Adaptive Optics Feedback Control

This book is a collection of 19 articles which reflect the courses given at the Collège de France/Summer school “Reconstruction d'images − Applications astrophysiques“ held in Nice and Fréjus, France, from June 18 to 22, 2012. The articles presented in this volume address emerging concepts and methods that are useful in the complex process of improving our knowledge of the celestial objects, including Earth

Trapianto renale e infezione da HCV: analisi retrospettiva dei dati relativi alla popolazione del Centro Trapianti di Pavia

Abstract non disponibil

Isolation and phenotyping of potential stem cells from the umbilical cord of the bottlenose dolphin(Tursiops truncatus)

We have successfully isolated cells with stem-like properties from bottlenose dolphin (Tursiops truncatus) umbilical cord. Our results show that this cetacean species has embryonic fetal and adult stem cells as do humans and other studied mammals. This accomplishment allows to eventually investigate whether dolphins, due to their unique adaptations to aquatic environments, have special stem cell lineages or distinctive mechanisms of cell programming. Further characterization of their potency to differentiate into multiple cell lineages would fulfill numerous applicative purposes. We characterized, developed and refined a new protocol for obtaining potential stem cells from umbilical cord tissues of the bottlenose dolphin. Tissue samples were taken from umbilical cords of successful deliveries immediately after placenta ejection and collection from the water. Umbilical cord samples (2-3 cm3) were excised and subjected to enzymatic digestion and mechanical dissociation. Viable cells from specimens resident in the Oceanografic Valencia were cultured and subsequently isolated and tested for pluripotent characteristics (cell morphology, phenotype and expression of surface markers). Cell viability was confirmed also after freezing/thawing. The established protocol is suitable for collection/isolation/culture of dolphin potential mesenchymal stem cells from dolphin umbilical cord, which can be deposited in cell banks for future research needs

IDENTIFICAZIONE RAPIDA DI CEPPI DI CITOMEGALOVIRUS UMANO FARMACO-RESISTENTI MEDIANTE nPCR-RFLP IN SOGGETTI SOTTOPOSTI A TRAPIANTO.

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Clinical correlates of complicated grief among individuals with acute coronary syndromes

OBJECTIVE: The study aimed at exploring bereavement and complicated grief (CG) symptoms among subjects without a history of coronary heart disease (CHD) at the time of a first acute coronary syndrome (ACS) and to evaluate the relationship of CG symptoms and ACS. METHOD: Overall, 149 subjects with ACS (namely, acute myocardial infarct with or without ST-segment elevation or unstable angina), with no previous history of CHD, admitted to three cardiac intensive care units were included and evaluated by the Structured Clinical Interview for Complicated Grief (SCI-CG), Hamilton Depression Rating Scale, Hamilton Anxiety Rating Scale, and the 36-item Short-Form Health Survey (MOS-SF-36). RESULTS: Of the total sample of 149 subjects with ACS, 118 (79.2%) met criteria for DSM-5 persistent complex bereavement disorder. Among these, subjects who lost a partner, child, or sibling were older (P=0.008), less likely to be working (P=0.032), and more likely to be suffering from hypertension (P=0.021), returned higher scores on the SCI-CG (P=0.001) and developed the index ACS more frequently between 12 and 48 months after the death than those who lost a parent or another relative (P≤0.0001). The occurrence of ACS 12-48 months (P=0.019) after the loss was positively correlated with SCI-CG scores. An inverse relationship with SCI-CG scores was observed for patients who experienced ACS more than 48 months after the loss (P=0.005). The SCI-CG scores significantly predicted lower scores on the "general health" domain of MOS-SF-36 (P=0.030), as well as lower scores on "emotional well-being" domain (P=0.010). CONCLUSION: A great proportion of subjects with ACS report the loss of a loved one. Among these, the loss of a close relative and the severity of CG symptoms are associated with poorer health status. Our data corroborate previous data indicating a strong relationship between CG symptoms and severe cardiac problems

Trapianto renale e infezione da HCV: analisi retrospettiva dei dati relativi alla popolazione del Centro Trapianti di Pavia

Abstract non disponibil

Italy: The Archaeology of Palaeoshorelines, Coastal Caves and Seafaring Connections

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Effects on immunity of exposure to microplastics in adult zebrafish

It is now widely accepted that microplastics (MPs) represent a serious concern for aquatic environments, therefore assesment of biological pathways affected is crucially relevant. This study focused on variations of liver transcriptome, histology of gastrointestinal tract and gills, and locomotor activity of exposed fish along various days after treatment. Adult zebrafish (3 groups, N=12 each) were fed for 20 days with dry food alone (controls) or supplemented with a mix of pristine high-density polyethylene and polystyrene microplastics (0.1 or 1 mg/L), ranging in size from <25 to 90 μm. The exposure to MPs resulted in differential transcription of 324 genes in total, already affected at the lower dose, mainly involved in cholesterol biosynthesis (fatty acid degradation) and immunity pathways. Up-regulation of transcripts subserving response to extra-cellular antigens, and down- regulation of others involved in innate antimicrobial response, antiviral defense and maintenance of epithelial integrity highligted defective control of pathogen entry at epithelial barriers, confirmed by occurrence of histopathological signs in both intestine and gills. Furthermore, variations in energy utilization likely accounted also for alteration of circadian rhythm of locomotor activity

Immunodetection of IgM, IgT and pIgR in mucosal tissues of Antarctic teleost

We have previously investigated the immune response at hepato-biliary level in the Antarctic teleost Trematomus bermacchii, a species belonging to the Perciform suborder Notothenoidei, the most abundant component of the fish fauna living in the Antarctic ocean. By that time only the IgM isotype was known and well characterized at molecular and biochemical levels in Antarctic fish. Over the past few years we have cloned and sequenced genes encoding other two key molecules of the mucosal immune system, IgT and polymeric Ig receptor (pIgR) of T. bernacchii. The present study aimed at investigating the localization in mucosal tissues of IgM, IgT and pIgR in an attempt to clarify the protein occurrence and transepithelial transport. Biochemical and immunohistochemical data provided convergent data about specific mechanisms operating apical release of IgT in exocrine way, as well as depicting peculiar (maybe ancestral) features compared with well- known mechanisms described for polymeric Igs transport in mammalian tissues