Biochar: The Black Diamond for Soil Sustainability, Contamination Control and Agricultural Production

Production of biochars from agricultural wastes reduces significantly the volume and weight of the wastes, and hence, it can be considered as a promising means for managing the agricultural wastes. Biochar has received great interest during the last few years, due to its beneficial role to mitigate CO2 emission through enhancing the long-term carbon sequestration. The effects of biochar on soil properties vary widely, depending on the characteristics of soil and the biochar. Most types of biochars are of alkaline nature and of high C content. Addition of biochar to the soil can improve the cation exchange capacity enrich soil with the nutrients and enhance the microbial growth, and improve some soil physical properties such as water retention and aggregation. For contamination control, biochars have proven to be a suitable tool for controlling the contaminants in the environment. The high surface area, porous structure, alkaline nature, and the presence of functional groups characterized the biochar as alternative option for the remediation of heavy metal contaminated waters and soils. However, there is a lack of knowledge regarding the effects of biochar in the presence of mineral and/or organic fertilizers on the plant growth and nutrient transformation in soils. In addition, biochar is successfully used for treating the acid soils; therefore, future studies are needed to investigate the neutralization of alkaline performance of biochar to be used safely in alkaline soils

Synthesis, Characterization, and In Vivo Study of Some Novel 3,4,5-Trimethoxybenzylidene-hydrazinecarbothioamides and Thiadiazoles as Anti-Apoptotic Caspase-3 Inhibitors

The present study aims to discover novel derivatives as antiapoptotic agents and their protective effects against renal ischemia/reperfusion. Therefore, a series of new thiadiazole analogues 2a–g was designed and synthesized through cyclization of the corresponding opened hydrazinecarbothioamides 1a–g, followed by confirmation of the structure via spectroscopic tools (NMR, IR and mass spectra) and elemental analyses. The antiapoptotic activity showed alongside decreasing of tissue damage induced by I/R in the kidneys of rats using N-acetylcysteine (NAC) as an antiapoptotic reference. Most of the cyclized thiadiazoles are better antiapoptotic agents than their corresponding opened precursors. Particularly, compounds 2c and 2g were the most active antiapoptotic compounds with significant biomarkers. A preliminary mechanistic study was performed through caspase-3 inhibition. Compound 2c was selected along with its corresponding opened precursor 1c. An assay of cytochrome C revealed that there is an attenuation of cytochrome C level of about 5.5-fold, which was better than 1c with a level of 4.1-fold. In caspases-3, 8 and 9 assays, compound 2c showed more potency and selectivity toward caspase-3 and 9 compared with 1c. The renal histopathological investigation indicated normal renal tissue for most of the compounds, especially 2c and 2g, relative to the control. Finally, a molecular docking study was conducted at the caspase-3 active site to suggest possible binding modes

Synthesis, Characterization, and In Vivo Study of Some Novel 3,4,5-Trimethoxybenzylidene-hydrazinecarbothioamides and Thiadiazoles as Anti-Apoptotic Caspase-3 Inhibitors

The present study aims to discover novel derivatives as antiapoptotic agents and their protective effects against renal ischemia/reperfusion. Therefore, a series of new thiadiazole analogues 2a–g was designed and synthesized through cyclization of the corresponding opened hydrazinecarbothioamides 1a–g, followed by confirmation of the structure via spectroscopic tools (NMR, IR and mass spectra) and elemental analyses. The antiapoptotic activity showed alongside decreasing of tissue damage induced by I/R in the kidneys of rats using N-acetylcysteine (NAC) as an antiapoptotic reference. Most of the cyclized thiadiazoles are better antiapoptotic agents than their corresponding opened precursors. Particularly, compounds 2c and 2g were the most active antiapoptotic compounds with significant biomarkers. A preliminary mechanistic study was performed through caspase-3 inhibition. Compound 2c was selected along with its corresponding opened precursor 1c. An assay of cytochrome C revealed that there is an attenuation of cytochrome C level of about 5.5-fold, which was better than 1c with a level of 4.1-fold. In caspases-3, 8 and 9 assays, compound 2c showed more potency and selectivity toward caspase-3 and 9 compared with 1c. The renal histopathological investigation indicated normal renal tissue for most of the compounds, especially 2c and 2g, relative to the control. Finally, a molecular docking study was conducted at the caspase-3 active site to suggest possible binding modes

Synthesis, Characterization, and In Vivo Study of Some Novel 3,4,5-Trimethoxybenzylidene-hydrazinecarbothioamides and Thiadiazoles as Anti-Apoptotic Caspase-3 Inhibitors

The present study aims to discover novel derivatives as antiapoptotic agents and their protective effects against renal ischemia/reperfusion. Therefore, a series of new thiadiazole analogues 2a-g was designed and synthesized through cyclization of the corresponding opened hydrazinecarbothioamides 1a-g, followed by confirmation of the structure via spectroscopic tools (NMR, IR and mass spectra) and elemental analyses. The antiapoptotic activity showed alongside decreasing of tissue damage induced by I/R in the kidneys of rats using N-acetylcysteine (NAC) as an antiapoptotic reference. Most of the cyclized thiadiazoles are better antiapoptotic agents than their corresponding opened precursors. Particularly, compounds 2c and 2g were the most active antiapoptotic compounds with significant biomarkers. A preliminary mechanistic study was performed through caspase-3 inhibition. Compound 2c was selected along with its corresponding opened precursor 1c. An assay of cytochrome C revealed that there is an attenuation of cytochrome C level of about 5.5-fold, which was better than 1c with a level of 4.1-fold. In caspases-3, 8 and 9 assays, compound 2c showed more potency and selectivity toward caspase-3 and 9 compared with 1c. The renal histopathological investigation indicated normal renal tissue for most of the compounds, especially 2c and 2g, relative to the control. Finally, a molecular docking study was conducted at the caspase-3 active site to suggest possible binding modes.Peer reviewe

