IDENTIFIKASI STOK IKAN KAKAP PUTIH (Lates calcarifer Bloch, 1790) MENGGUNAKAN KARAKTER MORFOMETRIK

Penelitian ini bertujuan untuk menganalisis diferensiasi morfologi yang terjadi di antara stok ikan kakap putih, barramundi (Lates calcarifer Bloch, 1790) di perairan pantai Kabupaten Bone, Wajo, Takalar, dan Kalimantan Utara. Diferensiasi karakter morfometrik dianalisis menggunakan fungsi dikskriminan. Hasil penelitian menunjukkan bahwa ikan kakap putih dari perairan Teluk Bone, Selat Makassar dan pesisir Pantai Sekatak Kalimantan Utara bersifat monophyletic. Aliran gen dan migrasi di antara populasi atau stok ikan kakap putih sangat terbatas atau hampir tidak ada. Stok ikan kakap putih di pesisir pantai Desa Akkotengeng Kabupaten Wajo dan stok di pesisir pantai dan daerah aliran sungai (DAS) Sungai Saro Kabupaten Takalar melakukan rekruitmen sendiri. Terdapat satu individu ikan kakap putih di perairan pantai Siwa Kabupaten Wajo dan Cenrana Kabupaten Bone (kedua stok berlokasi di Teluk Bone) yang memiliki fenotipe (morfologi) yang mirip dengan ikan kakap putih di perairan pantai Bulungan Kalimantan Utara. Analisis kontribusi 15 karakter morfometrik terhadap fungsi kanonik menunjukkan bahwa karakter yang menjadi penciri di antara stok adalah tinggi badan, caudal peduncle, dan diameter mata. Hasil discriminant function analysis (DFA) menunjukkan bahwa terdapat tiga populasi lokal yang signifikan berbeda secara fenotipe, sehingga dalam pengelolaannya dibutuhkan manajemen yang berbeda.Kata kunci: Asian seabass, barramundi, karakter morfometrik, Lates calcarife

Analysis of Expressed Sequence Tags (EST) Obtained from Common carp, Cyprinus carpio L., Head Kidney Cells After Stimulation by CpG oligodeoxynucleotides

We analyzed genes expressed from head kidney of common carp Cyprinus carpio L. treated with CpG oligodeoxynucleotides. The results of single-pass sequencing of expressed sequence tags (ESTs) from 88 clones (AU312478-AU312561) from kidney cDNA are presented. Out of 88 clones 84 (95.5 %) matched with nucleic acid and/or amino acid sequences, whereas the remaining 4 (4.5 %) clones did not show any significant homology to the sequences in the databases. Immune related cDNA clones identified from kidney were granulin2, CCAAT/enhancer binding protein, immunoglobulin heavy chain variable region, lectin, lysozyme C, interleukin-4 receptor alpha chain, cathepsin L preproprotein, CD9 protein and Granulin 1 were identified

Genetic patterns of the corals Euphyllia glabrescens and Lobophyllia corymbosa across the Indonesian Archipelago

Abstract. Jompa J, Umar W, Yusuf S, Tassakka ACM, Limmon GV, Rahmi, Putri AP, Halwi, Tamti H, Moore AM. 2020. Genetic patterns of the corals Euphyllia glabrescens and Lobophyllia corymbosa across the Indonesian Archipelago. Biodiversitas 21: xxxx. Scleractinian corals can reproduce in several ways, with two main sexual reproduction modes known as brooding and broadcast spawning. In this study, we described patterns of genetic variation within and connectivity between coral populations in western Indonesia (Seribu Archipelago), central Indonesia (Spermonde Archipelago), and eastern Indonesia (Ambon). We sampled two readily identifiable corals popular in the marine aquarium trade, one species widely reported as a brooder (Euphyllia glabrescens), the other as a broadcast spawner (Lobophyllia corymbosa). The mitochondrial COI genome was amplified for 117 samples. Within-population genetic variation was high, especially at the eastern Indonesia (Ambon) site. The genetic connectivity patterns were similar for the two corals, with high connectivity between the Seribu and Spermonde Archipelagos (despite a geographical separation of more than 1,000 km) and a lack of connectivity between these two sites and Ambon. These results indicate a potential barrier to gene flow between coral populations in western/central Indonesia and those to the east of Sulawesi Island.</jats:p