Procjena odnosa srednjeg volumena trombocita, širine distribucije eritrocita i indeksa volumena trombocita s težinom bolesti kod bolesnika s COVID-19

Coronavirus was first detected in three severe pneumonia cases in Wuhan, China, in December 2019. Studies on red cell distribution width (RDW-CV) and mean platelet volume (MPV) laboratory parameters, which can be examined in complete blood count in COVID-19 patients, are still very limited. However, to the best of our knowledge, there are no studies examining the relationship between platelet volume index (PVI) and disease severity in COVID-19 patients, which was evaluated in this study. The aim of this study was to evaluate the relationship of disease severity in COVID-19 patients with their MPV, RDW, and PVI parameters. The study included 92 COVID-19 patients as a study group and 84 healthy individuals as control group. All laboratory data and radiological images were scanned retrospectively from patient files and hospital information system. Evaluation of the RDW-CV and MPV blood parameters, and PVI measured in COVID-19 patients yielded statistically significant differences according to the disease severity. We suggest that RDW-CV and PVI, evaluated within the scope of the study, may be the parameters that should be considered in the early diagnosis of the disease, from the initial stages of COVID-19. In addition, we think that the RDW-CV and MPV laboratory parameters, as well as PVI, which all are simple, inexpensive and widely used hematologic tests, can be used as important biomarkers in determining COVID-19 severity and mortality.Koronavirus je prvi put otkriven kod tri slučaja teške upale pluća u Wuhanu, Kina u prosincu 2019. godine. Istraživanja laboratorijskih parametara širine distribucije eritrocita (RDW-CV) i srednjeg volumena trombocita (MPV) koji se mogu testirati unutar kompletne krvne slike kod bolesnika s COVID-19 još uvijek su vrlo ograničena. Međutim, prema našim saznanjima, nema istraživanja koja bi se bavila odnosom indeksa volumena trombocita (PVI) i težine bolesti kod ovih bolesnika, a upravo to smo ispitivali u našem istraživanju. Cilj studije bio je procijeniti odnos težine bolesti u bolesnika s COVID- 19 s parametrima MPV, RDW i PVI. Istraživanje je obuhvatilo 92 bolesnika s COVID-19 i 84 zdrave osobe kao kontrolna skupina. Svi laboratorijski podaci i radiološke snimke preslikane su retrospektivno iz bolesničkih kartona i bolničkog informacijskog sustava. Procjena krvnih parametara RDW-CV i MPV, kao i PVI izmjerenih u bolesnika s COVID-19 pokazala je statistički značajne razlike prema težini bolesti. Predlažemo da bi RDW-CV i PVI koji su istraživani u ovoj studiji mogli poslužiti kao parametri u ranoj dijagnostici bolesti već u početnom stadiju COVID-19. Uz to, smatramo da bi se laboratorijski parametri RDW-CV i MPV te PVI kao jednostavne, jeftine i široko primjenjivane hematološke pretrage mogli primijeniti kao važni biološki biljezi u utvrđivanju težine i smrtnosti COVID-19

Evaluation of the relation of Platelet Volume Index, MPV and RDW values with mortality in spontaneous intraparenchymal hemorrhages

Introduction: Intracerebral Spontaneous Hemorrhage (ISH) is a sudden hemorrhage into the brain parenchyma as a result of a rupture of the cerebral vessels that are not related to traumas. The purpose of the present study was to evaluate the relation of mortality with Platelet Volume Index (PVI) scoring systems, which have been reported in a small number of studies in terms of blood RDW, MPV, platelet and intracranial hemorrhages in patients diagnosed with intraparenchymal hemorrhage in Emergency Departments. Methods: This study was performed retrospectively by examining patient files of patients who came to Emergency Medicine Clinic between 01.01.2019-31.12.2019. As a control group for comparison of blood parameters, blood parameters of 72 patients suitable for the same age population, without intraparenchymal haemorrhage.Results: A total of 54.10% (n=85) intraparenchymal bleeding patients (IPC) and 45.90% (n=72) healthy control groups (HCG) were included in the study. There was no statistically significant difference between the clinical results of IPC groups in terms of mortality of RDW parameter and MPV parameter (p=0.930; p=0.118). When PVI ratio was evaluated in IPC group and HCG; the mean PVI (MPV/Platelet ratio) in the IPC group was 4.37±1.66, and the PVI (MPV/Platelet ratio) in the HCG was 3.89±1.02.A statistically significant difference was found between the PVI in the patient group and the HCG.A statistically significant difference was found between the PVI ratio clinical results of the patients with IPC (p=0.043).Conclusion: Spontaneous Intraparenchymal hemorrhage are among the leading causes of stroke-induced mortality and disability. The Hemphill Score and hemorrhage volume are important factors in mortality evaluation in intraparenchymal hemorrhage. There are not many studied conducted on Platelet Volume Index, and it is an important marker in predicting mortality, especially in these patients

Antibody-Conjugated Electrospun Nanofibers for Electrochemical Detection of Methamphetamine

