Comparative study of single and multislice computed tomography for assessment of the mandibular canal

OBJECTIVE: The purpose of this study was to evaluate the accuracy of relative measurements from the roof of the mandibular canal to the alveolar crest in multislice (multidetector) computed tomography (MDCT) and single-slice computed tomography (SSCT). MATERIAL AND METHODS: The sample consisted of 26 printed CT films (7 SSCT and 19 MDCT) from the files of the LABI-3D (3D Imaging Laboratory) of the School of Dentistry of the University of São Paulo (FOUSP), which had been acquired using different protocols. Two observers analyzed in a randomized and independent order a series of 22 oblique CT reconstructions of each patient. Each observer analyzed the CT scans twice. The length of the mandibular canal and the distance between the mandibular canal roof and the crest of the alveolar ridge were obtained. Dahlberg test was used for statistical analysis. RESULTS: The mean error found for the mandibular canal length measurements obtained from SSCT was 0.53 mm in the interobserver analysis, and 0.38 mm for both observers. On MDCT images, the mean error was 0.0 mm in the interobserver analysis, and 0.0 and 0.23 mm in the intraobserver analysis. Regarding the distance between the mandibular canal roof and the alveolar bone crest, the SSCT images showed a mean error of 1.16 mm in the interobserver analysis and 0.66 and 0.59 mm in the intraobserver analysis. In the MDCT images, the mean error was 0.72 mm in the interobserver analysis and 0.50 and 0.54 mm in the intraobserver analysis. CONCLUSION: Multislice CT was demonstrated a more accurate method and demonstrated high reproducibility in the analysis of important anatomical landmarks for planning of mandibular dental implants, namely the mandibular canal pathway and alveolar crest height

Isothermal Recombinase Polymerase amplification (RPA) of Schistosoma haematobium DNA and oligochromatographic lateral flow detection

© 2015 Rosser et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. The attached file is the published version of the article

DIAGNÓSTICOS DE ENFERMAGEM COMO INSTRUMENTOS NA FORMAÇÃO DO ENFERMEIRO: UMA REVISÃO DE LITERATURA

This research presents a review of articles on the topic of "Nursing Diagnosis," published during the years 2000-2007 and refers to a trend which has increasingly been mentioned in practice and in medical journals: The study is based on evidence. To this end, we used the methodology of a bibliographic search, using data sources such as the national nursing journals indexed in the LILACS database (Latin-American and Caribbean Health Sciences Literature), MEDLINE (International Science Literature Health) SciELO (Scientific Electronic Library Online). We chose to use the term "Nursing Diagnosis," that was found in 65 articles. For the analysis it was revealed the nature of the items, the assessment of the authors and how they evaluated the diagnosis teaching of nursing. It highlighted the main theoretical references, issues of proportionality and the years of the publication in question. It can be concluded that the production of nursing articles, within the theme of the diagnosis, has been made even in small quantities, which indicates the need of production and application of these articles in the fields of teaching and practice.  Esta investigación presenta una revisión bibliográfica de los artículos sobre el tema "Diagnóstico de Enfermería", publicados durante los años 2000-2007, y se refiere a una tendencia que se ha afirmado en la práctica y en las revistas médicas: el estudio basado en la evidencia. Con este fin, hemos utilizado la metodología de búsqueda bibliográfica, utilizando fuentes de datos como las revistas de enfermería nacional indexadas a la base de datos LILACS (Literatura Latino-Americana y del Caribe en Ciencias de la Salud), MEDLINE (Literatura Internacional en Ciencias Salud) y SCIELO (Scientific Electronic Library Online). Se optó por utilizar el término "Diagnóstico de Enfermería", donde fueron encontrados 65 artículos. Para el análisis se puso de manifiesto la naturaleza de los artículos, la valoración de los autores, y cómo estos evalúan el diagnóstico en el hacer/educar en enfermería. Se destacaron los principales teóricos referenciados, temas y proporcionalidad de los años de la publicación en cuestión. Se puede concluir que la producción de artículos de enfermería, dentro de la temática del diagnóstico, se ha hecho incluso en pequeñas cantidades, lo que indica la necesidad de la producción y aplicación de estos productos en las esferas de la enseñanza y la práctica, teniendo en cuenta la consideración de enfermería como ciencia.Esta pesquisa apresenta uma revisão de literatura dos artigos referentes ao tema “Diagnósticos de Enfermagem” publicados no período dos anos 2000-2007, e relaciona-os a uma tendência, a qual cada vez tem se afirmado na prática e nas publicações médicas: o estudo baseado em evidências. Para tal, utilizou-se a metodologia de pesquisa bibliográfica, utilizando-se como fontes de dados as Revistas de Enfermagem nacionais indexadas às base de dados LILACS (Literatura Latino-Americana e do Caribe em Ciências da Saúde), MEDLINE (Literatura Internacional em Ciências da Saúde) e Scielo (Scientific Electronic Library Online). Utilizando como descritor o termo “Diagnósticos de Enfermagem”, foram encontrados 65 artigos. Para a análise demonstramos a natureza dos artigos, a titulação dos autores, e como estes avaliam a visão diagnóstica dentro do fazer/ educar na Enfermagem, procuramos evidenciar os principais teóricos referenciados, temáticas e proporcionalidade dos anos de publicação em questão. Pode-se concluir que a produção de artigos na Enfermagem, dentro da temática diagnóstico, tem-se apresentado ainda em pequena quantidade, evidenciando a necessidade de produção e aplicabilidade destas produções, nos campos da docência e prática, visto a afirmação da Enfermagem como ciência.  

