Genistein induces a protective immunomodulatory effect in a mouse model of cervical cancer

Background: Genistein (GEN), a naturally occurring flavonoid present in soy bean, has attracted scientific interest for its possible benefits in cancer. Objective: The potential immunomodulatory effects of genistein on the immune system and against TC-1 tumor cell line were evaluated in adult female C57BL/6 mice. Methods: Mice were treated with GEN 10 days before to 10 days after the tumor induction. Thirty days after the last GEN treatment, lymphocyte proliferation, Lactase Dehydrogenase (LDH) cytolytic activity and cytokine secretion were analyzed in GEN and control groups. Results: The results showed that ingestion of genistein significantly increased lymphocyte proliferation and LDH release. Furthermore, the treatment with genistein also caused a significant increment in interferon gamma (IFN-γ). In addition, the treatment achieved significant therapeutic effect in tumor models compared to the control group. These results indicated that the effect of GEN on tumor growth may be attributed to its effect on lymphocyte proliferation, cytolytic activity and IFN-γ production. Conclusion: These results demonstrate that GEN exerts an immunomodulatory effect in a mouse model of Human Papillomavirus (HPV) associated-cervical cancer

Use of leisure time in cardiovascular patients in Gorgan (South East of Caspian Sea)

The aim of this study was to compare activity patterns and leisure time between matched groups of patients with cardiovascular disease and individuals without a heart disease. The study included 100 patients recruited from those referred to cardiology department of 5th Azar General Hospital of Golestan University of Medical Sciences in Gorgan (South East of Caspian Sea) and 100 matched control subjects during the period 2007-2008. Odds ratios (OR), together with 95% confidence intervals (95% CI), were calculated using logistic regression, as estimates of relative risks. Listening to music OR = 8.800 (95% CI: 2.717-28.499, p<0.05), meditation OR = 6.111 (95% CI; 2.616-14.274, p<0.05) were independent risk factors. Subjects who performed 2 h per week and 2-4 h per week physical activity, the odds ratios were 0.038 ( 95% CI: 0.012-0.124, p<0.05) and 0.079, (95% CI: 0.024-0.260, p<0.05), respectively. Low physical activity and use of long time relaxation are associated with cardiovascular disease in these patients. Regular participation in physical activity such as walking 2 h per week and 2-4 h per week, are associated with reduced risk of cardiovascular disease. This study suggests the importance of both leisure-time physical activity and sedentary behaviors in the prevention of CVD. © 2009 Asian Network for Scientific Information

Predicting the outcome in patients with unexplained syncope and suspected cardiac cause: Role of electrophysiologic studies Kalp nedenli oldu�undan ��phe edilen ve a�ıklanamayan senkoplu bir hastada sonucun kestirilmesi

Objective: Unexplained syncope is a challenge facing electrophysiologists. The prognosis varies widely depending on underlying causes, specially, cardiac ones. We sought to determine the abnormal electrophysiolgic (EP) study results as predictors of prognosis in syncope patients with suspected cardiac cause and risk factors associated with mortality. Methods: A total of 227 consecutive patients with unexplained syncope were prospectively enrolled in this study. EP study was performed in 177 patients in base of inclusion criteria. These patients, in whom a cardiac cause of syncope was suspected, underwent EP study and if negative, head-up tilts test (HUTT). Complete follow-up was obtained for 132 patients for 20.0�10.8 months. Results: A cardiac cause of syncope was established in 35, a neurally mediated syncope in 35.6, and in the rest 29.4 the cause of syncope remained unexplained despite a throughout neurologic and cardiologic evaluation. Logistic analysis revealed that the significant predictors of a cardiac cause of syncope were the absence of prodromal symptoms, left bundle branch block (LBBB), sever left ventricle (LV) dysfunction and male gender. At logistic analysis, the presence of LBBB (OR=6.63; 95 CI: 1.09-40) was significantly associated with outcome of death. Conclusion: The present study provides evidence that presence of LBBB, abnormal EP study result and structural heart disease (SHD) have prognostic value in patients with suspected cardiac cause of syncope. The patients with SHD and unexplained syncope who had a negative EP study have a good long-term prognosis even in the presence of LV dysfunction. � 2015 by Turkish Society of Cardiology

