Determination of microbiological quality and histamine levels in rainbow trouts (Oncorhynchus mykiss)

NO ABSTRACT AVAILABLEThis study aims to determine the microbiological quality and histamine levels of fresh-marketed rainbow trouts (Oncorhynchus mykiss). The total mesophilic aerobic bacteria (TMAB), total psychrophilic aerobic bacteria (TPAB), coliform, fecal coliform, Enterobacteriaceae, Pseudomonas, Staphylococcus-Micrococcus and mold-yeast numbers were detected as 4.24, 4.00, 1.10, 0.17, 1.50, 3.05, 0.28 and 3.82 log10 cfu/g, respectively. Yersinia enterocolitica, Listeria monocytogenes, Escherichia coli O157:H7 and Vibrio spp bacteria were found in 18 (37.5%), 14 (29.1%), 8 (16.6%) and 14 (29.1%) respectively. Twenty-eight out of 51 Listeria isolates (54.9%) were verified through PCR analysis as L. monocytogenes. It was determined that the histamine level in the samples was above the detectable level (>2.5 mg/kg) in 8 of the samples (16.6%)determined using an ELISA method. It was concluded that the detection of foodborne pathogens in fresh-marketed fish samples potentially constitutes a public health hazard

JURIDICAL ANALYSIS OF E-CONSENT SERVICES BASED ON LAW NUMBER 17 OF 2023 ON HEALTH AT ZAINAL ABIDIN HOSPITAL, PAGAR ALAM, WAY KANAN

The“development of information technology in the health sector has significantly changed how health services are delivered and regulated. One important change is the shift from conventional informed consent (oral or written) to e-consent or electronic technology-based consent. According to Article 335 of Law No. 17 Year 2023 on Health. Informed consent is a key element in ethical and legal medical practice. The main principle is the individual must give that consent. Based on Article 2 paragraph (1) Permenkes RI Number 290 /Menkes / Per / III / 2008 concerning Medical Action Consent states that “All actions that will be carried out on patients must obtain consentâ€, therefore the article aims to analyze the rules for implementing e-consent and the obstacles to the application of e-consent in health services, especially at the Hospital Rsud Zainal Abidin Pagar Alam Way Kanan. This research method uses normative Judicial legal research. The results showed that the rules for e-consent have been regulated in Indonesian legislation, but the use of e-consent has weaknesses, such as patients, doctors, and other medical personnel not being accustomed to changes from conventional to digitalization. In addition, inadequate equipment from the Hospital is also an obstacle to implementing this E-consent service.â€The proposed suggestion is the need for improvement and training regarding the rules and work practice training related to e-consent between doctors, medical personnel, and patients at Zainal Pagar Alam Way Kanan Hospital

Measurement of functional microcirculatory geometry and velocity distributions using automated image analysis

This study describes a new method for analyzing microcirculatory videos. It introduces algorithms for quantitative assessment of vessel length, diameter, the functional microcirculatory density distribution and red blood-cell (RBC) velocity in individual vessels as well as its distribution. The technique was validated and compared to commercial software. The method was applied to the sublingual microcirculation in a healthy volunteer and in a patient during cardiac surgery. Analysis time was reduced from hours to minutes compared to previous methods requiring manual vessel identification. Vessel diameter was detected with high accuracy (>80%, d > 3 pixels). Capillary length was estimated within 5 pixels accuracy. Velocity estimation was very accurate (>95%) in the range [2.5, 1,000] pixels/s. RBC velocity was reduced by 70% during the first 10 s of cardiac luxation. The present method has been shown to be fast and accurate and provides increased insight into the functional properties of the microcirculation

Cytotoxic activities of new iron(III) and nickel(II) chelates of some S-methyl-thiosemicarbazones on K562 and ECV304 cells

The S-methyl-thiosemicarbazones of the 2- hydroxy-R-benzaldehyde (R= H, 3-OH 3-OCH3 or 4-OCH3) reacted with the corresponding aldehydes in the presence of FeCl3 and NiCl2. New ONNO chelates of iron(III) and nickel (II) with hydroxy- or methoxy-substitued N1,N4-diarylidene-Smethyl- thiosemicarbazones were characterized by means of elemental analysis, conductivity and magnetic measurements, UV-Vis, IR and 1H-NMR spectroscopies. Cytotoxic activities of the compounds were determined using K562 chronic myeloid leukemia and ECV304 human endothelial cell lines by MTT assay. It was determined that monochloro N1-4- methoxysalicylidene-N4-4-methoxysalicylidene-S-methylthiosemicarbazidato- iron(III) complex showed selective anti-leukemic effects in K562 cells while has no effect in ECV304 cells in the 0.53 μg/ml (IC50) concentrations. Also, some methoxy-substitued nickel(II) chelates exhibit high cytotoxic activitiy against both of these cell lines in low concentrations. Cytotoxicity data were evaluated depending on cell lines origin and position of the substituents on aromatic rings