206 research outputs found
Response of combined abiotic stresses on maize (Zea mays L.) inbred lines and interaction among various stresses
In tropics, maize is largely grown as rain fed crop in marginal areas often faces drought and waterlogging simultaneouslyin same season that critically affects plant growth and development. The aim of the research was to studythe response of combined abiotic stresses and the interaction among various stresses on maize inbred plants. Inthe present study, eighty maize (Zea mays L.) inbred lines were screened, for multiple abiotic stresses at the vegetativestage. All the traits, observed were severely affected, in drought x low-N stress compare to waterloggingx low-N stress. However in both the stress combinations (drought x low-N and waterlogging x low-N) chlorophyllcontent decreases significantly, low-N stress has synergistic effect on the maize plants. Hence the overall effectof stress combination was negative causing enhanced damage to plants. Whereas, interaction of drought x waterloggingstress showed the antagonized each other response that primarily enhanced growth parameters (leafarea, plant height and stem diameter) and it has improved the tolerant mechanism of maize plants. Therefore weconcluded, the response of maize various plants exposed to combinations of stresses is depend on interactionof stresses
Purification and characterization of thermostable chitinase from a novel S. maltophilia strain
Aims: The presents study examines the purification and characterization of a chitinase from S. maltophilia SJ602 strainisolated from a soil sample collected from Jamia Hamdard, New Delhi.Methodology and Results: The purification steps included chitin affinity using colloidal chitin as the affinity matrix andcolumn chromatography using Sephadex G-100. The chitinase was purified to 66 fold having a yield of 17%. The molecular weight of the chitinase was found to be around 29 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The pH and temperature optima of the purified chitinase were found to be at pH 5.5 and60 °C, respectively. Conclusion, Significance and Impact of the study: Besides showing a significant yield, the enzyme has a highthermal stability which has its applicability in the recycling of chitin waste
Dengue virus capsid interacts with DDX3X-a potential mechanism for suppression of antiviral functions in dengue infection
Dengue virus is a pathogen of global concern and has a huge impact on public health
system in low- and middle-income countries. The capsid protein of dengue virus is
least conserved among related flavivirus and there is very limited information on the
role of cytosolic proteins that interact with dengue virus capsid. We identified DEAD
(Asp-Glu-Ala-Asp) Box Helicase 3, an X-Linked (DDX3X), cytosolic ATP-dependent RNA
helicase as a dengue virus capsid-interacting protein. We show that the N-terminal region
of capsid is important for interaction with DDX3X, while the N-terminal domain of DDX3X
seems to be involved in interaction with dengue capsid. DDX3X was down-regulated in
dengue virus infected cells at later stages of infection. Our results show that DDX3X is
an antiviral protein as suppression of DDX3X expression by siRNA led to an increase in
viral titers and overexpression of DDX3X led to inhibition of viral replication. Knock-down
of DDX3X did not affect induction of type I interferon response upon infection suggesting
that the effect of DDX3X knock-down is independent of the interferon-dependent
pathways that DDX3X modulates under normal conditions. Thus, our study identifies
DDX3X as a dengue virus capsid interacting protein and indicates a potential link between
the antiviral functions of DDX3X and dengue capsid at later stages of dengue infection
Perfil lipĂdico, compuestos volátiles y estabilidad oxidativa durante el almacenamiento del aceite de semilla de Opuntia ficus-indica marroquĂ
The fatty acids, sterol, tocopherol and volatile compositions of Moroccan cold-pressed cactus (Opuntia ficus-indica) seed oil were studied. The most abundant fatty acid, tocopherol and sterol were linoleic acid (60.6%), Îł-tocopherol (533 mg/kg) and β-sitosterol (6075 mg/kg), respectively. In this study, 23 volatile compounds were identified with perceivable odor attributes for 14 compounds. The oxidative quality of cactus seed oil was monitored over 4 weeks at 50 °C. Increases in PV, K232 and FFA were detected during the first two weeks as well as a decrease in the induction time; whereas no change was reported for the K270 values. The amount of total phenolic content increased until it reached 0.3 mg/kg and then decreased by the end of the storage period; while tocopherols started to decrease after the first week. The fat-free residue extracts showed a very strong effect to reduce the oxidation of linoleic acid. Consequently, the extracts were significantly more effective to bleach β-carotene in the β-carotene-linoleic acid assay in comparison with the control.Se estudiaron los ácidos grasos, esteroles, tocoferoles y la composiciĂłn volátil del aceite de semilla de cactus marroquĂ (Opuntia ficus-indica) prensado en frĂo. Los ácidos grasos, tocoferoles y esteroles más abundantes fueron el ácido linoleico (60,6%), Îł-tocoferol (533 mg/kg) y β-sitosterol (6075 mg/kg), respectivamente. En este estudio, se identificaron 23 compuestos volátiles con atributos perceptibles para 14 de ellos. La oxidaciĂłn del aceite de semilla de cactus fue monitoreada durante 4 semanas a 50°C. Se observĂł un aumento en el PV, K232 y FFA durante las dos primeras semanas y una disminuciĂłn en el tiempo de inducciĂłn, mientras que no se apreciaron cambios para los valores de K270. La cantidad de fenoles totales aumentĂł hasta alcanzar 0,3 mg/kg y luego disminuyĂł al final del almacenamiento, mientras que los tocoferoles comenzaron a disminuir despuĂ©s de la primera semana. Los extractos de residuos libres de grasa mostraron un efecto muy fuerte para reducir la oxidaciĂłn del ácido linoleico. En consecuencia, los extractos fueron significativamente más efectivos para blanquear el β-caroteno en el ensayo de β-caroteno-ácido linoleico en comparaciĂłn con el control
