Annexin A2 antibodies but not inhibitors of the annexin A2 heterotetramer impair productive HIV-1 infection of macrophages in vitro

During sexual transmission of human immunodeficiency virus (HIV), macrophages are initial targets for HIV infection. Secretory leukocyte protease inhibitor (SLPI) has been shown to protect against HIV infection of macrophages through interactions with annexin A2 (A2), which is found on the macrophage cell surface as a heterotetramer (A2t) consisting of A2 and S100A10. Therefore, we investigated potential protein-protein interactions between A2 and HIV-1 gp120 through a series of co-immunoprecipitation assays and a single molecule pulldown (SiMPull) technique. Additionally, inhibitors of A2t (A2ti) that target the interaction between A2 and S100A10 were tested for their ability to impair productive HIV-1 infection of macrophages. Our data suggest that interactions between HIV-1 gp120 and A2 exist, though this interaction may be indirect. Furthermore, an anti-A2 antibody impaired HIV-1 particle production in macrophages in vitro, whereas A2ti did not indicating that annexin A2 may promote HIV-1 infection of macrophages in its monomeric rather than tetrameric form

Reciprocal Prospective Relationships Between Loneliness and Weight Status in Late Childhood and Early Adolescence

Adolescents who do not conform to weight ideals are vulnerable to disapproval and victimization from peers in school. But, missing from the literature is a prospective examination of weight status and feelings of loneliness that might come from those experiences. Using data from the Québec Longitudinal Study of Child Development, we filled that gap by examining the prospective associations between loneliness and weight status when the sample was aged 10 to 13 years. At ages 10, 12, and 13 years, 1042 youth (572 females; 92% from French speaking homes) reported on their loneliness and were weighed and measured. Family income sufficiency was included in our analyses given its relationship with weight status, but also its possible link with loneliness during early adolescence. The findings showed that (1) weight status and loneliness were not associated concurrently; (2) weight status predicted increases in loneliness from ages 12 to 13 years; and (3) loneliness predicted increases in weight from ages 12 to 13 years among female adolescents, but weight loss among male adolescents. The fact that loneliness was involved in weight gain for females suggests that interventions focused on reducing loneliness and increasing connection with peers during early adolescence could help in reducing obesity

Shyness, Unsociability, and Socio-Emotional Functioning at Preschool: The Protective Role of Peer Acceptance

In present study, we examined the protective role of peer acceptance in the links between two subtypes of social withdrawal (shyness, unsociability) and indices of young children’s socio-emotional functioning. Participants were N = 112 Italian preschool children (n = 54 boys) aged 36–74 months (M = 56.85 months, SD = 10.14). Multi-source assessments included: (1) parental ratings of children’s shyness and unsociability; (2) teacher ratings of children’s internalizing problems, externalizing problems, and social competence; (3) child interview assessments of preference for solitary play; and (4) peer (sociometric) ratings of peer acceptance. Among the results, shyness was associated with internalizing problems at preschool, whereas unsociability was related to a preference for solitary play. In addition, results from multiple regression analyses indicated significant interactions between peer acceptance and both shyness and unsociability in the association with indices of socio-emotional functioning. For example, at lower levels of peer acceptance, shyness was positively related to children’s preference for solitary play, whereas children’s unsociability was associated with externalizing problems. In contrast, these relations were attenuated at higher levels of peer acceptance. Findings are discussed in term of the potential protective role of young children’s peer acceptance for different subtypes of social withdrawal during early childhood