The performance of field sampling for parasite detection in a wild passerine

Parasites can impact the behavior of animals and alter the interplay with ecological factors in their environment. Studying the effects that parasites have on animals thus requires accurate estimates of infections in individuals. However, quantifying parasites can be challenging due to several factors. Laboratory techniques, physiological fluctuations, methodological constraints, and environmental influences can introduce measurement errors, in particular when screening individuals in the wild. These issues are pervasive in ecological studies where it is common to sample study subjects only once. Such factors should be carefully considered when choosing a sampling strategy, yet presently there is little guidance covering the major sources of error. In this study, we estimate the reliability and sensitivity of different sampling practices at detecting two internal parasites-Serratospiculoides amaculata and Isospora sp.-in a model organism, the great tit Parus major. We combine field and captive sampling to assess whether individual parasite infection status and load can be estimated from single field samples, using different laboratory techniques-McMaster and mini-FLOTAC. We test whether they vary in their performance, and quantify how sample processing affects parasite detection rates. We found that single field samples had elevated rates of false negatives. By contrast, samples collected from captivity over 24 h were highly reliable (few false negatives) and accurate (repeatable in the intensity of infection). In terms of methods, we found that the McMaster technique provided more repeatable estimates than the mini-FLOTAC for S. amaculata eggs, and both techniques were largely equally suitable for Isospora oocysts. Our study shows that field samples are likely to be unreliable in accurately detecting the presence of parasites and, in particular, for estimating parasite loads in songbirds. We highlight important considerations for those designing host-parasite studies in captive or wild systems giving guidance that can help select suitable methods, minimize biases, and acknowledge possible limitations. Keywords: McMaster; fecal egg count; field sampling; mini‐FLOTAC; parasite infection; repeatability

The presence of air sac nematodes in passerines and near-passerines in southern Germany

Major climatic changes in conjunction with animal movement may be associated with the spread of parasites and their vectors into new populations, with potentially important consequences for population persistence. Parasites can evolve to adapt to unsuitable ecological conditions and take up refuge within new host species, with consequences for the population growth of the new host species. One parasite species that has likely been increasing its geographic range, and potentially infecting new hosts, is the recently described air sac nematode Serratospiculoides amaculata, in great tits (Parus major) in Slovakia. In this study, we screened wild birds for potential air sac nematode infection in a woodland area of southern Germany. We identified four additional host species: Eurasian nuthatch, great spotted woodpecker, greenfinch and robin. As infection by this group of nematodes can be highly pathogenic, we recommend further investigation into its potential risk to these populations

Does the availability of shade limit use of water troughs by desert birds?

Includes bibliographical referencesClimate change poses a major threat to living organisms, with maximum temperatures expected to continue to rise over the next few decades. Hot desert environments are particularly at risk because they experience high environmental temperatures, scarce vegetation, low productivity and unpredictable water sources. Endotherms such as birds face the challenge of maintaining a stable body temperature while avoiding dehydration. This study was carried out in the southern Kalahari, in South Africa's Northern Cape, where about 50% of bird species (36 species) depend on free-standing drinking water. Livestock farms within this area provide artificial water points, which benefit birds as well as livestock. This study determined the role of shade and cover in the use of these artificial water points by birds. An experiment was conducted at six waterholes using the Before-After, Control-Impact (BACI) design. After an initial baseline was established, three waterholes were shaded while the other three were left unshaded. Camera traps were used to record the pattern and intensity of water use by bird species at different times of the day and at varying air temperatures. A total of 36 bird species drank at the water holes, but data analysis was confined to the ten most abundant species. Of the ten, six species responded to the presence of shade/cover, with four species reacting positively (Cape Glossy Starling Lamprotornis nitens , Red - headed Finch Amadina erythrocephala , Black - throated Canary Serinus atrogularis , and Laughing Dove Spilopelia senegalensis ), four showing no significant change in drinking patterns, and two showing a decrease in visitor numbers when the site was shaded (Cape Turtle - Dove Streptopelia capicola, Namaqua Dove Oena capensis). This suggests that providing shade at waterholes is not a universal solution to the problem of increasing heat stress experienced by birds coming to drink. Certain species such as the Laughing Doves and Cape Turtle-Doves avoided waterholes during the warmest time of the day while the Namaqua Doves were frequent visitors at this time. However, the Laughing Dove took advantage of the shade provided at midday (warmest temperatures) as their numbers increased. The Red-headed Finch and Black-throated Canary also increased at water holes with temperature irrespective of the time of day. These patterns imply that the provision of shade modifies the behavior of some bird species in response to predation risk or heat stress. These species utilized shade at different times of day and with varying intensities as temperatures rose

The presence of artificial water points structures an arid-zone avian community over small spatial scales