Dependence of cancer cell adhesion kinetics on integrin ligand surface density measured by a high-throughput label-free resonant waveguide grating biosensor

A novel high-throughput label-free resonant waveguide grating (RWG) imager biosensor, the Epic® BenchTop (BT), was utilized to determine the dependence of cell spreading kinetics on the average surface density (vRGD) of integrin ligand RGD-motifs. vRGD was tuned over four orders of magnitude by co-adsorbing the biologically inactive PLL-g-PEG and the RGD-functionalized PLL-g-PEG-RGD synthetic copolymers from their mixed solutions onto the sensor surface. Using highly adherent human cervical tumor (HeLa) cells as a model system, cell adhesion kinetic data of unprecedented quality were obtained. Spreading kinetics were fitted with the logistic equation to obtain the spreading rate constant (r) and the maximum biosensor response (Δλmax), which is assumed to be directly proportional to the maximum spread contact area (Amax). r was found to be independent of the surface density of integrin ligands. In contrast, Δλmax increased with increasing RGD surface density until saturation at high densities. Interpreting the latter behavior with a simple kinetic mass action model, a 2D dissociation constant of 1753 ± 243 μm−2 (corresponding to a 3D dissociation constant of ~30 μM) was obtained for the binding between RGD-specific integrins embedded in the cell membrane and PLL-g-PEG-RGD. All of these results were obtained completely noninvasively without using any labels

A virtual reality and retailing literature review: Current focus, underlying themes and future directions

This literature review reveals the current research focus, underlying themes and prominent research gaps in the Virtual Reality (VR) literature. 89 journal articles from the 22 years are thematically analysed in order to non-obvious reveal interconnections and themes, including research focus over time and underlying themes by research discipline. Over half of all papers focus on the need to understand the VR shopping consumer, yet no consensus exists as to what the optimal experience is or how to design effective v-Commerce stores. The most prominent research gaps are related to the unique HCI aspects in v-Commerce that influence shopping behaviours. The impact of this review is establishing the current challenges and future directions for academia in order to make v-Commerce a viable reality. Specifically, future research should focus on develop human factor theory in VR shop design (i.e. social dimension, eye-tracking etc.)

Serum biomarkers associated with SARS-CoV-2 severity

Immunity with SARS-CoV-2 infection during the acute phase is not sufficiently well understood to differentiate mild from severe cases and identify prognostic markers. We evaluated the immune response profile using a total of 71 biomarkers in sera from patients with SARS-CoV-2 infection, confirmed by RT-PCR and controls. We correlated biological marker levels with negative control (C) asymptomatic (A), nonhospitalized (mild cases-M), and hospitalized (severe cases-S) groups. Among angiogenesis markers, we identified biomarkers that were more frequently elevated in severe cases when compared to the other groups (C, A, and M). Among cardiovascular diseases, there were biomarkers with differences between the groups, with D-dimer, GDF-15, and sICAM-1 higher in the S group. The levels of the biomarkers Myoglobin and P-Selectin were lower among patients in group M compared to those in groups S and A. Important differences in cytokines and chemokines according to the clinical course were identified. Severe cases presented altered levels when compared to group C. This study helps to characterize biological markers related to angiogenesis, growth factors, heart disease, and cytokine/chemokine production in individuals infected with SARS-CoV-2, offering prognostic signatures and a basis for understanding the biological factors in disease severity