL) exhibited stronger DPPH free radical scavenging activity than other extracts, respectively. The ethanol extract from M. neglectum bulbs showed the highest ABTS (27.88 %) cation radical scavenging activity. According to the metal chelating activity test results, the aqueous extracts from M. neglectum herba and bulbs showed the strongest metal chelating activity (28.99% and 28.07%, respectively)

AN EVALUATION OF ANTIOXIDANT, ANTIMICROBIAL, ANTIBIOFILM AND CYTOTOXIC ACTIVITIES OF FIVE VERBASCUM SPECIES IN TURKEY

Verbascum L. species are used for different medicinal purposes in Turkish folk medicine such as diuretic, expectorant, laxative, mucolytic, sedative, sudorific, and wound healer. Five Verbascum species, two of which are used in medicine, were investigated for their antimicrobial, antioxidant and cytotoxic activities. Determination of the total phenolic compounds, DPPH and ABTS methods were preferred for the antioxidant tests. The antimicrobial and antibiofilm activities of the extracts, which were not studied previously, were investigated against pathogenic and potential pathogenic agents. The cytotoxicity was assessed by MTT and LDH tests. This study is very important for finding new therapeutic sources and also new, natural sources for food preservation

Effects of bentazone on lipid peroxidation and antioxidant systems in human erythrocytes in vitro

Bentazone, a benzothiadiazole herbicide, is widely used for a variety of crops including cereals, maize, peas, rice and soy beans. The concern for human health is stil very high because bentazone is continuously monitored in environment and several studies to evaluate its potential carcinogenic effects when chronic and high doses were administered to animals. We aimed to investigate the possible effects of bentazone on lipid peroxidation, levels of glutathione and activities of antioxidant enzymes in human erythrocytes in vitro. For that, erythrocyte were incubated with bentazone in different concentrations (0-50 nM) at 37 degrees C for 1 hr. Bentazone showed significant increase in the levels of malondialdehyde (MDA) at the highest concentration in erythrocytes as an index of lipid peroxidation. Besides, alterations in the levels of reduced glutathione (GSH) and activities of glutathione peroxidase (GSH-Px) were observed while the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GSH-Rd) were unchanged. In conclusion, findings from this study indicate that in vitro toxicity of bentazone may be associated with oxidative stress and this work warrants further in vivo investigations