5,555 research outputs found
Metodologia do isolamento de embrião zigótico de cajueiro-anão precoce in vitro.
bitstream/item/55341/1/COT11016.pd
Desenvolvimento de dot-blot para detecção de anticorpos para o vírus da Artrite Encefalite Caprina em caprinos.
Resumo: A técnica de imunodifusão em gel de ágar (IDGA) é empregada mundialmente como método de triagem e monitoramento das fases iniciais de programas de controle das lentiviroses de pequenos ruminantes, mas apesar da boa especificidade, a IDGA pode apresentar resultados falso-negativos. Este trabalho teve como objetivo padronizar o teste Dot-Blot (DB) para a detecção de anticorpos, em caprinos, contra o Lentivírus Caprino (LVC), utilizando antígeno experimental preparado a partir do vírus total, e compará-lo com a IDGA e com ELISA indireto (ELISA-i). Na realização do (DB) a membrana de nitrocelulose (MN) foi disposta num aparelho de blot de 96 poços acrescentando antígeno com uma concentração de 0,5 mg de proteína/poço. Colocaram-se as tiras de MN em tubos de ensaio contendo soro teste diluído (1:50). Após, distribuiu-se o conjugado (peroxidase IgG coelho anti-cabra) diluído 1:500 em PBS-T e revelou-se a MN numa solução de DAB/4-Cloronapthtol. Num total de 327 amostras verificou-se que o ELISA-i detectou 209 caprinos positivos, o DB detectou 200, enquanto a IDGA detectou 144 animais. O DB mostrou concordância de 90,2% (p<0,01) com o Elisa-i. O DB é um teste mais sensível que a IDGA e comparável ao ELISA-i, além de não necessitar da indumentária tecnológica do ELISA-i, podendo ser utilizado em eventos agropecuários ou até mesmo na propriedade. Summary: The imunodifusion in agar gel technique (IDGA) is used worldwide as a screening method of monitoring the initial phases of programs to control lentivirosis of small ruminants. Despite of the good specificity, the IDGA can present false-negative results. The objective of this work was to standardize the Dot-Blot test (DB) for the detention of antibodies against Lentivírus Caprine (LVC), using a prepared experimental antigen from the total virus. The DB was compared with the IDGA and indirect ELISA (ELISA-i) tests. The DB test was accomplished by used a nitrocelulose membrane (MN) in a device of blot of 96 wells and the antigen placed in a concentration of 0.5 mg of protein/well. The strips of MN placed in separated tubes and serum tests added at dilution of 1:50. After that, the conjugated peroxidase IgG rabbit anti-goat diluted 1:500 in PBS-T was incubated for 60 minutes. The DAB/4-Cloronapthtol solution was used to reveal the reaction. From a total of 327 samples, we verified that the ELISA-i detected 209 positives goat, the DB detected 200, while the IDGA 144 animals. The DB showed agreement of 90.2% (p<0.01) with the Elisa-i. The DB is a test more viable than the IDGA and comparable to the ELISA-i test. Besides being more sensible than the IDGA, it does not need the technological equipment of the ELISA-i, being able to be used in animals for events or in the field
Robust exponential decay of correlations for singular-flows
We construct open sets of Ck (k bigger or equal to 2) vector fields with
singularities that have robust exponential decay of correlations with respect
to the unique physical measure. In particular we prove that the geometric
Lorenz attractor has exponential decay of correlations with respect to the
unique physical measure.Comment: Final version accepted for publication with added corrections (not in
official published version) after O. Butterley pointed out to the authors
that the last estimate in the argument in Subsection 4.2.3 of the previous
version is not enough to guarantee the uniform non-integrability condition
claimed. We have modified the argument and present it here in the same
Subsection. 3 figures, 34 page
Periodic Neural Activity Induced by Network Complexity
We study a model for neural activity on the small-world topology of Watts and
Strogatz and on the scale-free topology of Barab\'asi and Albert. We find that
the topology of the network connections may spontaneously induce periodic
neural activity, contrasting with chaotic neural activities exhibited by
regular topologies. Periodic activity exists only for relatively small networks
and occurs with higher probability when the rewiring probability is larger. The
average length of the periods increases with the square root of the network
size.Comment: 4 pages, 5 figure
Efeito de banhos terapêuticos com formalina sobre indicadores de estresse em tambaqui.
O objetivo deste trabalho foi avaliar o efeito de concentrações terapêuticas de formalina na homeostase de tambaqui (Colossoma macropomum Cuvier 1818) por meio de índices fisiológicos
- …