Mechanosensing is critical for axon growth in the developing brain.

During nervous system development, neurons extend axons along well-defined pathways. The current understanding of axon pathfinding is based mainly on chemical signaling. However, growing neurons interact not only chemically but also mechanically with their environment. Here we identify mechanical signals as important regulators of axon pathfinding. In vitro, substrate stiffness determined growth patterns of Xenopus retinal ganglion cell axons. In vivo atomic force microscopy revealed a noticeable pattern of stiffness gradients in the embryonic brain. Retinal ganglion cell axons grew toward softer tissue, which was reproduced in vitro in the absence of chemical gradients. To test the importance of mechanical signals for axon growth in vivo, we altered brain stiffness, blocked mechanotransduction pharmacologically and knocked down the mechanosensitive ion channel piezo1. All treatments resulted in aberrant axonal growth and pathfinding errors, suggesting that local tissue stiffness, read out by mechanosensitive ion channels, is critically involved in instructing neuronal growth in vivo.This work was supported by the German National Academic Foundation (scholarship to D.E.K.), Wellcome Trust and Cambridge Trusts (scholarships to A.J.T.), Winston Churchill Foundation of the United States (scholarship to S.K.F.), Herchel Smith Foundation (Research Studentship to S.K.F.), CNPq 307333/2013-2 (L.d.F.C.), NAP-PRP-USP and FAPESP 11/50761-2 (L.d.F.C.), UK EPSRC BT grant (J.G.), Wellcome Trust WT085314 and the European Research Council 322817 grants (C.E.H.); an Alexander von Humboldt Foundation Feodor Lynen Fellowship (K.F.), UK BBSRC grant BB/M021394/1 (K.F.), the Human Frontier Science Program Young Investigator Grant RGY0074/2013 (K.F.), the UK Medical Research Council Career Development Award G1100312/1 (K.F.) and the Eunice Kennedy Shriver National Institute Of Child Health & Human Development of the National Institutes of Health under Award Number R21HD080585 (K.F.).This is the author accepted manuscript. The final version is available from Nature Publishing Group via https://doi.org/10.1038/nn.439

Contractor Drilling with Pounder II

Project Manager Dr Richard Carter, Institute of Water and Environment, Cranfield University at Silsoe.This report contributes to the findings, implications, and future plans of a project, initiated by Cranfield University (Silsoe, UK) entitled “Private Sector Participation in Low Cost Water Well Drilling”.The project was funded by DFID from July 1998 to June 2001, with additional funding partners (Government of Uganda, DANIDA, SIDA, UNICEF, Water Aid, and an anonymous donor) joining at various stages throughout this three-year period

Indigenous psychology in Aotearoa/New Zealand and Australia

In Aotearoa/New Zealand and Australia, the development of Indigenous psychology is a response to the resilience of a colonised people, where the gaze of Western imperialism is ever present. The use of esoteric, ceremonial, environmental, and relational knowledge is included to counter balance the individualism inherent in mainstream psychology. Across both countries, connections to ancestors, land, language, customs and relationships are important. Dudgeon’s Social and Emotional Wellbeing model offers a transformative lens for addressing the significant disparities that Aboriginal and Torres Strait Islander peoples’ experience. While Māori wellbeing includes healthy relationships between physical, psychological, community, spirituality and environment domains. The chapter promotes a reclamation of Indigenous knowledge systems that, if not protected and promoted, could be lost from their cultural home