Feed Intake and Diet Selection by Sheep on Rangeland at Two Phenological Stages in North Kordofan State, Sudan

North Kordofan is a leading State of Sudan in animal and range resources. Animal production is traditional and extensive, depending on natural rangeland. Sheep rank first in importance amounting to about 12.9 million heads. This study was conducted at El Demokeya Forest Reserve, North Kordofan State, Sudan at two range sites, protected and open. The objectives were to determinate feed intake and assess the plant species selected by sheep and development of preference indices for use in managing the rangeland

Myometrial Abscess: A Complication of Myomectomy of a Large Lower-Uterine Segment Myoma

Background: This article describes a patient with a history of two early miscarriages. She presented with menorrhagia and a recurrence of multiple fibroids. Laparotomy and myomectomy were performed and were complicated by a myometrial abscess. The diagnosis and management of such a rare complication are described. Case: A 39-year-old African American female (gravida 3, para 1) presented with a history of two early miscarriages, menorrhagia, and a recurrence of multiple fibroids. An ultrasound (US) scan revealed an enlarged uterus with at least six uterine fibroids. Laparotomy and myomectomy were performed. Four weeks later, this patient presented with complaints of pelvic pain, nausea, vomiting, constipation, and a fever of 1 day's duration. A US scan revealed a slightly enlarged uterus caused by a possible infected hematoma. The patient was admitted to the hospital and she was started on intravenous antibiotics after a blood culture was obtained. A computed tomography (CT) scan of her abdomen and pelvis confirmed the diagnosis of a myometrial abscess. This patient underwent CT scan?guided placement of a ?pigtail? catheter in the myometrial abscess for continuous drainage. The result of the culture of the pus obtained from the abscess revealed the presence of multiple bacteria. The pigtail catheter was removed once there was no drainage 2 weeks after its initial insertion. Results: Two months after her discharge, a transvaginal US scan revealed that this patient's condition was essentially normal. Conclusions: This case report describes how a minimally invasive technique was used to manage a myometrial abscess, which is a complication of myomectomy. This report also illustrates the value of transvaginal US scanning for diagnosing such a pathology, and the roles US plays during management and follow-up. (J GYNECOL SURG 30:240)Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/140095/1/gyn.2013.0130.pd

Diet Selection by Goats on Rangeland of North Kordofan State, Sudan

This study was conducted at El Demokeya forest, North Kordofan State, Sudan. The objective was to evaluate goat diet botanical composition as an indicator for pasture quality. Bite counts were obtained from seven goats in protected and open rangeland sites. Percent plant cover in the two range sites were 81.9% and 87.5% respectively. Density was 260.9 and 181.9 plants / m2 respectively (P \u3c 0.05). At flowering, goat diet contained 10.8% ± 1.12 and 11.8% ± 5.94 CP in protected and open sites respectively. At seed set CP% was 8.1 ± 1.32 and 8.2 ± 3.01 respectively (P \u3e 0.01). Diet CP% was higher in diet than in forage biomass vegetation. At flowering, in protected range Acacia senegal (26.6%), Echinocloa colonum (11.34%) and Zaleya pentandra (9.08%) were the browse, grasses and forbs most selected, respectively. In the open range site Acacia senegal was 22.58%, Eragrostis tremula 13.78% and Zaleya pentandra 11.05% of the diet. At seed set, in the protected range site, the diet contained 18.36% Justica kotschyi, 15.02% Acacia senegal and 4.28% Eragrostis tremula.While in open range site Eragrostis tremula was 52.92%, Acacia senegal 25.58% and Chrozophora brocchiana 1.62%. Grasses and forbs with highest relative preference indeces (RPI) at flowering in protected range site were Cenchrus biflorus (RPI=2.05) and Justica kotschyi (RPI=7.93). In open range site the grass and forb with highest RPI were Echinocloa colonum (RPI=1.17) and Zornia glochidiata (RPI=6.7) respectively. Diet botanical composition is an indicator of plant preference and is useful in selecting plants for reseeding of deteriorated range and in identifying key species for range management

Cell cultures of the Manila clam and their possible use in biomonitoring and species preservation

A huge progress has been achieved in mammalian in vitro technique. Instead, of the many trials to develop marine invertebrate cell cultures, only a few have obtained them and only from few tissues. Since in vitro cell culture of invertebrates could be very useful for many aspects of basic and applied science, in this work we investigate and describe the development of a technique for the establishment of cell cultures from gill, mantle and gonadic tissue of the Manila clam (Ruditapes philippinarum). We maintained viable cultures for up to 25 days. Culture viability and proliferation were tested with 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) and with trypan blue, while an antibody against the ATP-dependent RNA helicase VASA, a protein expressed in the germline, and in multipotent stem cells of some animals, was used to verify the presence of these cell types. Following the described protocol: 1) explant resulted the better source to obtain cell cultures, when compared to enzymatic dissociation; 2) cultures of suspended cells were viable for longer period than adherent cells; 3) cell cultures obtained from tissues sampled in September-October performed better compared to other periods of the year, regarding maintenance and growth; 4) the tissue from which we obtained longer-lived cell cultures was gonadic tissue, especially form samples that show more undifferentiated germ cells and more VASA-stained cells. This study describes the challenges concerning the development of in vitro culture techniques for aquatic invertebrates

Single Level Lumbar Disk Herniation: Conventional Discectomy versus Interbody Fusion with Bilateral Pedicular Fixation

