Comparative performance of two extractants in colorimetric determination of soil organic matter in paddy soil

Commonly used soil organic carbon (SOC) determination methods are expensive, time consuming, require equipment and skilled personnel making them impractical to use at field level. Therefore, a laboratory analytical study was carried out to identify a suitable extractant for SOC extraction as part of an ongoing effort to develop a low cost quick method for determining soil organic matter (SOM) at field level. For this purpose, 38 soil samples were collected from different Agro Ecological Zone (AEZ) to represent typical rice based cropping pattern covering a sufficient range in texture, soil organic matter, pH, and type of management. Two extractants e.g. NaOH-Pyrophosphate + HCl and NaOH-EDTA were used to extract organic carbon from soil and two wavelength e.g. 300 and 400 nm were used to measure the absorbance of organic carbon in spectrophotometer. Soil organic carbon measurements by CNS analyzer were used as reference measurement to compare the extracting efficiency of SOC by the selected extractants. Both the extract and wavelength showed different degrees of error in OC measurement. The error sum square of NaOH-Pyrophosphate + HCl and NaOH-EDTA measured at 300 nm wavelength were 15.21 and 13.35 whereas at 400 nm it was 227.37 and 19.95, respectively. Consequently, the average error percentages at 300 nm were -33 and -8 while at 400 nm it was - 187 and -16. Based on the error sum square and error percentage, NaOH-Pyrophosphate + HCl performed better than the NaOH-EDTA, and 400 nm wavelength came out better than the 300 nm wavelength. In addition, both the extracts produced coloured solutions which were easily visible or detectable by naked eye and the colour intensity increased with the increase in SOC content. Variations in colour among the soils depending on organic matter content indicate that it would be possible to develop a new low cost method for SOM determination at field level by using NaOH-Pyrophosphate + HCl as the extractant. Further research is warranted to confirm this finding

Development of in vitro propagation system for Atriplex halimus L.

Explants excised from adult shrubs were surface sterilized and cultured on Murashige and Skoog (MS) basal medium in thepresence of plant growth regulators (PGRs) at different concentrations. A high multiplication rate of 7.2-fold was achieved every four weekson MS medium supplemented with 4.44 μM BA, 0.49 μM IBA and 0.58 μM GA3. Rooting was achieved with 73% efficiency within 2-4 weekson agar-gelled MS basal medium free of PGRs. Rooted plantlets were gradually acclimatized to field conditions over 5-6 weeks with 65%efficiency. For in vitro selection for salt tolerance, MS medium was supplemented with increasing concentrations of NaCl ranging between25 and 1000 mM. This study has demonstrated that in vitro shoots could tolerate up to 600 mM NaCl with optimal growth at 200 mM, whilehigher concentrations of NaCl affected growth negatively. Growth and shoot number decreased with increasing NaCl concentration with allplantlets died at 1000 mM NaCl

MALE FACTOR Inhibition of human sperm respiration by 4-hydroperoxycyclophosphamide and protection by mesna and WR-1065

Objective: To determine the effect of 4-hydroperoxycyclophosphamide (4OOH-CP) on the respiration of human sperm, and investigate the protective properties of mesna and WR-1065. Setting: SUNY Upstate Medical University, Syracuse, NY. Patient(s): Men (n ¼ 12) visited the Andrology Department for fertility evaluation. Intervention(s): None. Main Outcome Measure(s): Sperm respiration. Result(s): Immediate decline in the rate of respiration was observed when 4OOH-CP was added to washed sperm or semen. The inhibition was concentration dependent. The respiration was less affected when 4OOH-CP was added to semen, suggesting the presence of protective factors in the seminal plasma. Excess of mesna or WR-1065 ameliorated the effect of 4OOH-CP. Mesna was the more potent of the two compounds. 4OOH-CP also inhibited the respiration of mitochondria from beef heart. Conclusion(s): These findings emphasize the adverse effects of alkylating agents on sperm function. The results also provide a framework for thiol drug administration with high-dose alkylating agents to protect male fertility. The protective capacity of seminal plasma deserves further testing. (Fertil Steril Ò 2009;91:173-8. Ó2009 by American Society for Reproductive Medicine.

