Antiproliferative effects of isoprenoids from Sarcophyton glaucum on breast cancer MCF-7 cells

Purpose: To evaluate the anticancer activity of isoprenoids of Sarcophyton glaucum on MCF-7 cells and to investigate the potential synergistic effect of doxorubicin.Methods: Isolation and purification of isoprenoids were performed by applying different planar chromatographic methods (CC and PTLC). Further analyses of the isoprenoids by nuclear magnetic resonance (NMR) and mass spectrometry (MS) carried out to identify the compounds. Sulforhodamine- B (SRB) assay was used to determine the cytotoxic activity of the compounds against the MCF-7 human cell line. Flow cytometric analysis was used to assess their impact on cell cycle of  MCF-7. Combination index (CI), when the compounds were combined with  doxorubicin, was calculated to determine possible synergism. The isoprenoid  compounds were also incubated at ¼ or ½ of their respective half-maximal  concentration (IC50) with equimolar concentrations of doxorubicin.Results: Four known isoprenoid derivatives (1-4) were identified as 10(14)-aromadendrene (1), sarcophinediol (2), ent-deoxysarcophine (3) and sarcotrocheliol acetate (4). It was observed that cells accumulated in pre-G phase as well. CI of compound 3 with doxorubicin was 0.67 and 0.79, respectively, at ¼ and ½ of IC50, indicating overt synergism. This was confirmed by re-assessing the cell cycle stages of MCF-7 cells.Conclusion: The results indicate that compound 3 exhibits promising cytotoxicity as well as synergism with doxorubicin in MCF-7 cells. This is attributed, at least partly, to its ability to generate intercellular apoptosis induction.Keywords: Sarcophyton glaucum, Combination index, Antiproliferation, Isoprenoidal derivatives, 10(14)-Aromadendrene,Sarcophinediol, Deoxysarcophine,  Sarcotrocheliol acetate, Doxorubici

Secondary metabolites of the sponge-derived fungus Acremonium persicinum

This study reports the isolation and characterization of six new acremine metabolites, 5-chloroacremine A (4), 5-chloroacremine H (5), and acremines 0 (6), P (7), Q(8), and R (9), together with the known acremines A (1), F (2), and N (3) from the fungus Acremonium persicinum cultured from the marine sponge Anomoianthella rubra. The relative configuration of acremine F (2) was determined by analyses of proton coupling constant values and NOESY data, and the absolute configuration confirmed as (IS, 4S, 6R) by X-ray crystallographic analysis of the borate ester derivative 15. Acremines O, P, and R were each shown to be of 8R configuration by H-1 NMR analyses of MPA esters. The relative configurations suggested for acremines P and Q were each deduced by molecular modeling together with NOESY and coupling constant data. The (3)J(H-C) values in acremine P were measured using the pulse sequence EXSIDE, and the observed (3)J(H8-C4) of 5.4 Hz and small (3)J(H-C) values

Treatment of Alhagi Residues with Tartaric Acid for the Removal of Zn(II) Ions from Aqueous Solution

Alhagi cellulose tartrate (ACT) with a high carboxyl group content was prepared by treatment of Alhagi residues (AR) with tartaric acid (TA) at high temperature. Factors influencing the reaction were investigated, viz. tartaric acid concentration, dehydration temperature, particle size and dehydration time. The prepared Alhagi cellulose tartrate samples were characterized using FT-IR spectroscopy and by estimation of the carboxyl group content. The esterified samples were utilized for the adsorptive removal of Zn(II) ions from aqueous solution with all the factors influencing the process being studied. These factors were the pH value of the adsorbate solution, the adsorbent concentration, agitation time and temperature. The Langmuir and Freundlich models were applied to the data obtained from the adsorption studies. It was demonstrated that the Alhagi cellulose tartrate samples could be used effectively for the adsorption of Zn(II) ions from aqueous solutions. The thermodynamics constants of the adsorption process, viz. ΔH 0 , ΔS 0 and ΔG 0 , were evaluated. The results showed that the adsorption of Zn(II) ions onto ACT was exothermic and spontaneous. The adsorption data followed second-order kinetics, with the equilibrium data being only satisfactorily described by the Langmuir isotherm

