Labyrinthine fístulae in chronic otitis media with cholesteatoma

The chronic otitis media with cholesteatoma (COMC) may evoluate to intracranial and extra cranial complications, including the labyrithine fístulae. In this study, we present the evolution of our patients with labyrinthine fístulae. STUDY DESIGN: Clinical prospective. MATERIAL AND METHOD: Ten out 82 patients with COMC had labyrinthine fístulae and underwent surgery from January/2001 to April/2002. They were assessed by clinical exam, computed tomography scans, and pre and postoperative audiogram. RESULTS: Hearing loss, otorrhea, tinnitus and dizziness were present in 100%, 90%,80%, and 40% of the cases. In one patient the fístulae was seen only in the coronal CT-scan, in another patient the fístulae was not seen neither in coronal nor axial images. Among the patients who had tinnitus, 66% referred improvement of this complaint after surgery. DISCUSSION: in the cases without invasion of the perilymphatic space, we noticed a tendency of improvement of the postoperative audiogram pattern and clinical outcome. In the extensive fístulae, on the other hand, there were no clinical changes. CONCLUSION: The CT-scan remains the best exam to assess the COMC with 90% of sensitivity for labyrinthine fístulae. In the stage II we had a good postoperative outcome.A otite média crônica colesteatomatosa (OMCC) pode cursar com complicações intra e/ou extracranianas, entre elas a fístula labiríntica. Neste trabalho, mostramos a incidência e a evolução dos casos de fístula labiríntica decorrentes da OMCC em nosso serviço. FORMA DE ESTUDO: Clínico prospectivo. MATERIAL E MÉTODO: Dez pacientes com fístula labiríntica, do total de 82 pacientes com OMCC, foram submetidos à cirurgia no período de janeiro de 2001 a abril de 2002 e avaliados através de exame otorrinolaringológico completo, tomografia computadorizada e audiometria pré e pós-operatória. RESULTADOS: Perda auditiva, otorréia, zumbido e vertigem estavam presentes em 100%, 90%, 80% e 40% dos casos respectivamente na avaliação clínica pré-operatória. Em um paciente a fístula aparecia apenas nos cortes tomográficos coronais e tivemos um caso de falso-negativo. Dos pacientes com zumbido, 66% apresentaram melhora deste quadro no pós-operatório. DISCUSSÃO: Nos casos de fístula sem invasão do espaço perilinfático (até grau II), notamos uma tendência de melhora dos quadros clínico e audiométrico após a cirurgia. Nas fístulas extensas, por outro lado, o resultado audiométrico se manteve inalterado. CONCLUSÃO: A tomografia continua sendo o exame de escolha para os quadros de OMCC com sensibilidade de 90% para fístulas labirínticas. Nas fístulas grau II a cirurgia apresenta bom resultado funcional.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Otorrinolaringologia e Distúrbios da Comunicação HumanaUNIFESP, EPM, Depto. de Otorrinolaringologia e Distúrbios da Comunicação HumanaSciEL

Activator Protein-1 Transcriptional Activity Drives Soluble Micrograft-Mediated Cell Migration and Promotes the Matrix Remodeling Machinery

Impaired wound healing and tissue regeneration have severe consequences on the patient's quality of life. Micrograft therapies are emerging as promising and affordable alternatives to improve skin regeneration by enhancing the endogenous wound repair processes. However, the molecular mechanisms underpinning the beneficial effects of the micrograft treatments remain largely unknown. In this study, we identified the active protein-1 (AP-1) member Fos-related antigen-1 (Fra-1) to play a central role in the extracellular signal-regulated kinase- (ERK-) mediated enhanced cell migratory capacity of soluble micrograft-treated mouse adult fibroblasts and in the human keratinocyte cell model. Accordingly, we show that increased micrograft-dependent in vitro cell migration and matrix metalloprotease activity is abolished upon inhibition of AP-1. Furthermore, soluble micrograft treatment leads to increased expression and posttranslational phosphorylation of Fra-1 and c-Jun, resulting in the upregulation of wound healing-associated genes mainly involved in the regulation of cell migration. Collectively, our work provides insights into the molecular mechanisms behind the cell-free micrograft treatment, which might contribute to future advances in wound repair therapies

Simulations of economic results with the use of FTAI in cow-calf farm in the Pantanal.

The objective of the study was to evaluate, by system simulations, the cost-benefit of FTAI use in three different scenarios in the Pantanal of Mato Grosso do Sul: Conventional Ranch (P1), Conventional Ranch with FTAI (P2), and Ranch with cultivated pasture and the use of FTAI (P3), all based on modal farms in the Pantanal of Mato Grosso do Sul. The study simulated the impact of increased productivity and herd quality in the economic turnover of extensive breeding farms in the region. A deterministic model was developed, by using spreadsheets to perform the costs and the turnover calculations. The economic margins were estimated by the difference between the costs and turnovers of the production systems studied. The variables number of male and female calves weaned and increased number of cows in production showed the highest impact in the compensation. The use of FTAI on farms with natural or cultivated pastures increased the pregnancy rate at the end of the breeding season (EM), leading to an increased number of calves available for sale and consequently increased turnover and decreased marginal cost of the calf produced on extensive breeding farms in the Pantanal of Mato Grosso do Sul.Na publicação: Juliana Corrêa Borges

Embryos produced in vitro with sexed semen - technique application for superior beef calves production.