The Effect of Neutrophil-lymphocyte Ratio on 10-year Survival Outcomes Following Elective Open and EVAR Procedures

Objectives: The neutrophil-lymphocyte ratio (NLR) is a useful and inexpensive inflammatory marker associated with surgical outcomes. This study evaluates the effects of NLR on survival after elective endovascular (EVAR) and open aortic repair (OAR) of abdominal aortic aneurysm. Methods: We retrospectively reviewed patients from 1989 to 2019 who underwent elective OAR or EVAR at two separate academic centers. Baseline comorbidities were assessed. A receiver operating characteristic (ROC) curve was used to determine a cutoff point where NLR was associated with outcome. Kaplan-Meier survival analysis was used to compare survival through 10-year follow-up. Results: Overall, 437 patients (mean age, 72.0 6 10.1 years; 74.1% male) underwent 213 EVARs and 224 OARs. Median duration of follow-up was 4.55 years. The analysis of the ROC curve yielded an NLR of 3.94 with the highest specificity and sensitivity for 10-year survival. Baseline characteristics were similar between groups, except for an increased age in the group with NLR \u3e3.94 (73.5 vs 70.9 years; P ¼ .008) (Table). KaplanMeier analysis revealed that patients with NLR \u3e3.94 had decreased 10-year survival (37.2% vs 54.2%; P ¼ .0001) (Fig). By univariate analysis, NLR \u3e3.94 (P ¼ .0001), chronic obstructive pulmonary disease (P ¼ .006), and increased age (P ¼ .0001) were associated with increased mortality. On multivariable cox regression analysis, an NLR \u3e3.94 (odds ratio [OR], 1.69; 95% confidence interval [CI], 1.19-2.40), increased age (OR, 1.05; 95% CI, 1.03-1.07), and chronic obstructive pulmonary disease (OR, 1.44; 95% CI, 1.01-2.07) were associated with increased risk of mortality. Between OAR and EVAR, no difference in late survival was noted (49.9% vs 43.5%; P ¼ .24). Conclusions: An NLR \u3e3.94 is associated with increased mortality over a 10-year follow-up period after open and endovascular aortic repair. Future studies to further understand the driving force between an elevated NLR and increased mortality are warranted

Effect of long term fertilization on soil respiration and enzyme activities in floodplain soil

In agricultural farming system organic manuring and inorganic fertilizer application are the most common agricultural practices. Different fertilizer application lead to differences in soil nutrients, pH, and microbial species, which in turn affect the transformation and decomposition of organic carbon by soil microorganisms. Therefore, a laboratory incubation study was carried out to investigate the influence of long term manuring and fertilization on soil respiration by means of C mineralization and enzyme activities. A parallel first- and zero-order kinetic model was used to describe observed C mineralization in soil. The annual carbon mineralization was found to be significantly influenced by different fertilizer. This result indicates that more stable organic matter was formed in NP treated soil which is less prone to decomposition if present crop management has been changed. Other ward, NP has the highest potentiality to soil for the purpose of carbon sequestration in floodplain soil compared to other fertilizer. Urease activities varied from 4.7µg NH4-N/g soil/2h in NK treatment to 25.7µg NH4-N/g soil/2h in N+FYM treatment. N treatment had a significantly higher urease activity compared to the respective controls. When P, K, S and PK applied separately with N then the treatments show low enzyme activity to control and other treatments (N, N+FYM and NPKSZn). There were no significant differences for Arylamidase activities among the treatments. The arylamidase activities decreased when S applied in combination with N. On the other hand arylamidase activity increased with the application of all other treatment

Long - term fertilization effect of organic carbon and total nitrogen on floodplain soil

Soil organic matter is the most often reported indicator of soil quality and productivity and an evidence of previous soil management. Therefore, in 2017, a laboratory incubation study was carried out in the experimental filed of Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh, Bangladesh under control condition at 25°C for 104 days to investigate the influence of long term manuring and fertilization on soil respiration by means of C mineralization. Soil samples were collected from floodplain soil with rice-rice cropping pattern at Bangladesh Agricultural University (BAU) experimental farm having eight treatments. Long term (33 years) application of fertilizers and manure resulted in significant differences in soil organic carbon, total N content, and soil pH KCl between the treatments. The soil organic carbon and total N content varied among the different treatments from14.9 g OC kg-1 to 17.0 g OC kg-1 and1.60 g N kg-1 (control) to 1.78 g N kg-1 (application of NPK). The soil pH varied among the different treatments from 5.65(application of NK) to 4.89 (application of N). This result indicates that more stable organic carbon was formed in NPK treated soil which is less prone to de-composition if present crop management has been changed

Effect of Intubation Timing on the Outcome of Patients With Severe Respiratory Distress Secondary to COVID-19 Pneumonia.