Compartmentalization of Cells Bearing "Rheumatic” Cell Surface Antigens in Peripheral Blood and Tonsils in Rheumatic Heart Disease
Monoclonal antibodies that recognize "rheumatic” antigens of peripheral blood non-T cells were used to study the compartmentalization of such cells in peripheral blood and tonsils of individuals with rheumatic heart disease (RHD) and suitable control subjects. The peripheral blood of most (71%) of the 42 individuals with RHD contained cells reacting with monoclonal antibody 83S19.23 or 256S.10, whereas these cells were present in only 17% of the 41 control subjects (P < .02). However, none of 21 individuals with RHD had such cells in their tonsils, although they were present in the tonsils of 50% of the 40 control subjects (P < .03). These results may reflect a failure in RHD of organ-specific homing of cells with the epitopes recognized by the antibodies. The presence of these cells in tonsils may be important in the immune response to streptococcal pharyngeal infection, and their absence in RHD may be involved in the unusual immune responses characteristic of this diseas
Host stage preference and parasitism behaviour of Aenasius bambawaleian an encyrtid parasitoid of Phenacoccus solenopsis
In Pakistan, the cotton mealybug, Phenacoccus solenopsis Tinsley (Sternorrhyncha (Homoptera): Pseudococcidae), is a serious pest of many cultivated plants. A parasitoid, Aenasius bambawalei Hayat (Hymenoptera: Encyrtidae), is associated with P. solenopsis. In order to mass rear A. bambawalei for a biological control program, it is important to investigate the parasitoid’s host stage preference and its parasitism behavior for P. solenopsis in order to optimize production. The present tudy showed that under both choice and no choice conditions, the parasitoid preferred 3rd instar and pre-reproductive host stage mealybugs for parasitism. Parasitoid larva developing inside the host exhibited a greater longevity, shorter developmental period and longer body size in these preferred host stages. Our study also confirmed that A. bambawalei showed no attraction to male mealybugs and no host feeding on any host stage was recorded. The ability of the parasitoid to effectively discriminate between suitable and non-suitable stages means that it is feasible to rear it on a mixed population
European Society of Cardiology quality indicators for the prevention and management of cancer therapy-related cardiovascular toxicity in cancer treatment.
AIMS: To develop quality indicators (QIs) for the evaluation of the prevention and management of cancer therapy-related cardiovascular toxicity. METHODS AND RESULTS: We followed the European Society of Cardiology (ESC) methodology for QI development which comprises (i) identifying the key domains of care for the prevention and management of cancer therapy-related cardiovascular toxicity in patients on cancer treatment, (ii) performing a systematic review of the literature to develop candidate QIs, and (iii) selecting of the final set of QIs using a modified Delphi process. Work was undertaken in parallel with the writing of the 2022 ESC Guidelines on Cardio-Oncology and in collaboration with the European Haematology Association, the European Society for Therapeutic Radiology and Oncology and the International Cardio-Oncology Society. In total, 5 main and 9 secondary QIs were selected across five domains of care: (i) Structural framework, (ii) Baseline cardiovascular risk assessment, (iii) Cancer therapy related cardiovascular toxicity, (iv) Predictors of outcomes, and (v) Monitoring of cardiovascular complications during cancer therapy. CONCLUSION: We present the ESC Cardio-Oncology QIs with their development process and provide an overview of the scientific rationale for their selection. These indicators are aimed at quantifying and improving the adherence to guideline-recommended clinical practice and improving patient outcomes
European Society of Cardiology quality indicators for the prevention and management of cancer therapy-related cardiovascular toxicity in cancer treatment.
AIMS: To develop quality indicators (QIs) for the evaluation of the prevention and management of cancer therapy-related cardiovascular toxicity. METHODS AND RESULTS: We followed the European Society of Cardiology (ESC) methodology for QI development which comprises (i) identifying the key domains of care for the prevention and management of cancer therapy-related cardiovascular toxicity in patients on cancer treatment, (ii) performing a systematic review of the literature to develop candidate QIs, and (iii) selecting of the final set of QIs using a modified Delphi process. Work was undertaken in parallel with the writing of the 2022 ESC Guidelines on Cardio-Oncology and in collaboration with the European Haematology Association, the European Society for Therapeutic Radiology and Oncology and the International Cardio-Oncology Society. In total, 5 main and 9 secondary QIs were selected across five domains of care: (i) Structural framework, (ii) Baseline cardiovascular risk assessment, (iii) Cancer therapy related cardiovascular toxicity, (iv) Predictors of outcomes, and (v) Monitoring of cardiovascular complications during cancer therapy. CONCLUSION: We present the ESC Cardio-Oncology QIs with their development process and provide an overview of the scientific rationale for their selection. These indicators are aimed at quantifying and improving the adherence to guideline-recommended clinical practice and improving patient outcomes
- …