Particulate carriers as immunological adjuvants

In recent years, much interest has focused on the significance of inducing not only systemic immunity but also good local immunity at susceptible mucosal surfaces. A new field of mucosal immunity has been established as information accumulates on gut-associated lymphoid tissue, bronchus-associated lymphoid tissue and nasal-associated lymphoid tissue (GALT, BALT and NALT, respectively) and on their role in both local and systemic immune responses. This project, following the line of investigation started by other workers, was designed to study the use of microspheres to deliver antigens by the mucosal routes (oral and nasal). Antigen-containing microspheres were prepared with PLA and PLGA, by either entrapment within the particles or adsorption onto the surface. The model protein antigens used in this work were mainly tetanus toxoid (TT), bovine serum albumin (BSA) and γ-globulins.In vitro investigations included the study of physicochemical properties of the particulate carriers as well as the assessment of stability of the antigen molecules throughout the formulation procedures. Good loading efficiencies were obtained with both formulation techniques, which did not affect the immunogenicity of the antigens studied. The influence of the surfactant employed on the microspheres' surface properties was demonstrated as well as its implications on the adsorption of proteins. Preparations containing protein adsorbed were shown to be slightly more hydrophobic than empty PLA microspheres, which can enhance the uptake of particles by the antigen presenting cells that prefer to associate with hydrophobic surfaces. Systemic and mucosal immune responses induced upon nasal, oral and intramuscular administration have been assessed and, when appropriate, compared with the most widely used vaccine adjuvant, aluminium hydroxide. The results indicate that association of TT with PLA microspheres through microencapsulation or adsorption procedures led to an enhancement of specific mucosal IgA and IgG and systemic IgG responses to the mucosal delivered antigens. Particularly, nasal administration of TT produced significantly higher serum levels of specific IgG in test animals, as compared to control groups, suggesting that this is a potential route for vaccination. This implies the uptake and transfer of particles through the nasal mucosa, which was further demonstrated by the presence in the blood stream of latex particles as early as 10 min after nasal administration

Slowly synchronizing automata and digraphs

We present several infinite series of synchronizing automata for which the minimum length of reset words is close to the square of the number of states. These automata are closely related to primitive digraphs with large exponent.Comment: 13 pages, 5 figure

Can a restocking event with European (glass) eels cause early changes in local biological communities and its ecological status?

In an attempt to assist the recovery of the panmictic population of the European eel, declining since the late 1980s, the restocking of areas with low or no natural recruitment has been one of the measures adopted to reverse this trend. However, the main focus in several monitoring programmes for these actions, has been in the best interest of its viability and cost/benefit relationships and, for that, the condition of the released stocks has been the main concern. Yet, so far, no studies have assessed the potential ecological impacts that restocking might have on other biological communities. This pioneer pilot study aimed to evaluate the early ecological impact of a restocking event on other biological communities, considering inter-specific competition (other fish species) and feeding impact (macroinvertebrates).The reference condition of the biological communities of an inland tributary of the Mondego river, the River Ceira, was determined in three sites inaccessible to the natural recruitment of eels, followed by a post-stocking assessment. The results showed no significant changes in the fish assemblages in restocked areas, contrary to the macroinvertebrate community. However, the ecological status for the macroinvertebrate community showed no deleterious effects, with the results suggesting exactly the opposite. This may be related to the low density of the restocked eels and factors influencing the local trophic web. This study confirms the suitability of the habitat for restocking with glass eels, during its early stages, without disrupting the local ecological status, using densities close to those of natural recruitment

Pair production of the heavy leptons in future high energy linear e^{+}e^{-} colliders

The littlest Higgs model with T-parity predicts the existence of the T-odd particles, which can only be produced in pair. We consider pair production of the T-odd leptons in future high energy linear $e^{+}e^{-}$ collider ($ILC$). Our numerical results show that, as long as the T-odd leptons are not too heavy, they can be copiously produced and their possible signals might be detected via the processes $e^{+}e^{-}\to \bar{L}_{i}L_{j}$ in future $ILC$ experiments.Comment: Discussions added, typos and references correcte

Evaluation of Antioxidant Parameters in Rats Treated with Sevoflurane

SummaryBackground and objectivesSevoflurane is a halogenated fluorinated ether that undergoes hepatic biotransformation through cytochrome P4502E1. Halogenated ethers undergoing biotransformation by P4502E1 can produce reactive oxygen species (ROS), weakening the antioxidant defense mechanism. The objective of this study was to investigate the relationship between the activity of erythrocyte antioxidant enzymes and sevoflurane.MethodsAnimals were divided in four groups: Group 1 – control: 100% oxygen (1 L.min-1 for 60 min during five consecutive days); Group 2 – 4.0% sevoflurane in 100% oxygen (1 L.min-1 for 60 minutes during five consecutive days); Group 3 – isoniazid (i.p.), 50 mg.kg-1/day for four consecutive days, followed by 100% oxygen (1 L.min-1 for 60 minutes during four consecutive days); Group 4 – intraperitoneal isoniazid, 50 mg.kg-1 daily for four days, followed by 4.0% sevoflurane in 100% oxygen (1 L.min-1 for 60 minutes during five days). Twelve hours after the last exposure to sevoflurane, animals were sacrificed and their blood was collected through the portal vein for analysis of antioxidant enzymes.ResultsAn increase in the activity of glucose-6-phosphate dehydrogenase and a decrease in the activity of catalase were observed, especially in the group of animals pre-treated with isoniazid. Changes in the activity of glutathione peroxidase were not observed.ConclusionsThe interaction between sevoflurane and cytochrome P450 2E1 with enzymatic inducers can lead to oxidative stress with prolonged and repetitive exposure

