Analysis of Expressed Sequence Tags (EST) Obtained from Common carp, Cyprinus carpio L., Head Kidney Cells After Stimulation by CpG oligodeoxynucleotides

Abstract

We analyzed genes expressed from head kidney of common carp Cyprinus carpio L. treated with CpG oligodeoxynucleotides. The results of single-pass sequencing of expressed sequence tags (ESTs) from 88 clones (AU312478-AU312561) from kidney cDNA are presented. Out of 88 clones 84 (95.5 %) matched with nucleic acid and/or amino acid sequences, whereas the remaining 4 (4.5 %) clones did not show any significant homology to the sequences in the databases. Immune related cDNA clones identified from kidney were granulin2, CCAAT/enhancer binding protein, immunoglobulin heavy chain variable region, lectin, lysozyme C, interleukin-4 receptor alpha chain, cathepsin L preproprotein, CD9 protein and Granulin 1 were identified