15 research outputs found
Micromonospora deserti sp. nov., isolated from the karakum desert
An isolate, 13K206(T), with typical morphological characteristics of the
genus Micromonospora was obtained during a study searching for novel
actinobacteria with biosynthetic potential from the Karakum Desert. A
polyphasic approach was adopted to determine taxonomic affiliation of
the strain. The strain showed chemotaxonomical properties consistent
with its classification in the genus Micromonospora such as meso- and
3-OH-A(2)pm in the cell-wall peptidoglycan, xylose in whole-cell
hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and
phosphatidylinositol as major polar lipids. The results of phylogenetic
analysis based on 16S rRNA gene sequences revealed that the strain was
closely related to `Micromonospora spongicola' S3-1(T), Micromonospora
nigra DSM 43818(T) and Micromonospora yasonensis DS3186(T) with sequence
similarities of 98.6, 98.5 and 98.4 \%, respectively. Digital DNA-DNA
hybridization and average nucleotide identity analyses in addition to
gyrB gene analysis confirmed the assignment of the strain to a novel
species within the genus Micromonospora for which the name
Micromonospora deserti sp. nov. is proposed. The type strain is
13K206(T) (=JCM 32583(T)=DSM 107532(T)). The DNA G+C content of the type
strain is 72.4 mol\%
Nonomuraea insulae sp nov., isolated from forest soil
WOS: 000447489900008PubMed ID: 29767331Strain H2R21(T), a novel actinobacterium, isolated from a forest soil sample collected from Heybeliada, Istanbul, Turkey, and a polyphasic approach was used for characterisation of the strain. Chemotaxonomic and morphological characterisation of strain H2R21(T) indicated that it belongs to the genus Nonomuraea. 16S rRNA gene sequence similarity showed that the strain is closely related to Nonomuraea purpurea 1SM4-01(T) (99.1%) and Nonomuraea solani CGMCC 4.7037(T) (98.4%). DNA-DNA relatedness values were found to be lower than 70% between the isolate and its phylogenetic neighbours N. purpurea 1SM4-01(T), N. solani CGMCC 4.7037(T) and Nonomuraea rhizophila YIM 67092(T). The whole cell hydrolysates of strain H2R21(T) were found to contain meso-diaminopimelic acid as the diagnostic diamino acid and glucose, madurose, mannose and ribose as the cell sugars. The polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, dihydroxy-phosphatidylethanolamine, phosphatidylinositol, glycophosphatidylinositol, two glycophospholipids and two unidentified lipids. The predominant menaquinones were identified as MK-9(H-4) and MK-9(H-6). The major fatty acids were found to be iso-C-16:0, iso-C-16:0 2OH and C-17:0 10-methyl. On the basis of DNA-DNA relatedness data and some phenotypic characteristics, it is evident that strain H2R21(T) can be distinguished from the closely related species in the genus Nonomuraea. Thus, it is concluded that strain H2R21(T) represents a novel species of the genus Nonomuraea, for which the name Nonomuraea insulae sp. nov. is proposed. The type strain is H2R21(T) (=DSM 102915(T) =CGMCC 4.7338(T) =KCTC 39769(T)).Ondokuz Mayis University (OMU)Ondokuz Mayis University [PYO.FEN.1904.13.004]This research was supported by Ondokuz Mayis University (OMU), project no. PYO.FEN.1904.13.004. We gratefully acknowledges Dr. Kannika Duangmal from Kasetsart University in Thailand for help to us in obtaining one of the type species
Polyphasic classification of Nonomuraea strains isolated from the Karakum Desert and description of Nonomuraea deserti sp. nov., Nonomuraea diastatica sp. nov., Nonomuraea longispora sp. nov. and Nonomuraea mesophila sp. nov.
Saygin, Hayrettin, Nouioui, Imen, Ay, Hilal, Guven, Kiymet, Cetin, Demet, Klenk, Hans-Peter, Goodfellow, Michael, Sahin, Nevzat (2020): Polyphasic classification of Nonomuraea strains isolated from the Karakum Desert and description of Nonomuraea deserti sp. nov., Nonomuraea diastatica sp. nov., Nonomuraea longispora sp. nov. and Nonomuraea mesophila sp. nov. International Journal of Systematic and Evolutionary Microbiology 70 (1): 636-647, DOI: 10.1099/ijsem.0.00380
Polyphasic classification of <em>Nonomuraea</em> strains isolated from the Karakum Desert and description of <em>Nonomuraea deserti</em> sp. nov., <em>Nonomuraea diastatica</em> sp. nov., <em>Nonomuraea longispora</em> sp. nov. and <em>Nonomuraea mesophila</em> sp. nov
Polyphasic classification of nonomuraea strains isolated from the karakum desert and description of nonomuraea deserti sp. Nov., Nonomuraea diastatica sp. nov., nonomuraea longispora sp. nov. and nonomuraea mesophila sp. nov
Five actinobacteria isolates, KC201(T), KC401, KC310(T), KC712(T) and
6K102(T), were recovered from the Karakum Desert during an investigation
of novel actinobacteria with biotechnological potential. A polyphasic
approach confirmed the affiliation of the strains to the genus
Nonomuraea. The strains showed chemotaxonomic and morphological
properties consistent with their classification in the genus Nonomuraea.
Furthermore, these strains clearly distinguished and formed well
supperted clades in phylogenetic and phylogenomic trees. Low ANI and
dDDH values and distinguishing phenotypic properties between isolates
KC201(T), KC310(T), KC712(T) and 6K102(T) showed that these strains
belonged to novel Nonomuraea species, the names proposed for these taxa
are Nonomuraea deserti sp. nov., Nonomuraea diastatica sp. nov.,
Nonomuraea longispora sp. nov. and Nonomuraea mesophila sp. nov., with
the type strains KC310(T) (=CGMCC 4.7331(T) =DSM 102919(T) =KCTC
39774(T)), KC712(T) (=CGMCC 4.7334(T) =DSM 102925(T) =KCTC 39776(T)),
KC201(T) (=CGMCC 4.7339(T) =DSM 102917(T) =KCTC 39781(T)) and 6K102(T)
(=CGMCC 4.7541(T) =JCM 32916(T)), respectively
A constrained Nash bargaining approach to the stock rationing problem in multi-echelon supply chains
On the Origin of Phosphorylated Biomolecules
Phosphorus is a key element in biology, serving in cellular replication, metabolism, and structure. The versatility of phosphorus in biology is due to several unique chemical characteristics that rely on its electronic structure and geochemical abundance. The formation of phosphorylated biomolecules and their activated precursors have hence been a major focus of prebiotic syntheses for the past 50 years. This chapter highlights the basic chemical and physical features that make phosphorus chemicals so valuable within contemporary biochemistry, the putative prebiotic routes to phosphorylated biomolecules, and a growing role for reduced oxidation state phosphorus compounds, including those derived from meteorites, in the development of life on the Earth. We distinguish three primary forms of biological phosphates that form an energetic hierarchy: (i) stable phosphorylated biomolecules that are unreactive and in which the P provides a structural or binding handle; (ii) energetic condensed phosphates including ATP which store metabolic energy; and (iii) reactive phosphorylated biomolecules which are generated during metabolism and transfer phosphates and energy to condensed phosphates for energy storage. We suggest here that: (1) precursors to modern biologic phosphates likely included reduced oxidation state phosphorus compounds; (2) ATP as the main metabolic energy transfer agent likely arose well after the origin of life, and was likely co-opted from its role as a RNA building block into its metabolic role