Predicting the outcome in patients with unexplained syncope and suspected cardiac cause: Role of electrophysiologic studies

Objective: Unexplained syncope is a challenge facing electrophysiologists. The prognosis varies widely depending on underlying causes, specially, cardiac ones. We sought to determine the abnormal electrophysiolgic (EP) study results as predictors of prognosis in syncope patients with suspected cardiac cause and risk factors associated with mortality. Methods: A total of 227 consecutive patients with unexplained syncope were prospectively enrolled in this study. EP study was performed in 177 patients in base of inclusion criteria. These patients, in whom a cardiac cause of syncope was suspected, underwent EP study and if negative, head-up tilts test (HUTT). Complete follow-up was obtained for 132 patients for 20.0±10.8 months. Results: A cardiac cause of syncope was established in 35, a neurally mediated syncope in 35.6, and in the rest 29.4 the cause of syncope remained unexplained despite a throughout neurologic and cardiologic evaluation. Logistic analysis revealed that the significant predictors of a cardiac cause of syncope were the absence of prodromal symptoms, left bundle branch block (LBBB), sever left ventricle (LV) dysfunction and male gender. At logistic analysis, the presence of LBBB (OR=6.63; 95 CI: 1.09-40) was significantly associated with outcome of death. Conclusion: The present study provides evidence that presence of LBBB, abnormal EP study result and structural heart disease (SHD) have prognostic value in patients with suspected cardiac cause of syncope. The patients with SHD and unexplained syncope who had a negative EP study have a good long-term prognosis even in the presence of LV dysfunction. © 2015 by Turkish Society of Cardiology

The effect of endurance training on the level of tissue IL-6 and VEGF in mice with breast cancer

زمینه و هدف: تمرینات ورزش پتانسیلی در جهت پیشگیری از سرطان پستان دارد. هدف پژوهش حاضر بررسی اثرات پیشگیری و کمک درمانی تمرینات ورزشی بر سایتوکاین های درگیر در رگ زایی تومور سرطان پستان وابسته به گیرنده استروژن می باشد. روش بررسی: در این مطالعه مداخله ای 50 سر موش بالب سی ماده به طور تصادفی در چهار گروه قرار گرفتند. پس از آشناسازی با محیط دو گروه از موش ها به مدت 8 هفته تمرین استقامتی تداومی را انجام دادند و سپس سلول های سرطانی وابسته به استروژن (MC4-L2) به همه موش ها تزریق گردید. پس از آن یک گروه از موش های تمرین کرده و یک گروه از موش های تمرین نکرده به مدت 6 هفته، 5 روز در هفته تمرینات استقامتی را انجام دادند. حجم تومور به صورت هفتگی با کولیس دیجیتالی اندازه گیری شد. در پایان موش ها قربانی شدند و بافت تومور برداشته و سطوح سایتوکاین های اینترلوکین 6 (IL-6) و فاکتور رشد اپی تلیال عروق (VEGF) با روش الایزا اندازه گیری شد. یافته ها: بین گروه ها در میزان مقادیر IL-6 و VEGF و میزان رشد تومور تفاوت معناداری وجود داشت (001/0PP). نتیجه گیری: با توجه به افت مقادیر IL-6 و VEGF در گروه هایی که قبل از سرطانی شدن و پس از سرطانی شدن تمرینات ورزشی را انجام دادند؛ می توان گفت که تمرینات ورزشی علاوه بر نقش پیشگیرانه بسیار موثر، دارای نقش درمانی در تومورهای وابسته به گیرنده استروژن نیز می باشند

Study of the relationship between HPA-1 and HPA-5 gene polymorphisms and refractory to platelet therapy and recombinant factor VII in glanzmann thrombasthenia patients in Southeast of Iran

