Epilithic biomass in a large gravel-bed river (the Garonne, France): a manifestation of eutrophication?

In order to evaluate the impact of outputs of the city of Toulouse (740 000 inhabitants) on the epilithic communities colonizing pebble banks in the river Garonne, a large gravel-bed river (eighth order), dry mass (DM), ash-free dry mass (AFDM) and chlorophyll-a (chla) epilithic biomass per unit area were measured and autotrophic index (AI) (i.e. ratio AFDM/chla) was calculated at four stations. This river is morphologically characterized by a succession of pools and riffles and by highly fluctuating hydraulic conditions. At the four stations studied (223 km apart), the means of AFDM values varied between 17.1 and 31.1 g m−2 of colonized surface and the chla concentration varied between 112 and 254 mg m−2. However, there were no significant differences in AFDM per unit area between the parts of the river upstream and downstream of the Toulouse area (Mann–Whitney U-test statistic), nor between the four stations (Kruskal–Wallis test statistic), and the AI did not allow the description of changes in periphyton communities between sampling locations. This study showed that epilithic biomass should be considered as the typical microbial community of the river rather than as a manifestation of eutrophication