Effects of benzylaminopurine and naphthalene acetic acid on proliferation and shoot growth of pineapple (Ananas comosus L. Merr) in vitro

This study was conducted to evaluate the pineapple regeneration and shoot growth as affected by 6- benzylaminopurine (BAP) at 2.0 mg/l and naphthalene acetic acid (NAA) at 0.2 mg/l in vitro. BAP and NAA at the concentration of 2.0 and 0.2 mg/l were used in this study. BAP at 2.0 mg/l significantly affected the production of shoots per explant, shoot length and weight. Total shoot length was higher in BAP (2 mg/l) than in control (MS medium without hormone) and NAA (0.2mg/l) after 10, 20, 30, 40, 50 and 60 days incubation period. Total shoot length was highest in BAP in all incubation periods. Total shoot weight was higher in BAP (2 mg/l) and lower in NAA (0.2 mg/l) as compared to MS medium without hormone. The results showed that BAP at the concentration of 2 mg/l was effective for pineapple shoot growth and development.Key words: Pineapple regeneration, shoot growth, 6-benzylaminopurine, naphthalene acetic acid

Physiochemical and Phytochemical Properties of Wax Apple (Syzygium samarangense [Blume] Merrill & L. M. Perry var. Jambu Madu) as Affected by Growth Regulator Application

This study represents the first paper of the effects of growth regulators on the physiochemical and phytochemical properties of the wax apple fruit, a widely cultivated fruit tree in southeast Asia. Net photosynthesis, sucrose phosphate synthase (SPS) activity, peel color, fruit firmness, juice content, pH value, total soluble solids (TSSs), and the sugar acid ratio were all significantly increased in growth regulators (PGRs) treated fruits. The application of gibberellin (GA3), naphthalene acetic acid (NAA), and 2,4-dichlorophenoxy acetic acid (2,4-D) significantly reduced titratable acidity and increased total sugar and carbohydrate content compared to the control. The 50 mg/L GA3, 10 mg/L NAA, and 5 mg/L 2,4-D treatments produced the greatest increases in phenol and flavonoid content; vitamin C content was also higher for these treatments. PGR treatment significantly affected chlorophyll, anthocyanin, and carotene content and produced higher phenylalanine ammonia lyase (PAL) and antioxidant activity levels. There was a positive correlation between peel color and TSS and antioxidant activity and both phenol and flavonoid content and PAL activity and anthocyanin formation. A taste panel assessment was also performed, and the highest scores were given to fruits that had been treated with GA3 or auxin. The study showed that application of 50 mg/L GA3, 10 mg/L NAA, and 5 mg/L 2,4-D once a week from bud development to fruit maturation increased the physiochemical and phytochemical properties of wax apple fruits

Effects of rice husk biochar on nutrient uptake and yield ofleafy vegetables: a review

Green leafy vegetables have been used since ancient times as a food source because they contain many nutrients and minerals that help for growth and maintaining human health. The objective of this study is to sumarize the effect of rice husk biochar on the selected leafy vegetables. The selected leafy vegetables are spinach (Spinacia oleracea L.), cabbage (Brassica chinensis L.), lettuce (Lactuca sativa L.), Chinese cabbage (Brassica rapa chinensis),Amaranth (Amaranthus viridis L.). Day by day the demand of leafy vegetables is increasing due to consume by many people. To fulfill this demand, the production of leafy vegetables should be increased. Various organic amendments such as rice husk biochar (RHB) can be used to increase the yield. RHB is one of the soil amendments that contain nutrients which is importantforsoil health and crop production. Although characteristics of RHB are also related to other factors such as pyrolysis heating rate and residence time, its performance for specific applications (e.g. carbon sequestration, pH amendment) can be manipulated through adjustingthe pyrolysis temperature. Recent studies show the success of multi-application of biochar thataffect the soil chemical and physical properties, plant growth and yield of vegetables. This review summarizes nutrient uptake and yield of some leafy vegetables which were influenced by rice husk biochar

Effects of the phloemic stress on the growth, development and quality of wax apple (Syzygium samarangense) cv. Jambu madu

A study was carried out to investigate the effects of different girdling techniques on the growth, development and quality of red wax apple fruits (Syzygium samarangense). Selected horticultural parameters were monitored at one week interval during the growth period from December 2008 to October 2010, using I, C, V shaped, 50%, and 100% girdling. Girdling was applied 2 weeks before flowering. The results showed that the C-shaped girdling technique produced the best results with regard to the fruit setting and chlorophyll content in comparison to the control and the other girdling techniques employed. Furthermore, C-shaped girdling enhanced faster fruit growth producing the best final fruit length and diameter. In addition to significantly increased yield, juice percentage, leaf dry matter, biomass and TSS content of fruits. It was also observed that 50% girdling decreased fruit dropping. V-shaped girdling also increased the number of bud and reduced bud dropping compared to control. I-shaped girdle fruits produced the highest amount of K+ content in fruit juice compared to other treatments. The color development of the fruit peel in 100% girdling was also the highest among the treatments. From this study, it can be concluded that C-shaped girdling applied two weeks before flowering produced better fruit growth, yield and quality of red wax apple fruits under field conditions

GROWTH, YIELD AND POSTHARVEST QUALITY OF WAX APPLE AS AFFECTED BY NAPHTHALENE ACETIC ACID APPLICATION

Results of this study represent the first report of the effect of Naphthalene Acetic Acid (NAA) on the pre and post harvest quality of wax apple fruit. The wax apple trees were spray treated with 0, 5, 10 and 20 mg L-1 NAA under field conditions during 2008 to 2011. The experiments were carried out in Completely Randomized Design (CRD) with six replications. Leaf chlorophyll content, chlorophyll fluorescence, photosynthetic yield, net photosynthetic rate, drymatter content of leaves and total soluble solids and K+content of wax apple fruits were significantly increased after treatments with 10 mg L-1. Polygalacturonase activity significantly decreased with NAA treatments. The application of 5 mg L-1 NAA increased 27% more bud and reduced 42% less fruit drop compared to the control. In addition, higher protein and phosphate synthase activity of leaves, fruit set, fruit growth, larger fruit size and yield were recorded in NAA treated plants. In storage, treated fruits exhibited higher TSS and firmness and less weight loss, browning, titratable acidity, respiration and ethylene production than the control. It is concluded that spraying with 5 and 10 mg L-1 NAA once a week under field conditions produced better fruit growth and yield of the wax apple and maintained better fruit quality in postharvest storage