OBJECTIVE: The objective of the study was to compare between the conventional discectomy and the interbody fusion with bilateral pedicular screw fixation in the management of single level lumbar disk herniation. METHODS: This is a prospective study done on 50 patients (25 in each group) at Cairo University Hospitals in the period between October 2018 and June 2019 fulfilling the inclusion criteria. Diagnosis was made clinically with history (low back pain and sciatica),examination (motor power assessment, straight leg raising test), and radiological findings (MRI, CT and X-ray lumbar spine). The pain status (VAS) was pre and postoperatively evaluated and followed up every 3 months for 1 year. RESULTS: The study included 50 patients (25 in each group) (mean age 40.4 years old) with slight female predominance. The most common clinical findings presented at diagnosis were low back pain followed by lower limb pain in the form of claudication and sciatica. L5-S1 disk prolapse was the most common level affected in both groups followed by L4-5 level. Regarding the clinical outcome, there was statistical significance in the VAS of low back pain at 9 months and 1-year follow-up favoring the fusion group with mean VAS 0.40 and 1.32 (p < 0.05). CONCLUSION: Clinical outcome was excellent in both groups. However, the reduction in low back pain after surgery was greater in the fusion group (p < 0.05). The rate of recurrent disk herniation at the surgical level in the nonfusion group was higher, but intraoperative blood loss, operation time, length of hospital stay, and total cost of procedure were all less in the patients undergoing discectomy alone. Although there is still controversy regarding the pros and cons of fusion in association with disk excision without instability, fusion results were highly favorable

Prediction of moisture saturation levels for vinylester composite laminates : a data-driven approach for predicting the behavior of composite materials

Presented at the 34th International Conference of the Polymer Processing Society, May 24, 2018.This paper introduces a comprehensive, data-driven method to predict the properties of composite materials, such as thermo-mechanical properties, moisture saturation level, durability, or other such important behavior. The approach is based on applying data mining techniques to the collective knowledge in the materials field. In this article, first, a comprehensive database is compiled from published research articles. Second, the Random Forests algorithm is used to build a predictive model that explains the investigated material response based on a wide variety of material and process variables (of different data types). This advanced statistical learning approach has the potential to drastically enhance the design of composite materials by selecting appropriate constituents and process parameters in order to optimize the response for a specific application. This method is demonstrated by predicting the moisture saturation level for vinylester-based composite laminates. Using 90% of the available published data available as the training dataset, the Random Forests algorithm is used to develop a regression model for the moisture saturation level. Variables considered by the model include the manufacturing process, the fiber type and architecture, the fiber and void contents, the matrix filler type and content, as well as the conditioning environment and temperature. On this training data, the model proved to be a good fit with a prediction accuracy of R^2(training)=94.96%. When used to predict the moisture saturation level for the remaining unseen 10% of the compiled data, the model exhibited a prediction accuracy of R^2(test)=85.28%. Furthermore, the Random Forests model allows the assessment of the impact of the different variables on the moisture saturation level. The fiber type is found to be the most important determinant on the moisture saturation level in vinylester composite laminates.YesPeer reviewed for the proceedings of the 34t

Polymorphism of growth hormone gene and its association with wool traits in Egyptian sheep breeds

Growth hormone (GH) gene has been described as a candidate gene for marker-assisted selection in different farm animals. The present study was designed to identify the polymorphism in GH gene and its association with variation of wool traits in Egyptian sheep breeds. Wool and blood samples were collected from 42 animals including two breeds (Barki and Rahmani) and one crossbred (Rahmani x Awase). Measurements of wool traits were analyzed and involved staple strength (Str), staple length (STL), fiber diameter (FD) and clean fleece yield (CFW). DNA was extracted from blood samples and a 365-bp fragment from exon V was amplified by polymerase chain reaction (PCR). Single strand conformation polymorphisim (SSCP) analysis showed two conformational patterns. The pattern I was recorded to be more frequent (83.3, 92.86 and 90%) than pattern II (16.7, 7.14 and 10%) in Barki, Rahmani and crossbred, respectively. The sequence analysis showed one single nucleotide polymorphism (C/T). The pattern I (allele T) has been found to affect CFW and FD than pattern II (allele C). Whereas, C allele was more pronounced for Str and STL. These traits are the most important parameters determining commercial values of wool that are preferred for clothing or carpets industry. The nucleotide sequences of C and T alleles were submitted to GenBank and have the accession numbers: KT250511 and KT250512, respectively. In conclusion, the present results provide evidence that there is a single nucleotide polymorphism within GH gene in Egyptian sheep breeds. This mutation was found to have some effects on wool traits. Therefore our data show interesting prospects in future selection programs for improving wool industry.Key words: Sheep, wool, growth hormone (GH) gene, polymorphism, single strand conformation polymorphism (SSCP)

Yeast species-specific, differential inhibition of β-1,3-glucan synthesis by poacic acid and caspofungin

We sincerely thank Jeff Piotrowski and John Ralph for providing poacic acid, and David Perlin for providing C. glabrata fks1Δ and fks2Δ mutant strains and clinical isolates (DPL series) for this study. We thank Carol Munro, Sam Miller and Louise Walker for helpful discussions; and Raif Yuecel, Attila Bebes, and Linda Duncan in the Iain Fraser Cytometry Centre (IFCC) for FACS, and Kevin MacKenzie, Debbie Wilkinson, Gillian Milne, and Lucy Wright for microscopy at the University of Aberdeen core facilities. This work was supported by the Wellcome Trust (101873, 086827, 075470, & 200208) and MRC Centre for Medical Mycology (N006364/1), and Grants-in-Aid for Scientific Research from the Ministry of Education Culture, Sports, Science and Technology, Japan (24370002 and 15H04402 to Y.O.).Peer reviewedPublisher PD