Background: The optimal timing of intubation for critically ill patients with severe respiratory illness remains controversial among healthcare providers. The coronavirus disease 2019 (COVID-19) pandemic has raised even more questions about when to implement this life-saving therapy. While one group of providers prefers early intubation for patients with respiratory distress because these patients may deteriorate rapidly without it, other providers believe that intubation should be delayed or avoided because of its associated risks including worse outcomes. Research question: Our objective was to assess whether the timing of intubation in patients with severe COVID-19 pneumonia was associated with differences in mortality or other outcomes. Study design and methods: This was a single-center retrospective observational cohort study. We analyzed outcomes of patients who were intubated secondary to COVID-19 pneumonia between March 13, 2020, and December 12, 2020, at Henry Ford Hospital in Detroit, Michigan. Patients were categorized into two groups: early intubated (intubated within 24 hours of the onset of severe respiratory distress) and late intubated (intubated after 24 hours of the onset of severe respiratory distress). Demographics, comorbidities, respiratory rate oxygenation (ROX) index, sequential organ failure assessment (SOFA) score, and treatment received were compared between groups. The primary outcome was mortality. Secondary outcomes were ventilation time, intensive care unit stay, hospital length of stay, and discharge disposition. Post hoc and Kaplan-Meier survival analyses were performed. Results: A total of 110 patients were included: 55 early intubated and 55 late intubated. We did not observe a significant difference in overall mortality between the early intubated (43%) and the late intubated groups (53%) (p = 0.34). There was no statistically significant difference in patients\u27 baseline characteristics including SOFA scores (the early intubation group had a mean score of 7.5 compared to 6.7 in the late intubation group). Based on the ROX index, the early intubation group had significantly more patients with a reduced risk of intubation (45%) than the late group (27%) (p = 0.029). The early intubation group was treated with a high-flow nasal cannula at a significantly lower rate (47%) than the late intubation group (83%) (p \u3c 0.001). Significant differences in patient baseline characteristics, treatment received, and other outcomes were not observed. Post hoc analysis adjusting for SOFA score between 0 and 9 revealed significantly higher mortality in the late intubation group (49%) than in the early intubation group (26%) (p = 0.03). Patients in the 0 to 9 SOFA group who were intubated later had 2.7 times the odds of dying during hospital admission compared to patients who were intubated early (CI, 1.09-6.67). Interpretation: The timing of intubation for patients with severe COVID-19 pneumonia was not significantly associated with overall mortality or other patient outcomes. However, within the subgroup of patients with SOFA scores of 9 or lower at the time of intubation, patients intubated after 24 hours of the onset of respiratory distress had a higher risk of death than those who were intubated within 24 hours of respiratory distress. Thus, patients with COVID-19 pneumonia who are not at a high level of organ dysfunction may benefit from early mechanical ventilation

Impact of unseasonable flooding on women's food security and mental health in rural Sylhet, Bangladesh: a longitudinal observational study