Water plays a key role in avian thermoregulation, especially when environmental temperatures approach or exceed body temperature. Birds living in arid environments need to either be adapted to life with unreliable access to water, or engage in large-scale movements to access water. This study asked whether species richness and the distribution of bird species in the Kalahari landscape across scales of kilometres is predicted by the availability of open water sources. We conducted point count surveys for birds and modelled these as a function of habitat variables and distance to the nearest water source. We recorded 52 species during our point counts. Despite much variation in species richness scores at each point, species richness decreased with increasing distance from water. Granivorous birds were most dependent on water and showed higher probability of occurrence closer to water. Vegetation height impacted detectability for seven species, negatively for four and significantly positively for two. The provisioning of artificial water in the Kalahari is likely facilitating the presence and abundance of some bird species, notably seed eaters. Communities of birds will likely change if water points are closed or water becomes scarcer under climate change, which could well alter the ecosystem functioning of these regions.Keywords: air temperature, birds, climate change, distance, occupancy modelling, point count, surface water, wate

Blood Parasites of Semi-Domesticated and Wild Birds in Kaduna State, Nigeria

Wild birds interact with poultry with likelihood of exchange of blood parasites between the wild bird and poultry highlighting the need to understand wild bird parasites so as to reduce cross infection at the wild bird-poultry interface. There is paucity of data on blood parasites of wild birds in Kaduna State, Nigeria. This study investigates the prevalence and diversity of blood parasites among wild birds in Kaduna State. Blood of wild birds were examined from Febuary to June, 2012 for parasites by microscopic examination. Data were analyzed using Quantitative Parasitology software. Of 297 birds examined, 23.9 % had blood parasites with 39.4 % families and 39.3 % species infected. Parasites identified were Haemoproteus (7.7%), Plasmodium (16.2%), Leucocytozoon (2.7%), Aegyptionella (1.3%), and microfilariae (1.3%). There was a significant difference (p=0.0) between the prevalence of Aegyptionella, Leucocytozoon, Hemoproteus and Plasmodium. Some birds (4.1 %) had multiple blood parasite infection. Leucocytozoon was dectected in Columba livia, Streptopelia senegalensis, Meleagris gallopavo, Francolinus bicalcaratus, Hirundo aethopia and Pychonotus barbatus. Live poultry markets prevalence were Plasmodium (47.8 %), Haemoproteus (15.8 %) and Aegyptionella (2.6 %). Leucocytozoon prevalence was 4.2 % in free flying birds. Anchau had Plasmodium prevalence of 31.7 %. This study concludes that cross infection of blood parasites can occur at the wild bird-poultry interface and LWBMs encourages blood parasites transmission between countries. There is need for more studies on blood parasites of wild birds to understand their impact on the survival of wild bird species in Nigeria.Keywords: Blood parasites, Kaduna State, Live wild bird markets, Nigeria, Wild bird

The performance of field sampling for parasite detection in a wild passerine

Parasites can impact the behavior of animals and alter the interplay with ecological factors in their environment. Studying the effects that parasites have on animals thus requires accurate estimates of infections in individuals. However, quantifying parasites can be challenging due to several factors. Laboratory techniques, physiological fluctuations, methodological constraints, and environmental influences can introduce measurement errors, in particular when screening individuals in the wild. These issues are pervasive in ecological studies where it is common to sample study subjects only once. Such factors should be carefully considered when choosing a sampling strategy, yet presently there is little guidance covering the major sources of error. In this study, we estimate the reliability and sensitivity of different sampling practices at detecting two internal parasites—Serratospiculoides amaculata and Isospora sp.—in a model organism, the great tit Parus major. We combine field and captive sampling to assess whether individual parasite infection status and load can be estimated from single field samples, using different laboratory techniques—McMaster and mini-FLOTAC. We test whether they vary in their performance, and quantify how sample processing affects parasite detection rates. We found that single field samples had elevated rates of false negatives. By contrast, samples collected from captivity over 24 h were highly reliable (few false negatives) and accurate (repeatable in the intensity of infection). In terms of methods, we found that the McMaster technique provided more repeatable estimates than the mini-FLOTAC for S. amaculata eggs, and both techniques were largely equally suitable for Isospora oocysts. Our study shows that field samples are likely to be unreliable in accurately detecting the presence of parasites and, in particular, for estimating parasite loads in songbirds. We highlight important considerations for those designing host–parasite studies in captive or wild systems giving guidance that can help select suitable methods, minimize biases, and acknowledge possible limitations.publishe

The presence of air sac nematodes in passerines and near-passerines in southern Germany

Major climatic changes in conjunction with animal movement may be associated with the spread of parasites and their vectors into new populations, with potentially important consequences for population persistence. Parasites can evolve to adapt to unsuitable ecological conditions and take up refuge within new host species, with consequences for the population growth of the new host species. One parasite species that has likely been increasing its geographic range, and potentially infecting new hosts, is the recently described air sac nematode Serratospiculoides amaculata, in great tits (Parus major) in Slovakia. In this study, we screened wild birds for potential air sac nematode infection in a woodland area of southern Germany. We identified four additional host species: Eurasian nuthatch, great spotted woodpecker, greenfinch and robin. As infection by this group of nematodes can be highly pathogenic, we recommend further investigation into its potential risk to these populations