Detection of interleukin-6 and interleukin-8 in serum from women with recurrent spontaneous abortion

To investigate the role of IL-6 and IL-8 in the immune-regulatory mechanisms involved in the recurrent spontaneous abortion of the first trimester of pregnancy. Serum level of IL-6 and IL-8 were determined in 25 women of age (20-35) years who had a spontaneous abortion of unknown aetiology during the first trimester of pregnancy .They were compared with the corresponding levels of 20 pregnant and non-pregnant women as control groups .cytokine levels were measured by (ELISA) technique .The women with spontaneous abortion had highly significant (P < 0.01) increased serum level of IL-8 and highly significant (P < 0.01 ) decreased level of IL-6 compared to those with normal pregnant and non-pregnant women. The results of this study may be related to the underlying aetiopathogenetic mechanisms , however , There is sufficient evidence for their use as predictive markers of pregnancy outcome

Assessment of Serum Prolactin Level in Patients Women with Rheumatoid Arthritis

The prolactin hormone played role in the many autoimmune disorders. To determine the importance of high levels of prolactin in triggering rheumatoid arthritis, thirty patient's women with hyperprolactinemia aged (20-45) years old have been investigated and compared with twenty five healthy individuals. All the studied groups were carried out to measure the concentration of citrulinated peptide(CCP) by enzyme linked immunosorbent assay( ELISA), antikeratin antibodies (AKA)and antinuclear antibodies(ANA) by indirect fluorescent assay IFAT. There was a significant elevation of CCP concentration compared with control groups (P< 0.05). The percentage of antikeratin antibodies and antinuclear antibodies was (20%, 10%) respectively, and there were significant differences (P< 0.05) between incidences percentage of antikeratin and antinuclear antibodies compared with control groups . This study indicated that women with rheumatoid arthritis may play a role as triggering factor of hyperprolactinemi

Heavy metals accumulation in macrophytes in the lakes of Bangalore urban

This study was mainly focused upon the impact of heavy metals pollution (Cd, Co, Cr, Cu, Mn, Ni, Pb and Zn) in aquatic macrophytes of the littoral zones of the lakes of Bangalore Urban region. The heavy metals concns. were as follows: Manganese (257.6 ± 39.6) ppm; Lead (66 ± 12.5)​ppm; Chromium (64.9 ± 6.2)​; Nickel (42.3 ± 3.2)​, Cobalt (18.8 ± 4.0) and Cadmium (4.7 ± 0.7)​. Copper, Manganese and Zinc were largely within normal range while Chromium, Lead, Cobalt and Nickel were in crit. range. There were more statistically significant correlations between metals in the dry than in the west sea..

Impact assessment of heavy metals pollution of Vartur lake, Bangalore

The unscientific disposal of wastes and pollution in urban water bodies has caused immense problems not only to human beings but also to the aquatic biodiversity. Assessment of heavy metals in Vartur Lake, Bangalore, revealed that Cadmium (Cd), Cobalt (Co), Chromium (Cr), Nickel (Ni), and Lead (Pb) all exceeded drinking water standards. Nevertheless, Chromium (Cr) exceeded the CPCB’s tolerance limits for water bodies subjected to effluent discharge. In Eichornia crassipes, Cd, Co, Cr, and Ni were critical. In the sediments, Cadmium (Cd) and Nickel (Ni) exceeded the Probable Effect Level (PEL). Sediment Geo-accumulation Index of the lake showed moderate contamination with Mn, Cu, and Pb. Cr had the highest plant/sediments metal concentration factor even though its bioavailability in plants (71.5 ppm) was lower compared to Manganese (192.3 ppm). This calls for immediate action to be implemented to carry out necessary environmental mitigation measures for the lake

Knowledge, Attitudes and Practices on HIV Prevention\ud Among Secondary School Students in Bukoba Rural,\ud Kagera Region- Tanzania

It is estimated that about 42 million people are living with HIV/AIDS worldwide. Among them 90% are from developing countries and more than 70% of them are found in Sub-Saharan Africa including Tanzania. About 4.8 million people are infected annually, 70% being women and 3 million are in Sub-Saharan Africa. Approximately 3 million people died from AIDS in 2003 worldwide. Documented data show that the reproductive age group is more vulnerable and thus an intervention is necessary in this age group. To assess knowledge, attitudes and practices on HIV preventive measures among secondary school students. Bukoba district, Kagera region, Tanzania A cross sectional study was conducted in Bukoba rural district, Kagera region, in September 2005. Multi-stage sampling technique was used to determine the study population and 364 students were interviewed using structured questionnaire. Our study findings showed that 93.7% of students knew how HIV is transmitted and 86.6% knew at least one method of HIV prevention. Students mentioned abstinence and faithfulness to one partner as best methods for HIV prevention. Despite the knowledge they have, very few students reported to have used condoms in their last sexual contacts. Radio was reported to be the major source of information even though information given was not satisfactory to most of the students. In addition, 50% of students reported to have experienced sex, the peak age of first sexual intercourse being 15 years. Most of the students had an objective knowledge on HIV/AIDS transmission and prevention though their attitudes and practices were differen

Bacteria isolated from bloodstream infections at a tertiary hospital in Dar es Salaam, Tanzania – antimicrobial resistance of isolates