Equivariant cohomology over Lie groupoids and Lie-Rinehart algebras

Using the language and terminology of relative homological algebra, in particular that of derived functors, we introduce equivariant cohomology over a general Lie-Rinehart algebra and equivariant de Rham cohomology over a locally trivial Lie groupoid in terms of suitably defined monads (also known as triples) and the associated standard constructions. This extends a characterization of equivariant de Rham cohomology in terms of derived functors developed earlier for the special case where the Lie groupoid is an ordinary Lie group, viewed as a Lie groupoid with a single object; in that theory over a Lie group, the ordinary Bott-Dupont-Shulman-Stasheff complex arises as an a posteriori object. We prove that, given a locally trivial Lie groupoid G and a smooth G-manifold f over the space B of objects of G, the resulting G-equivariant de Rham theory of f boils down to the ordinary equivariant de Rham theory of a vertex manifold relative to the corresponding vertex group, for any vertex in the space B of objects of G; this implies that the equivariant de Rham cohomology introduced here coincides with the stack de Rham cohomology of the associated transformation groupoid whence this stack de Rham cohomology can be characterized as a relative derived functor. We introduce a notion of cone on a Lie-Rinehart algebra and in particular that of cone on a Lie algebroid. This cone is an indispensable tool for the description of the requisite monads.Comment: 47 page

Travelling waves for the Gross-Pitaevskii equation II

The purpose of this paper is to provide a rigorous mathematical proof of the existence of travelling wave solutions to the Gross-Pitaevskii equation in dimensions two and three. Our arguments, based on minimization under constraints, yield a full branch of solutions, and extend earlier results, where only a part of the branch was built. In dimension three, we also show that there are no travelling wave solutions of small energy.Comment: Final version accepted for publication in Communications in Mathematical Physics with a few minor corrections and added remark

Quinze anos de monitorização das comunidades biológicas do estuário do Tejo no âmbito da construção e funcionamento das ETARs do Portinho da Costa e da Mutela (Concelho de Almada): Experiência adquirida e ensinamentos para futuros casos semelhantes.

Desde 2001 que a Câmara Municipal de Almada tem vindo a promover, em colaboração com o MARE, a monitorização ambiental das regiões estuarinas sob influência das ETARs do Portinho da Costa e da Mutela, que vieram substituir efluentes não tratados emitidos sensivelmente nas mesmas regiões. O objetivo deste programa de monitorização consiste em avaliar as principais alterações ocorridas (na maioria dos casos positivas) nos ecossistemas estuarinos dos vários locais, após a entrada em funcionamento de ambas as infraestruturas. Estes 15 anos de estudos das comunidades bentónicas e de peixes locais têm permitido identificar variações nos padrões espácio-temporais destes compartimentos biológicos e identificar quais dessas evoluções podem ser presumivelmente relacionáveis com o funcionamento das infraestruturas e quais derivam provavelmente de flutuações naturais. Nesta comunicação são enunciados os pressupostos do estudo, descritos os delineamentos experimentais e metodologias de tratamento dos dados adotados, apresentados os principais resultados obtidos e discutidas as grandes dificuldades sentidas para cumprir os objetivos estabelecidos. Este conjunto de elementos será analisado numa perspetiva de como a experiência adquirida pode ser útil, no futuro, para casos semelhantes, sobretudo numa ótica da correta identificação dos verdadeiros efeitos infraestruturais e de despiste da influência de outros fatores concorrentes

What does the nonextensive parameter q stand for in self-gravitating systems ?

It is natural important question for us to ask what the nonextensive parameter stands for when Tsallis statistics is applied to the self-gravitating systems. In this paper, some properties of the nonextensive parameter and Tsallis equilibrium distribution for the self-gravitating system are discussed in the framework of nonextensive kinetic theory. On the basis of the solid mathematical foundation, the nonextensive parameter can be expressed by a formula with temperature gradient and the gravitational potential and can be presented the physical meaning with regard to the non-isothermal (nonequilibrium stationary state) nature of the systems with long-range interactions. We come to the conclusion that Tsallis equilibrium distribution is corresponding to the physical state of self-gravitating system at the hydrostatic equilibrium.Comment: 11 pages, 25 references. The Twelfth United Nations/European Space Agency workshop on basic space science. May 24-28 2004, Beijing, Chin