Due the relevance of the Zebu and the increasing use of production in vitro (PIV) of embryo in Brazil, we evaluated the pregnancy rates of Nelore recipients inovulated with fresh or vitrified IVP embryos obtained from Nelore donor oocytes from slaughterhouse (SLAUGHTER, n = 66 embryos) or OPU in vivo (VIVO, n = 299 embryos).Proceedings of the 30th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, PR, Brazil, August 25th to 27th, 2016, and 32nd Meeting of the European Embryo Transfer Association (AETE); Barcelona, Spain, September 9th and 10th, 2016. A040. Título em português: Utilização de embriões produzidos in vitro com sêmen sexado ? aplicação da técnica para produção de bezerros de corte superiores. Na publicação: Juliana Correa Borges

Integrating remote sensing, geographic information system and fuzzy logic: an index to evaluate the natural potential for livestock ranching in the Pantanal.

Abstract: In this study, an index natural potential for livestock ranching (NPLR) at ranch level in the Pantanal wetland was obtained using landscape indicators combined with fuzzy inference process. Four landscape indicators related with livestock production were selected by an expertise group. The application of this approach was illustrated through a pilot ranch located in the Nhecolândia sub-region, Pantanal, MS. Remote Sensing (RS) and Geographic Information System (GIS) technologies were used to map the vegetation types and aquatic habitats (landscapes types) in this ranch. Landscape type?s composition metrics were obtained using the ArcGis 9 and were used to estimate the four indicators selected by expertise: forest cover proportion (FC); landscape productive value (LPV); diversity of aquatic habitats (DAH) and flooding degree (FD). Fuzzy inference process involving definitions of membership functions, fuzzy set operations and inference rule were run and validated with the participation of core stakeholders. Different scenarios also were simulated in batch and validated with the participation of stakeholders. Both procedures were performed by Webfuzzy software. The NPLR index value found in the pilot ranch was as expected by both expertise and stakeholders. Fuzzy logic combined with landscape metric seems to be suitable for the definition of the natural potential of ranches to produce livestock in the Pantanal.Geopantanal 2012

Efficiency of post FTAI manegement in lacting beef cows.

The study aimed evaluate the effectiveness of TAI post reproductive management in lacting beef cows. Were inseminated at fixed time, 607 Nellore multiparous with 45 days postpartum, calved in November and inseminated with 5 Angus bulls in January 2015 in MS state farm.Proceedings of the 30th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, PR, Brazil, August 25th to 27th, 2016, and 32nd Meeting of the European Embryo Transfer Association (AETE); Barcelona, Spain, September 9th and 10th, 2016. A078. Título em português: Eficiência das técnicas de manejo reprodutivo pós-inseminação artificial em tempo fixo (IATF) em vacas de corte paridas. Na publicação: Juliana Correa Borges

Expressão de genes homólogos de Arabidopsis em arroz relacionados ao aumento do potencial produtivo.

O objetivo desse trabalho foi quantificar a expressão de genes de arroz homólogos de Arabidopsis previamente relacionados ao aumento do potencial produtivo (RUBIRICE, RUBICORN, AVP1, DA1 e TOR) por meio da análise de PCR quantitativo (qPCR). Os genótipos estudados nesse estudo foram BRSMG Curinga, Primavera e Douradão, os quais foram avaliados em um ensaio em casa de vegetação, cultivados sob dois níveis de fertilidade do solo (alta e baixa), em esquema fatorial, no delineamento inteiramente casualizado e com duas repetições

Repasse de IATF com monta natural nas proporções touro vaca de 1:20 e 1:40 alternado.

O Brasil apresenta amplo potencial para a pecuária e destaca-se no cenário mundial como o maior exportador de carne bovina. Detém o rebanho de 212 milhões de bovinos, dos quais participam do sistema de cria 90 milhões de matrizes, constituídas por vacas e novilhas adultas, e 2,50 milhões de touros. No País, mesmo com o crescente aumento da IATF, a taxa ainda é baixa, calculada em 12%, com base no número de doses comercializadas em 2016. Deduz-se, portanto, que seguramente mais de 80% dos bezerros nascidos são oriundos de acasalamentos naturais. O manejo reprodutivo do rebanho de corte brasileiro caracteriza-se por sistemas extensivos, com estações de monta definidas nos meses de primavera ou verão, sendo que cerca de 95% do processo reprodutivo ainda ocorre por monta natural Este trabalho tem por objetivo comparar a relação touro:vaca após IATF, na proporção de 1:20 direto e 1:40 alternado, com descanso dos touros a cada 25 dias, permitindo assim avaliar se há condições de diminuição do volume de touros em fazendas que realizam IATF

Common bean cultivar BRS Ametista with large Carioca grains and disease resistance.

BRS Ametista is a common bean cultivar with Carioca grain and yields similar to cultivar Pérola, but with larger grain size, resistance to anthracnose and Fusarium wilt. It is recommended for 18 states in all regions of Brazil and can be planted on over 95% of the area used for common bean in the country