376 research outputs found
T cell receptor (TCR)-induced death of immature CD4<SUP>+</SUP>CD8<SUP>+</SUP> thymocytes by two distinct mechanisms differing in their requirement for CD28 costimulation: implications for negative selection in the thymus
Negative selection is the process by which the developing lymphocyte receptor repertoire rids itself of autoreactive specificities. One mechanism of negative selection in developing T cells is the induction of apoptosis in immature CD4+CD8+ (DP) thymocytes, referred to as clonal deletion. Clonal deletion is necessarily T cell receptor (TCR) specific, but TCR signals alone are not lethal to purified DP thymocytes. Here, we identify two distinct mechanisms by which TCR-specific death of DP thymocytes can be induced. One mechanism requires simultaneous TCR and costimulatory signals initiated by CD28. The other mechanism is initiated by TCR signals in the absence of simultaneous costimulatory signals and is mediated by subsequent interaction with antigen-presenting cells. We propose that these mechanisms represent two distinct clonal deletion strategies that are differentially implemented during development depending on whether immature thymocytes encounter antigen in the thymic cortex or thymic medulla
A Detailed Observational Analysis of V1324 Sco, the Most Gamma-Ray Luminous Classical Nova to Date
It has recently been discovered that some, if not all, classical novae emit
GeV gamma rays during outburst, but the mechanisms involved in the production
of the gamma rays are still not well understood. We present here a
comprehensive multi-wavelength dataset---from radio to X-rays---for the most
gamma-ray luminous classical nova to-date, V1324 Sco. Using this dataset, we
show that V1324 Sco is a canonical dusty Fe-II type nova, with a maximum ejecta
velocity of 2600 km s and an ejecta mass of few
M. There is also evidence for complex shock interactions, including a
double-peaked radio light curve which shows high brightness temperatures at
early times. To explore why V1324~Sco was so gamma-ray luminous, we present a
model of the nova ejecta featuring strong internal shocks, and find that higher
gamma-ray luminosities result from higher ejecta velocities and/or mass-loss
rates. Comparison of V1324~Sco with other gamma-ray detected novae does not
show clear signatures of either, and we conclude that a larger sample of
similarly well-observed novae is needed to understand the origin and variation
of gamma rays in novae.Comment: 26 pages, 13 figure
Molecular and cellular characteristics of hybrid vigour in a commercial hybrid of Chinese cabbage
Abstract
Background
Heterosis or hybrid vigour is a phenomenon in which hybrid progeny exhibit superior performance compared to their parental inbred lines. Most commercial Chinese cabbage cultivars are F1 hybrids and their level of hybrid vigour is of critical importance and is a key selection criterion in the breeding system.
Results
We have characterized the heterotic phenotype of one F1 hybrid cultivar of Chinese cabbage and its parental lines from early- to late-developmental stages of the plants. Hybrid cotyledons are larger than those of the parents at 4 days after sowing and biomass in the hybrid, determined by the fresh weight of leaves, is greater than that of the larger parent line by approximately 20 % at 14 days after sowing. The final yield of the hybrid harvested at 63 days after sowing is 25 % greater than the yield of the better parent. The larger leaves of the hybrid are a consequence of increased cell size and number of the photosynthetic palisade mesophyll cells and other leaf cells. The accumulation of plant hormones in the F1 was within the range of the parental levels at both 2 and 10 days after sowing. Two days after sowing, the expression levels of chloroplast-targeted genes in the cotyledon cells were upregulated in the F1 hybrid relative to their mid parent values. Shutdown of chlorophyll biosynthesis in the cotyledon by norflurazon prevented the increased leaf area in the F1 hybrid.
Conclusions
In the cotyledons of F1 hybrids, chloroplast-targeted genes were upregulated at 2 days after sowing. The increased activity levels of this group of genes suggested that their differential transcription levels could be important for establishing early heterosis but the increased transcription levels were transient. Inhibition of the photosynthetic process in the cotyledon reduced heterosis in later seedling stages. These observations suggest early developmental events in the germinating seedling of the hybrid may be important for later developmental vigour and yield advantage.This work was supported in part by a grant-in-aid
for Scientific Research on Innovative Areas (24113509) (JSPS), by the Sasakawa
Scientific Research Grant (24â517) from The Japan Science Society, by Grant
for Promotion of Niigata University Research Projects (23C024) and by PREST
(12101066) (JST) to R. Fujimoto
A Planetary Microlensing Event with an Unusually Red Source Star: MOA-2011-BLG-291
We present the analysis of planetary microlensing event MOA-2011-BLG-291,
which has a mass ratio of and a source star that
is redder (or brighter) than the bulge main sequence. This event is located at
a low Galactic latitude in the survey area that is currently planned for NASA's
WFIRST exoplanet microlensing survey. This unusual color for a microlensed
source star implies that we cannot assume that the source star is in the
Galactic bulge. The favored interpretation is that the source star is a lower
main sequence star at a distance of kpc in the Galactic disk.
However, the source could also be a turn-off star on the far side of the bulge
or a sub-giant in the far side of the Galactic disk if it experiences
significantly more reddening than the bulge red clump stars. However, these
possibilities have only a small effect on our mass estimates for the host star
and planet. We find host star and planet masses of and from a Bayesian
analysis with a standard Galactic model under the assumption that the planet
hosting probability does not depend on the host mass or distance. However, if
we attempt to measure the host and planet masses with host star brightness
measurements from high angular resolution follow-up imaging, the implied masses
will be sensitive to the host star distance. The WFIRST exoplanet microlensing
survey is expected to use this method to determine the masses for many of the
planetary systems that it discovers, so this issue has important design
implications for the WFIRST exoplanet microlensing survey
Planet Sensitivity from Combined Ground- and Space-based Microlensing Observations
To move one step forward toward a Galactic distribution of planets, we
present the first planet sensitivity analysis for microlensing events with
simultaneous observations from space and the ground. We present this analysis
for two such events, OGLE-2014-BLG-0939 and OGLE-2014-BLG-0124, which both show
substantial planet sensitivity even though neither of them reached high
magnification. This suggests that an ensemble of low to moderate magnification
events can also yield significant planet sensitivity and therefore probability
to detect planets. The implications of our results to the ongoing and future
space-based microlensing experiments to measure the Galactic distribution of
planets are discussed.Comment: 10 pages, 5 figures, 1 table; ApJ in pres
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