Possible international directive for quality control of bee pollen

Comunicação oral da qual só está disponível a apresentação.Possible international directive for quality control of bee pollen.Fundação Ciência e Tecnologia de Portugal POCTI (FEDER) & COMPETE, Ciência Viv

Chemical analysis of Greek pollen - Antioxidant, antimicrobial and proteasome activation properties

<p>Abstract</p> <p>Background</p> <p>Pollen is a bee-product known for its medical properties from ancient times. In our days is increasingly used as health food supplement and especially as a tonic primarily with appeal to the elderly to ameliorate the effects of ageing. In order to evaluate the chemical composition and the biological activity of Greek pollen which has never been studied before, one sample with identified botanical origin from sixteen different common plant taxa of Greece has been evaluated.</p> <p>Results</p> <p>Three different extracts of the studied sample of Greek pollen, have been tested, in whether could induce proteasome activities in human fibroblasts. The water extract was found to induce a highly proteasome activity, showing interesting antioxidant properties. Due to this activity the aqueous extract was further subjected to chemical analysis and seven flavonoids have been isolated and identified by modern spectral means. From the methanolic extract, sugars, lipid acids, phenolic acids and their esters have been also identified, which mainly participate to the biosynthetic pathway of pollen phenolics. The total phenolics were estimated with the Folin-Ciocalteau reagent and the total antioxidant activity was determined by the DPPH method while the extracts and the isolated compounds were also tested for their antimicrobial activity by the dilution technique.</p> <p>Conclusions</p> <p>The Greek pollen is rich in flavonoids and phenolic acids which indicate the observed free radical scavenging activity, the effects of pollen on human fibroblasts and the interesting antimicrobial profile.</p

The Botanical Profiles of Dried Bee Pollen Loads Collected by Apis mellifera (Linnaeus) in Brazil

Submitted by sandra infurna ([email protected]) on 2016-02-02T11:32:02Z No. of bitstreams: 1 monica4_barth_etal_IOC_2013.pdf: 570692 bytes, checksum: 7c7ae59dfe217b3cc6d0f0a3c21c58d8 (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-02-02T11:39:21Z (GMT) No. of bitstreams: 1 monica4_barth_etal_IOC_2013.pdf: 570692 bytes, checksum: 7c7ae59dfe217b3cc6d0f0a3c21c58d8 (MD5)Made available in DSpace on 2016-02-02T11:39:21Z (GMT). No. of bitstreams: 1 monica4_barth_etal_IOC_2013.pdf: 570692 bytes, checksum: 7c7ae59dfe217b3cc6d0f0a3c21c58d8 (MD5) Previous issue date: 2013Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Universidade de São Paulo. São Paulo, SP, Brasil.Universidade de São Paulo. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.A total of 61 dried bee pollen samples collected in four Brazilian macro-regions within 19 municipalities were analyzed aiming to identify the sources used by Apis mellifera (L.) for pollen production and to enable a more accurate product certification. Sample preparation followed the standard methodology, including washing the pollen grains with ethanol, then with water and homogenising the sediment in a water/glycerine solution for microscopic observation. Pollen counts included at least 500 pollen grains per sample. Only six samples, presenting a unique species or pollen type comprising more than 90% of the pollen sum, were considered monofloral, including Ambrosia sp., Cecropia sp., Eucalyptus sp., Fabaceae, Mimosa scabrella (Benth.) spp. and Schinus sp. pollen types. The most frequent pollen types of the heterofloral pollen batches, based on a counting limit of 45%, included Anadenanthera sp., Asteraceae, Brassica sp., Caesalpiniaceae, Cocos nucifera (L.) sp., Mimosa caesalpiniaefolia (Benth.) sp., Mimosa verrucosa (Benth.) sp., and Myrcia sp. pollen types. This result may be related to the great diversity of the Brazilian flora contributing to heterofloral pollen loads and honeys

Optimization of thermogravimetric analysis of ash content in honey

A factorial design to evaluate ash content in honey by thermogravimetry as well as to establish the optimum analysis conditions that produce ash values in agreement with those obtained by the official method (gravimetry) was executed. Heating rate and use of fiber glass paper effects were significant as was the interaction effect between them. Lower heating rates and the use of fiber glass fiber paper are recommended to provide the complete incineration of honey samples by thermogravimetry. To confirm these results and to establish the optimized conditions a completely randomized two block design was executed investigating three procedures: thermogravimetry with fiber glass paper at heating rates of 5 and 10 ºC min-1 and standard gravimetry. Only the thermogravimetric method carried out at 5 &deg;C min-1 presented ash content averages in agreement with those obtained by the official method. In this way, TG/DTG can be indicated as an alternative method to gravimetry for this determination in honey samples

Optimization of mobile phase for separation of carbohydrates in honey by high performance liquid chromatography using a mixture design

A mixture design was used to optimize the mobile phase composition for separation of carbohydrates in honey by High Performance Liquid Chromatography. Nine ternary acetronitrile, water and ethyl acetate mobile phases corresponding to a simplex centroid design with axial points were test to separate those carbohydrates most frequently encountered in honey samples. The results suggest that a special cubic model accurately describes changes in the proportions of these solvents in the mobile phase close to the region of optimal peak separation. The addition of a third solvent, ethyl acetate, to binary mobile phase of water and acetonitrile permitted a significant improvement in carbohydrate separation that can be applied to the quantitative analysis of these compounds in honey.Um planejamento de misturas foi usado para otimizar a composição da fase móvel para a separação dos carboidratos em mel por Cromatografia Líquida de Alta Eficiência. Nove fases móveis ternárias de acetonitrila, água e acetato de etila, correspondendo a um planejamento centróide simplex com pontos axiais foram testadas para separar os carboidratos mais freqüentemente encontrados em amostras de mel. Os resultados sugerem que um modelo cúbico especial descreve precisamente as mudanças nas proporções destes solventes na fase móvel próxima a região ótima da separação dos picos. A adição de um terceiro solvente, acetato de etila, à fase móvel binária de água e acetonitrila permitiu uma melhora significativa na separação dos carboidratos que pode ser aplicada à análise quantitativa destes compostos em mel.58859