Background: Glanzmann Thrombasthenia (GT) is a rare autosomal disease. HPA (Human Platelet Alloantigen) is a surface polymorphic alloantigen of platelets. This study was intended to investigate and compare the polymorphism of HPA-1 and HPA-5 genes in two groups of GT patients, with and without resistance to platelet and recombinant factor VII therapy. Materials and Methods: This case control study was performed on GT patients (n=16) with resistance to platelet therapy and recombinant factor VII and control group of GT patients (n=16) without resistance to platelet therapy and recombinant factor VII. The consent form was completed by each patient. Gene polymorphisms of HPA-1 and HPA-5 were investigated using SSP-PCR, and the obtained data were analyzed using statistical software SPSS16.0. Results: The results indicated no significant relationship between the studied genes and their resistance to platelet therapy and recombinant factor VII. The frequencies of HPA-1 genotype a/a were 98 and 94 in patient and control groups, respectively. The frequency of allele b was found to be less than allele a. The value of this allele was 4 in patient group and 1 in control group. In addition, the HPA-5a/a (98) was the most frequent alloantigen?? (check it) in both groups. Seven percent (7) of the patients had the HPA-5a/b genotype, and the HPA-5b/b was found to be absent in these individuals. Conclusion: According to the results obtained, it could be concluded that these genes play no role in resistance to platelet therapy. © 2018, Tehran University of Medical Sciences (TUMS). All rights reserved

Latent atriofascicular pathway participating in a wide complex tachycardia: Differentiation from ventricular tachycardia

Accessory pathways with anterograde decremental conduction properties usually are characterized by presence of antegrade preexcitation during atrial pacing. We report a 38-year-old man with frequent episodes of palpitation. No evidence of ventricular preexcitation was seen during sinus rhythm or atrial pacing. All electrophysiologic maneuvers were compatible with an antidromic tachycardia using atriofascicular pathway as the antegrade limb and the atrioventricular nodal pathway as retrograde limb. Radiofrequency ablation at recording site of accessory pathway potential resulted in cure of tachycardia with no recurrence during 3-month follow-up. This report indicated that atriofascicular pathway-mediated tachycardia should be considered in differential diagnosis of all cases of wide complex tachycardia with left bundle branch morphology and left axis. © 2006, The Authors

Association of P1635 and P1655 polymorphisms in dysbindin (DTNBP1) gene with schizophrenia

Alizadeh F, Tabatabaiefar MA, Ghadiri M, Yekaninejad MS, Jalilian N, Noori-Daloii MR. Association of P1635 and P1655 polymorphisms in dysbindin (DTNBP1) gene with schizophrenia. Objectives: Schizophrenia (SCZ) is a severe psychiatric disorder with a lifetime prevalence of approximately 1 in most of the populations studied. SCZ is multifactorial with the contribution of multiple susceptibility genes that could act in conjunction with epigenetic processes and environmental factors. There is some evidence supporting the association between genetic variants in dysbindin (DTNBP1) gene and SCZ in populations. In this study, we investigated the association between polymorphisms P1635 and P1655 in dysbindin gene with SCZ. Methods: Totally, 115 unrelated patients with SCZ and 117 unrelated healthy volunteers were studied. Genomic DNA was extracted from blood. Genotyping was done with the PCR-RFLP method. The allele and genotype associations were analysed with �2 test. The Benjamini-Hochberg procedure was used to correct p values for multiple comparisons. Results: The results showed no significant difference between patients and controls in allelic frequencies or genotypic distributions of SNP P1635 (p = 0.809), but a significant difference between the case and control groups for SNP P1655 (p = 0.009) was found. We could also find a significant positive association between A-C haplotype and SCZ (OR = 1.7, 95 CI 1.18-2.42; p = 0.004, pc = 0.02) and a protective effect for A-G haplotype (p = 0.003, OR = 0.57, 95 CI 1.18-2.42; p = 0.003, pc = 0.02). Conclusion: This study may provide further support for the association between SNP polymorphisms in DTNBP1 and SCZ in the Iranian population. Studies with more markers and subjects for various populations will be necessary to understand the genetic contribution of the gene to the development of SCZ. © 2011 John Wiley &amp; Sons A/S