Objective. A bloodstream infection (BSI) is a life-threatening condition. We studied the causative agents of BSIs and antimicrobial susceptibility patterns of bacterial isolates at Muhimbili National Hospital (MNH) in Dar es Salaam, Tanzania. Methods. A retrospective analysis of blood culture results obtained at MNH from January 2005 to December 2009 was done. Blood culture isolates judged to be clinically significant and antimicrobial susceptibility results of the bacteria were included. The frequencies and proportions of bacteria isolated and antimicrobial susceptibility results were analysed and compared using Pearson’s chi-square test and Fisher’s exact test where applicable, or the Mann-Whitney U-test. Results. A total of 13 833 blood cultures were performed. Bacterial pathogens were detected in 1 855 (13.4%), Gram-positive bacteria (1 523; 82.1%) being significantly more prevalent than Gramnegative bacteria (332; 17.9%) (p=0.008). The most common bacterial pathogens isolated were coagulase-negative staphylococci (CoNS) (1 250; 67.4%), S. aureus (245; 13.2%), Escherichia coli (131; 7%) and Klebsiella spp. (130; 7.0%). All bacteria isolated showed high resistance to penicillin G (70.6%), tetracycline (63.8%), cefotaxime (62.5%) and ampicillin (62.3%). Moderate to high resistance was seen against chloramphenicol (45.2%), erythromycin (35.0%), ciprofloxacin (29.3%), co-trimoxazole (25.0%) and gentamicin (23.5%). Of S. aureus isolates, 23.3% were resistant to methicillin. Conclusions. CoNS accounted for two-thirds of the bacterial pathogens isolated. High-level resistance was seen to first-line and inexpensive antimicrobial agents. Routine screening for extendedspectrum beta-lactamase production and methicillin resistance among Gram-negative rods and S. aureus from blood cultures should be instituted to monitor spread of multidrug-resistant isolates

Detection of Type 2 Diabetes Mellitus in Serum from Women with Polycystic Ovarian Syndrome

Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorder. To determine the metabolic disorders in women with PCOS, (25) women with PCOS ages (15 - 47) years have been investigated and compared with (20) healthy individuals. All the studied groups were carried out to measure fasting blood sugar, (anti-GAD Ab, anti ?-islet cell Ab by IFAT) and measured insulin level by ELISA. There was significant elevation in the concentration of fasting blood sugar than in control groups (p ? 0.05) and there was negative results for anti-GAD Ab and anti ?-islet cell Ab by IFAT test for serum of women with PCOS, while there was significant differences in the insulin level for women with PCOS compared with control groups (p ? 0.05), these indicated that women with PCOS are at high risk for type -2 Diabetes mellitus and reflecting insulin resistance

Evaluation of HIV antigen /antibody combination ELISAs for diagnosis of HIV infection in Dar Es Salaam, Tanzania

Introduction: the aim of this study was to evaluate the performance of Enzygnost HIV Integral II antigen/antibody combination ELISAs in orderto formulate HIV ELISA testing algorithms for the Ministry of Health and Social Welfare, Tanzania. Methods: this was a laboratory-based evaluation of Enzygnost HIV Integral II Antibody/ Antigen, Murex HIV antigen/antibody and Vironostika HIV Uniform II antigen/antibody conducted between October 2011 and May 2012. Results: a total of 600 blood samples were included in the evaluation. A total of 209/596 (35.1%) serum samples were confirmed HIV positive. Of these, the prevalence of HIV infection was 2.3% (3/130), 2.3% (3/127), 2.2% (3/139) and 100% (200/200) for VCT clients, ANC attendees, blood donors and CTC patients, respectively. Three hundred and eighty seven (64.9%) were HIV negative samples. Sensitivity was 100% (95% CI; 98.3-100%) for all the three HIV ELISAs. The specificity for the Enzygnost HIV Integral II and Murex was 100% (95% CI; 99.1-100%). The final specificity at repeat testing was 99.5% (95% CI; 98.2-99.9%) for  Vironostika. Enzygnost HIV Integral II detected HIV infection seven days since first bleed.Conclusion: initial testing using either Vironostika or Murex HIV   antigen/antibody combination ELISA followed by testing of reactive samples on the Enzygnost HIV Integral II gave a sensitivity and specificity of 100% with reduced window period. Combination of two HIV antigen/antibody  combination ELISAs can be used as an alternative confirmatory testing  strategy for screening of donated blood at the National and Zonal blood transfusion centres and in lab diagnosis of HIV infection

Chemical Polymerization Kinetics of Poly-O-Phenylenediamine and Characterization of the Obtained Polymer in Aqueous Hydrochloric Acid Solution Using K 2

The oxidative chemical polymerization of o-phenylenediamine (OPDA) was studied in hydrochloric acid solution using potassium dichromate as oxidant at 5°C. The effects of potassium dichromate, hydrochloric acid, and monomer concentrations on the polymerization reaction were investigated. The order of reaction with respect to potassium dichromate, hydrochloric acid, and monomer concentration was found to be 1.011, 0.954, and 1.045, respectively. Also, the effect of temperature on the polymerization rate was studied and the apparent activation energy of the polymerization reaction was found to be 63.658 kJ/mol. The obtained polymer was characterized using XPS, IR, UV-visible, and elemental analysis. The surface morphology of the obtained polymers was characterized by X-ray diffraction and transmission electron microscopy (TEM). The TGA analysis was used to confirm the proposed structure and number of water molecules in each polymeric chain unit. The ac conductivity (σac) of (POPDA) was investigated as a function of frequency and temperature. The ac conductivity was interpreted as a power law of frequency. The frequency exponent (s) was found to be less than unity and decreased with the increase of temperature, which confirms that the correlated barrier hopping model was the dominant charge transport mechanism