FUNGAL BIOTREATMENT OF OLIVE MILL WASTE WATER

The current study was aim to remediate olive mill waste water (OMWW) to reduce its phenol content and Chemical Oxygen Demand (COD) using fungal isolates. OMWW samples were drawn from the outlet of olive presser at Agriculture Research center, Egypt and characterized. Inoculating 25% diluted waste sample on Potato dextrose agar plates, incubated for 2 weeks at 25oC, resulted in 8 fungal isolates, of which isolate 5 was selected based on its capabilities to degrade phenol and reduce COD, compared to the rest of the obtained isolates. Comparison was conducted between the selected isolate and the fungus Pleurotus columbinus as a reference to test their potencies to degrade phenol and reduce COD in OMWW at concentrations from 100 to 10% over 4 weeks and results showed low degradability and weak tolerance of the two organisms at concentrations from 50 to 100%, while at 40, 30, 20 and 10%, phenol degradation and COD reduction over the 4 weeks treatment were more obvious. At all concentrations P. columbinus showed better competency for phenol degradation and COD reduction than isolate 5. Decolorization and growth of the two organisms were investigated in OMWW at 40 to 10% concentrations. P. columbinus, again, showed better competency over isolate 5 where it 79 and 49% of the color were removed after 4 weeks by P. columbinus and isolate 5, respectively. Total carbohydrate was also determined in the treated OMWW over 4 weeks and results showed it decreased from 6.05 to 5.2g/L in 40% OMWW and from 4.27 to 3.6 g/L in 30% OMWW, while it increased from 3.1 to 4.37g/L in 20% OMWW and from 1.46 to 3.9 g/L in 10% OMWW. Finally, the presence of indol acetic acid and gibberellins in 20 and 10% OMWW was tested as affect by the treatment with P. columbinus over the period of 4 weeks.Results showed that, after 4 weeks, IAA content decreased from 29.4 to 23.25 µg/ml in 20% OMWW, and slightly from 15.6 to 13,15 µg/ml in 10% OMWW. For gibberellins, the change after 4 weeks in 20% OMWW was not significant, that it decreased from 1.36 to 1.25mg, while it increased from 0.667 to 1.58 mg/ml in 10% waste in the same period. It can be concluded that remediating OMWW with P. columbinus can achieve a better results and the treated waste may be suitable for irrigation of crops

Universal gates for protected superconducting qubits using optimal control

We employ quantum optimal control theory to realize quantum gates for two protected superconducting circuits: the heavy-fluxonium qubit and the 0-$\pi$ qubit. Utilizing automatic differentiation facilitates the simultaneous inclusion of multiple optimization targets, allowing one to obtain high-fidelity gates with realistic pulse shapes. For both qubits, disjoint support of low-lying wave functions prevents direct population transfer between the computational-basis states. Instead, optimal control favors dynamics involving higher-lying levels, effectively lifting the protection for a fraction of the gate duration. For the 0-$\pi$ qubit, offset-charge dependence of matrix elements among higher levels poses an additional challenge for gate protocols. To mitigate this issue, we randomize the offset charge during the optimization process, steering the system towards pulse shapes insensitive to charge variations. Closed-system fidelities obtained are 99% or higher, and show slight reductions in open-system simulations.Comment: 12 pages, 6 figure

New Paracyclophanylthiazoles with Anti-Leukemia Activity: Design, Synthesis, Molecular Docking, and Mechanistic Studies

A new series of methyl 2-(2-(4′-[2.2]paracyclophanyl)-hydrazinylidene)-3-substituted-4-oxothiazolidin-5-ylidene)acetates 3a–f were synthesized from the reaction of paracyclophanyl-acylthiosemicarbazides 2a–f with dimethyl acetylenedicarboxylate. Based upon nuclear magnetic resonance (NMR), infrared (IR), and mass spectra (HRMS), the structure of the obtained products was elucidated. X-ray structure analysis was also used as unambiguous tool to elucidate the structure of the products. The target compounds 3a–f were screened against 60 cancer cell lines. They displayed anticancer activity against a leukemia subpanel, namely, RPMI-8226 and SR cell lines. The activity of compound 3a was found as the most cytotoxic potency against 60 cancer cell lines. Consequently, it was selected for further five doses analysis according to National Cancer Institute (NCI) protocol. The cytotoxic effect showed selectivity ratios ranging between 0.63 and 1.28 and between 0.58 and 5.89 at the GI50 and total growth inhibition (TGI) levels, respectively. Accordingly, compound 3a underwent further mechanistic study against the most sensitive leukemia RPMI-8226 and SR cell lines. It showed antiproliferation with IC50 = 1.61 ± 0.04 and 1.11 ± 0.03 µM against RPMI-8226 and SR cell lines, respectively. It also revealed a remarkable tubulin inhibitory activity, compared to colchicine with IC50 = 4.97 µM/mL. Caspase-3, BAX, and Bcl-2 assays for 3a using annexin V-FITC staining revealed significant pro-apoptotic activity. Furthermore, multidrug-resistant leukemia SR cells were used to show better resistance indices (1.285 ng/mL, 1.15-fold) than the reference. Docking studies with β-tubulin indicate that most of the tested compounds illustrated good binding at the colchicine binding site of the enzyme, especially for compound 3a, which made several interactions better than that of the reference colchicine