Euryops arabicus Promotes Healing of Excised Wounds in Rat Skin: Emphasis on Its Collagen-Enhancing, Antioxidant, and Anti-Inflammatory Activities

Euryops arabicus Steud (E. arabicus) belongs to the family Asteraceae. It has several uses in folk medicine in the Arabian Peninsula. The current study aimed at evaluating the wound healing properties of the E. arabicus extract in rats. Primarily, E. arabicus successfully accelerated cell migration in vitro and it also showed no signs of dermal toxicity. Topical application of E. arabicus extract (5% or 20%) expedited healing of excised skin in rats. Histological examinations indicated that E. arabicus shortened epithelization period, stimulated fibroblast activity, and increased collagen deposition in wound tissues. The plant extract exerted antioxidant activity as evidenced by inhibition of GSH depletion and MDA accumulation and enhanced mRNA expression of Sod1 in wound tissues collected at the end of the experiment. Further, E. arabicus inhibited the rise of TNF-α and IL-1β in the skin wound region. The anti-inflammatory was confirmed by the observed down regulation of Ptgs2, Nos2, IL-6, and NF-κB mRNA expression. In addition, the extract enhanced the expression of TGF-β1 and HIF-1α in wounded skin tissues as indicated immunohistochemically. Conclusively, E. arabicus expedites excision wound healing in rats. Collagen-enhancing, anti-inflammatory, and antioxidant properties mediate the observed wound healing activity. These findings might contribute to our understanding of the ethnobotanical use of E. arabicus in wounds

Alnuheptanoid A: a new diarylheptanoid derivative from <i>Alnus japonica</i>

<div><p>Extensive chromatographic investigation of the ethanolic extract of <i>Alnus japonica</i> Steud stem bark led to the isolation of a new diarylheptanoid named alnuheptanoid A [(5<i>S</i>)-7-(3,4-dihydroxyphenyl)-1-(4-hydroxyphenyl)-5-methoxyheptan-3-one] (<b>8</b>), together with seven known diarylheptanoid derivatives: platyphyllenone (<b>5</b>), (5<i>S</i>)-1,7-bis(4-hydroxyphenyl)-5-methoxyheptan-3-one (<b>6</b>), 1-(3,4-dihydroxyphenyl)-7-(4-hydroxyphenyl)-4-hepten-3-one (<b>7</b>), hirsutenone (<b>9</b>), (5<i>R</i>)-<i>O</i>-methylhirsutanonol (<b>10</b>), hirsutanonol (<b>11</b>) and oregonin (<b>13</b>), three triterpenes: α-amyrin (<b>1</b>), betulinaldehyde (<b>3</b>) and betulinic acid (<b>4</b>), and two sterols: β-sitosterol (<b>2</b>) and daucosterol (<b>12</b>). Compound <b>6</b> was isolated for the first time from natural source. The structures of the isolated compounds were determined on the basis of spectroscopic measurements (UV, IR, HR-ESI-MS, 1D and 2D NMR).</p></div

In vitro and in vivo study of cucurbitacins-type triterpene glucoside from Citrullus colocynthis growing in Saudi Arabia against hepatocellular carcinoma

Chromatographic investigation of fruits obtained from Citrullus colocynthis, growing in Saudi Arabia, led to isolation of two compounds; Cucurbitacin E glucoside (Cu E, 1), and Cucurbitacin I glucoside (Cu I, 2). The chemical structures of 1 and 2, were elucidated by spectroscopic analyses include; 1D (H-1 and C-13) and 2D (COSY, HMQC and HMBC) NMR and ESI-MS spectroscopy. The in vitro cytotoxic activity against Hepatoma Cell Line (HepG2) and mice-bearing tumor of Ehrlich's ascites carcinoma (EAC) of the compounds were estimated. Both compounds had potent inhibitory activity on HepG2 with IC50 3.5 and 2.8 nmol/mL, respectively. In addition to these activities, the in vivo study employing EAC, showed the capability of both compounds to prolong the survival time, life span and normalize the biochemical parameters of the infected mice with EAC. (C) 2011 Elsevier B.V. All rights reserved