Background Climate change will lead to more frequent and intensive flooding. In April, 2017, unseasonably early flooding led to the inundation of low-lying cropland before the rice harvest in northeastern Bangladesh. We describe coping strategies and quantify short-term and medium-term effects of flooding events on food security and depressive symptoms of women. Methods This observational study is part of the cluster-randomised Food and Agricultural Approaches to Reducing Malnutrition trial (FAARM; NCT02505711). Women self-reported flooding exposure on behalf of their households when surveyed (approximately 6 months after the event). Remote sensing analysis was used to detect the extent of the flooding. We collected data on household food security at baseline, depressive symptoms 4–5 months before the flooding, and coping strategies immediately after the event. We followed up on these outcome measurements for depressive symptoms and food security for up to 2·5 years after the flooding event. We used multilevel regression adjusting for intervention allocation and pre-flooding measures to quantify the flood's effect on household food security and women's mental health. Findings The FAARM trial included 2700 young women in 96 settlements in rural Sylhet, Bangladesh. 1335 (56%) of 2405 women reported that their household being greatly affected, with many losing a large part of their rice harvest. Borrowing money with interest was the most common coping strategy, with households paying back on average 1·5 times the borrowed amount. Greatly affected households had higher odds of food insecurity, with a decreasing effect with increasing time after the flood (odds ratio: 2·4 [p<0·0001] 0·5 years after; 1·6 [p<0·0001] 2·0 years after]; and 1·3 [p=0·012] 2·5 years after). Women in such households also had 1·45 times higher odds of depression (p=0·0001) 2·5 years after the flooding event. Interpretation The 2017 flooding event negatively affected food security and the mental health of women in rural Sylhet, Bangladesh, and few affected women received formal government support. To reduce the impact of future floods, livelihood adaptations and expansion of financial protection programmes are essential measures to pursue. Funding German Federal Ministry for Education and Research (Berlin, Germany) and UK Department for International Development (London, UK)

Lab-on-a-chip-based PCR-RFLP assay for the detection of Malayan box turtle (Cuora amboinensis)in the food chain and traditional Chinese medicines

The Malayan box turtle (Cuora amboinensis) (MBT) is a vulnerable and protected turtle species, but it is a lucrative item in the illegal wildlife trade because of its great appeal as an exotic food item and in traditional medicine. Although several polymerase chain reaction (PCR) assays to identify MBT by various routes have been documented, their applicability for forensic authentication remains inconclusive due to the long length of the amplicon targets, which are easily broken down by natural decomposition, environmental stresses or physiochemical treatments during food processing. To address this research gap, we developed, for the first time, a species-specific PCR-restriction fragment length polymorphism (RFLP) assay with a very short target length (120 bp) to detect MBT in the food chain; this authentication ensured better security and reliability through molecular fingerprints. The PCR-amplified product was digested with Bfa1 endonuclease, and distinctive restriction fingerprints (72, 43 and 5 bp) for MBT were found upon separation in a microfluidic chip-based automated electrophoresis system, which enhances the resolution of short oligos. The chances of any false negative identifications were eliminated through the use of a universal endogenous control for eukaryotes, and the limit of detection was 0.0001 ng DNA or 0.01% of the meat under admixed states. Finally, the optimized PCR-RFLP assay was validated for the screening of raw and processed commercial meatballs, burgers and frankfurters, which are very popular in most countries. The optimized PCR-RFLP assay was further used to screen MBT materials in 153 traditional Chinese medicines of 17 different brands and 62 of them were found MBT positive; wherein the ingredients were not declared in product labels. Overall, the novel assay demonstrated sufficient merit for use in any forensic and/or archaeological authentication of MBT, even under a state of decomposition

Response of Potato to Ash as an Alternative Source of Potassic Fertilizer

An experiment was carried out during rabi season of 2011-2012 in the experimental field of Soil Science, Bangladesh Agricultural University (BAU), Mymensingh. The objectives of present research work was to evaluate ash as an alternative source of potassic fertilizer for potato cultivation. The treatment combinations were i) Control (No K), ii) 100% K of recommended dose from muriate of potash (MoP), iii) 75% K from MoP+ 25% K from ash, iv) 50% K from MoP + 50% K from ash, v) 25% K from MoP + 75% K from ash, and vi) 100% K from ash. The result of the experiment indicated that various combinations of ash and MoP influenced the yield, yield contributing characters (length of the tubers, breadth of the tubers, number of tubers per hill, weight of tubers per hill, weight of ten tubers and gross yield of tubers per plot), K content of po tato as well as weed infestation. Among the treatments the highest yield was obtained from 50% K from MoP + 50% from ash (T3) treated plot. The K content in the potato tuber and weed infestation was also highest for that plot. Considering the yield contributing parameters, yield and number of weeds the T3 (50% K from MoP + 50% K from ash) treatment was found more suitable than others