Ethnobotanical and ethnopharmacological studies of medicinal plants used in rural areas of Kavrepalanchok District (Central Nepal).

The current economic and social conditions in many rural areas of the world are threatening the precious heritage of ethnobotanical knowledge and traditional farming practices.This can cause loss of precious cultural heritage and reduction in plant biodiversity, as ancient crops tend to disappear. The main aim of this thesis is to document traditional uses of plants by different ethnic groups (Tibeto-Burman and Indo-Aryan) living in certain rural areas of the Kavrepalanchok District in Central Nepal. In the study area, due to distance from urban centres and difficulty in accessing the government healthcare system, people still rely heavily on the use of local plants for various purposes, above all for primary healthcare. Through interviews with 32 informants, most of whom were key informants, we explored uses of 116 plant species, of which 101 were plants with medicinal value employed in the treatment of human and veterinary diseases. Some unusual uses of medicinal plants and original recipes were also reported. The data document the richness of the local flora and traditional knowledge of medicinal plant species used by ethnic communities in these rural areas. Therefore, future projects will have to involve local people in the improvement and conservation of the biological and cultural heritage.There is also a need for an ecological strategy for integrated management of land, water and living resources. Another aim of the research presented in this thesis is to better characterise some plants found to be of particular interest among those surveyed in the study area. With this in mind, we have focused our attention on those plants used by informants in the treatment of nervous system disorders, such as two species belonging to Caprifoliaceae (formerly Valerianaceae): Valeriana jatamansi Jones ex Rob. and Nardostachys jatamansi (D. Don) DC. These plants are widely used in traditional medicine for their sedative and anxiolytic properties in Nepal and in many other Asian countries. The pharmacognostic and phytochemical profile and the biological effects of essential oils (EOs) of these species were compared with those of Valeriana officinalis L., a species whose phytotherapeutic use is widespread in Western medicine. The multidisciplinary approach used represents a way to avoid adulteration of herbal drugs and allows evaluation of the effectiveness of EOs that could be used for a wide range of therapeutic applications. Overall, the results of this research could be useful for enhancing knowledge of the potential of still little-known medicinal plants for the possible formulation of new pharmaceutical products, eventually contributing to the economic development of local communities

An integrated approach to characterize the dynamic behaviour of a mechanical chain tensioner by functional tolerancing

Assembled systems composed of flexible components are widely used in mechanics to dampen vibrations and store or dissipate energy. Often, the flexible components of these systems are assembled via non-linear sliding contacts and yielding constraints. Geometric non-linearity along with non-linearity of stiffness, damping and contact pressure between flexible components greatly complicate the dynamic characterization of these assemblies. Therefore, such assemblies are characterised almost exclusively by means of experimental testing. This research analyses how classic ASME and ISO tolerance standards can be used to guarantee and control the conformity of these assembled systems with their functional requirements limiting the number of experimental tests. In particular the dependence of the dynamic behaviour upon functional tolerances is studied for a mechanical tensioner in a chain drive timing system of an internal combustion engine (ICE). The semi empirical methodology is based on displacement measurements and modal analyses. A multibody model with few degrees of freedom (MBM-FDoF) is proposed as the first approximation to reproduce the variability of the dynamic behaviour of the tensioner considering variations in functional tolerances

Characterization of Ingredients Incorporated in the Traditional Mixed-Salad of the Capuchin Monks

Recipes on the composition of the “salad of the monks” (Capuchin monks) have been reported in Italy since the 17th century. Different wild edible plants were highly regarded as an important ingredient of this mixed salad. Among these, some species played a key role for both their taste and nutritional properties: Plantago coronopus L. (PC), Rumex acetosa L., Cichorium intybus L., and Artemisia dracunculus L. In the present study, the micromorphological and phytochemical features as well as the antioxidant and anti-inflammatory properties of extracts of these fresh and blanched leaves, were investigated. The extracts obtained by blanched leaves, according to the traditionally used cooking method, showed the highest content of bioactive compounds (total phenols 1202.31–10,751.88 mg GAE/100 g DW; flavonoids 2921.38–61,141.83 mg QE/100 g DW; flavanols 17.47–685.52 mg CE/100 g DW; proanthocyanidins 2.83–16.33 mg CyE/100 g DW; total chlorophyll 0.84–1.09 mg/g FW; carbohydrates 0.14–1.92 g/100 g FW) and possess the most marked antioxidant (IC50 0.30–425.20 µg/mL) and anti-inflammatory activity (IC50 240.20–970.02 µg/mL). Considering this, our results indicate that increased consumption of the investigated plants, in particular of PC, raw or cooked briefly, could provide a healthy food source in the modern diet by the recovery and enhancement of ancient ingredients

Comparative and Functional Screening of Three Species Traditionally used as Antidepressants: Valeriana officinalis L., Valeriana jatamansi Jones ex Roxb. and Nardostachys jatamansi (D.Don) DC.

The essential oils (EOs) of three Caprifoliaceae species, the Eurasiatic Valeriana officinalis (Vo), the Himalayan Valeriana jatamansi (Vj) and Nardostachys jatamansi (Nj), are traditionally used to treat neurological disorders. Roots/rhizomes micromorphology, DNA barcoding and EOs phytochemical characterization were carried out, while biological effects on the nervous system were assessed by acetylcholinesterase (AChE) inhibitory activity and microelectrode arrays (MEA). Nj showed the highest inhibitory activity on AChE (IC50 67.15 μg/mL) followed by Vo (IC50 127.30 μg/mL) and Vj (IC50 246.84 μg/mL). MEA analyses on rat cortical neurons, carried out by recording mean firing rate (MFR) and mean bursting rate (MBR), revealed stronger inhibition by Nj (IC50 18.8 and 11.1 μg/mL) and Vo (16.5 and 22.5 μg/mL), compared with Vj (68.5 and 89.3 μg/mL). These results could be related to different EO compositions, since sesquiterpenes and monoterpenes significantly contribute to the observed effects, but the presence of oxygenated compounds such as aldehydes and ketones is a discriminating factor in determining the order of potency. Our multidisciplinary approach represents an important tool to avoid the adulteration of herbal drugs and permits the evaluation of the effectiveness of EOs that could be used for a wide range of therapeutic applications

Practice guidelines for the treatment of hepatitis C: recommendations from an AISF/SIMIT/SIMAST Expert Opinion Meeting

It is increasingly clear that a tailored therapeutic approach to patients with hepatitis C virus infection is needed. Success rates in difficult to treat and low-responsive hepatitis C virus patients are not completely satisfactory, and there is the need to optimise treatment duration and intensity in patients with the highest likelihood of response. In addition, the management of special patient categories originally excluded from phase III registration trials needs to be critically re-evaluated. This article reports the recommendations for the treatment of hepatitis C virus infection on an individual basis, drafted by experts of three scientific societies

Practice guidelines for the treatment of hepatitis C: recommendations from an AISF/SIMIT/SIMAST Expert Opinion Meeting.

It is increasingly clear that a tailored therapeutic approach to patients with hepatitis C virus infection is needed. Success rates in difficult to treat and low-responsive hepatitis C virus patients are not completely satisfactory, and there is the need to optimise treatment duration and intensity in patients with the highest likelihood of response. In addition, the management of special patient categories originally excluded from phase III registration trials needs to be critically re-evaluated. This article reports the recommendations for the treatment of hepatitis C virus infection on an individual basis, drafted by experts of three scientific societies