MRI role in the periprosthetic lymphoma screening: a case report

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Large Eddy Simulations of sediment entrainment induced by a lock-exchange gravity current

Large Eddy simulations of lock-exchange gravity currents propagating over a mobile reach are presented. The numerical setting allows to investigate the sediment pick up induced by the currents and to study the underlying mechanisms leading to sediment entrainment for different Grashof numbers and grain sizes. First, the velocity field and the bed shear-stress distribution are investigated, along with turbulent structures formed in the flow, before the current reaches the mobile bed. Then, during the propagation of the current above the erodible section of the bed the contour plots of the entrained material are pre- sented as well as the time evolution of the areas covered by the current and by the sediment at this section. The numerical outcomes are compared with experimental data showing a very good agreement. Overall, the study confirms that sediment pick up is prevalent at the head of the current where the strongest turbulence occurs. Further, above the mobile reach of the bed, settling process seems to be of minor importance, with the entrained material being advected downstream by the current. Additionally, the study shows that, although shear stress is the main mechanism that sets particles in motion, turbu- lent bursts as well as vertical velocity fluctuations are also necessary to counteract the falling velocity of the particles and maintain them into suspension. Finally, the analysis of the stability conditions of the current shows that, from one side, sediment concentration gives a negligible contribution to the stability of the front of the current and from the other side, the stability conditions provided by the current do not allow sediments to move into the ambient fluid

Surface and subsurface contributions to the build-up of forces on bed particles

In nature and in many industrial applications, the boundary of a channel flow is made of solid particles which form a porous wall, so that there is a mutual influence between the free flow and the subsurface flow developing inside the pores. While the influence of the porous wall on the free flow has been well studied, less well characterized is the subsurface flow, due to the practical difficulties in gathering information in the small spaces given by the pores. It is also not clear whether the subsurface flow can host turbulent events able to contribute significantly to the build-up of forces on the particles, potentially leading to their dislodgement. Through large eddy simulations, we investigate the interface between a free flow and a bed composed of spherical particles in a cubic arrangement. The communication between surface and subsurface flow is in this case enhanced, with relatively strong turbulent events happening also inside the pores. After comparing the simulation results with a previous experimental work from a similar setting, the forces experienced by the boundary particles are analysed. While it remains true that the lift forces are largely dependent on the structure of the free flow, turbulence inside the pores can also give a significant contribution. Pressure inside the pores is weakly correlated to the pressure in the free flow, and strong peaks above and below a particle can happen independently. Ignoring the porous layer below the particle from the computations leads then in this case to an underestimation of the lift forces

Detection of SF3B1 p.Lys700Glu Mutation by PNA-PCR Clamping in Myelodysplastic Syndromes and Myeloproliferative Neoplasms

Mutations in SF3B1 are found in 20% of myelodysplastic syndromes and 5–10% of myeloproliferative neoplasms, where they are considered important for diagnosis and therapy decisions. Sanger sequencing and NGS are the currently available methods to identify SF3B1 mutations, but both are time-consuming and expensive techniques that are not practicable in most small-/medium-sized laboratories. To identify the most frequent SF3B1 mutation, p.Lys700Glu, we developed a novel fast and cheap assay based on PNA-PCR clamping. After setting the optimal PCR conditions, the limit of detection of PNA-PCR clamping was evaluated, and the method allowed up to 0.1% of mutated SF3B1 to be identified. Successively, PNA-PCR clamping and Sanger sequencing were used to blind test 90 DNA from patients affected by myelodysplastic syndromes and myeloproliferative neoplasms for the SF3B1 p.Lys700Glu mutation. PNA-PCR clamping and Sanger sequencing congruently identified 75 negative and 13 positive patients. Two patients identified as positive by PNA-PCR clamping were missed by Sanger analysis. The discordant samples were analyzed by NGS, which confirmed the PNA-PCR clamping result, indicating that these samples contained the SF3B1 p.Lys700Glu mutation. This approach could easily increase the characterization of myelodysplastic syndromes and myeloproliferative neoplasms in small-/medium-sized laboratories, and guide patients towards more appropriate therapy

Large eddy simulation of stably stratified open channel flow

Large eddy simulation has been used to study flow in an open channel with stable stratification imposed at the free surface by a constant heat flux and an adiabatic bottom wall. This leads to a stable pycnocline overlying a well-mixed turbulent region near the bottom wall. The results are contrasted with studies in which the bottom heat flux is nonzero, a difference analogous to that between oceanic and atmospheric boundary layers. Increasing the friction Richardson number, a measure of the relative importance of the imposed surface stratification with respect to wall-generated turbulence, leads to a stronger, thicker pycnocline which eventually limits the impact of wall-generated turbulence on the free surface. Increasing stratification also leads to an increase in the pressure-driven mean streamwise velocity and a concomitant decrease in the skin friction coefficient, which is, however, smaller than in the previous channel flow studies where the bottom buoyancy flux was nonzero. It is found that the turbulence in any given region of the flow can be classified into three regimes (unstratified, buoyancy-affected, and buoyancy-dominated) based on the magnitude of the Ozmidov length scale relative to a vertical length characterizing the large scales of turbulence and to the Kolmogorov scale. Since stratification does not strongly influence the near-wall turbulent production in the present configuration, the behavior of the buoyancy flux, turbulent Prandtl number, and mixing efficiency is qualitatively different from that seen in stratified shear layers and in channel flow with fixed temperature walls, and, furthermore, collapse of quantities as a function of gradient Richardson number is not observed. The vertical Froude number is a better measure of stratified turbulence in the upper portion of the channel where buoyancy, by providing a potential energy barrier, primarily affects the transport of turbulent patches generated at the bottom wall. The characteristics of free-surface turbulence including the kinetic energy budget and pressure-strain correlations are examined and found to depend strongly on the surface stratification. (c) 2005 American Institute of Physics

The role of Pd over layer thickness on PLD YBCO coated conductor properties

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