Multifunctional electrospunnanofibers (ENs) with improved propertieshave increased attention nowadays. Their insoluble forms in waterwith decreased hydrophobicity are desired for the immobilization ofbiological molecules. Also, the addition of functional groups on thebackbone provides the conjugation of biomolecules onto the surfaceof ENs via covalent bonds to increase their stability. Here, poly-(vinylidenefluoride) (PVDF) was chosen to prepare a platform, which is insolublein water, and polyethylenimine (PEI) was used to add amine groupson the surface of ENs to bind biological molecules via covalent conjugation.So, PVDF-PEI nanofibers were prepared on a glassy carbon electrodeto immobilize an antimethamphetamine antibody (Anti-METH) as a modelbiomolecule. The obtained PVDF-PEI/Anti-METH was used for the bioelectrochemicaldetection of methamphetamine (METH), a common illicit drug. Bioelectrochemicaldetection of METH on PVDF-PEI/Anti-METH-coated electrodes was carriedout by voltammetry in the range of 2.0-50 ng/mL METH. Moreover,the effect of dansyl chloride (DNC) derivatization of METH on thesensitivity of PVDF-PEI/Anti-METH was tested. Finally, METH analysiswas carried out in synthetic body fluids. The obtained results showedthat PVDF-PEI ENs can be adopted as an immobilization matrix for thebiorecognition elements of biobased detection systems, and the derivativeof METH (METH-DNC) increased the sensitivity of PVDF-PEI/Anti-METH.Authors thank Ege University Research Funds (Project number: FYL-2018-20015). Part of the work was funded by the Republic of Turkey, Ministry of Development (Project Grant No: 2016 K121190). G.A. thanks The Scientific and Technological Research Council of Turkiye (TUBITAK) 2210/D National Industrial MSc/MA Scholarship Program. The authors acknowledge the Ege University Application and Research Center for Testing and Analysis (EGE-MATAL) and the Izmir Katip Celebi University Central Research Laboratory Application and Research Center for XPS and SEM analysis, respectively.Ege University Research Funds [FYL-2018-20015]; Republic of Turkey, Ministry of Development [2016 K121190]; Scientific and Technological Research Council of Turkiye(TUBITAK) 2210/D National Industrial MSc/MA Scholarship Progra

Comparison of first admission hemogram parameters and chest computed tomography findings of pediatric COVID-19 patients

Aim : We use computed tomography, which is one of the frequently used imaging tests, both as a disease diagnosis method and to follow the clinical course in COVID-19 patients. This also means radiation exposure. Radiation exposure, especially in pediatric patients, can cause life-threatening diseases. Is there a blood parameter that will reduce this undesirable event and allow estimation of computed tomography findings? Are hemogram analysis, one of the most commonly used blood tests, and tomography findings of the disease related? We designed this study based on the questions. Material and Methods: Among the patients under the age of 18 who applied to the emergency department, those with a positive reverse transcription-polymerase chain reaction (RT-PCR) and chest CT and hemogram were included in the study. Chest CT findings were classified according to the CO-RADS classification. We compared the CO-RADS classification with hemogram parameters and the ratios of these parameters. Results: Platelet-to-lymphocyte ratio (PLR) rates were found to be significantly lower as imaging findings became more severe (<0.05). The ratio of MedianPlatelet Volume and Platelet (MPV/Plt) was found to be significantly higher as the imaging findings worsened (p<0.05). When the relationship between laboratory parameters according to imaging groups in our study was evaluated, there was a moderate negative correlation between lymphocyte and platelet levels and imaging findings (p<0.05). A moderate positive correlation with the monocyte level was found (p<0.05). Discussion: For the CO-RADS classification, it can be said that the patient was established to classify possible COVID-19 patients only according to chest CT. There is no study in the literature on the classification of pediatric patients with RT-PCR positive definite COVID-19 according to chest CT scans and the comparison of laboratory findings of patients with this classification. The combination of laboratory parameters and CO-RADS classification will guide clinicians in pediatric COVID-19 patient management

Evaluation of the relation of Platelet Volume Index, MPV and RDW values with mortality in spontaneous intraparenchymal hemorrhages

Introduction: Intracerebral Spontaneous Hemorrhage (ISH) is a sudden hemorrhage into the brain parenchyma as a result of a rupture of the cerebral vessels that are not related to traumas. The purpose of the present study was to evaluate the relation of mortality with Platelet Volume Index (PVI) scoring systems, which have been reported in a small number of studies in terms of blood RDW, MPV, platelet and intracranial hemorrhages in patients diagnosed with intraparenchymal hemorrhage in Emergency Departments. Methods: This study was performed retrospectively by examining patient files of patients who came to Emergency Medicine Clinic between 01.01.2019-31.12.2019. As a control group for comparison of blood parameters, blood parameters of 72 patients suitable for the same age population, without intraparenchymal haemorrhage.Results: A total of 54.10% (n=85) intraparenchymal bleeding patients (IPC) and 45.90% (n=72) healthy control groups (HCG) were included in the study. There was no statistically significant difference between the clinical results of IPC groups in terms of mortality of RDW parameter and MPV parameter (p=0.930; p=0.118). When PVI ratio was evaluated in IPC group and HCG; the mean PVI (MPV/Platelet ratio) in the IPC group was 4.37±1.66, and the PVI (MPV/Platelet ratio) in the HCG was 3.89±1.02.A statistically significant difference was found between the PVI in the patient group and the HCG.A statistically significant difference was found between the PVI ratio clinical results of the patients with IPC (p=0.043).Conclusion: Spontaneous Intraparenchymal hemorrhage are among the leading causes of stroke-induced mortality and disability. The Hemphill Score and hemorrhage volume are important factors in mortality evaluation in intraparenchymal hemorrhage. There are not many studied conducted on Platelet Volume Index, and it is an important marker in predicting mortality, especially in these patients