Susceptibility of Some Bacterial Contaminants Recovered from Commercial Cosmetics in Jordan to Preservatives and Antibiotics

Purpose: To investigate the susceptibility of bacterial contaminants recovered from cosmetics to preservatives and antibiotics.Methods: Nine bacterial isolates recovered from various brands of commercially available cosmetics marketed in Jordan were tested for their susceptibility pattern against two paraben esters and two formaldehyde donors in addition to nine commonly used antibiotics. The biocidal effect for three preservatives was tested at 0.2 % concentration while the fourth was determined at a strength of 0.3 %. Antibiotic sensitivity test was carried out using standard disc diffusion method.Results: Isolates of Pseudomonas aeruginosa exhibited high resistance pattern to most of the tested preservatives and antibiotics; only one isolate was sensitive to imidazolidinyl urea while others were resistant to the 4 preservatives tested. Each of these isolates exhibited resistance to at least 5 antibiotics. Other organisms, including Escherichia coli and Staphylococcus aureus, were resistant to the class of preservatives used in the various formulations from which they were recovered and demonstrated resistance to fewer antibiotics. Coagulase-negative staphylococci were the most sensitive to both categories of antimicrobials used. One isolate was sensitive to all preservatives whereas the same isolate was resistant to only co-trimoxazole. Ciprofloxacin and norfloxacin demonstrated the highest in vitro antimicrobial effect against the contaminants investigated.Conclusion: The bacterial contaminants of cosmetics exhibited variable cross resistance between preservatives and antibiotics. This cross resistance was species- and even strain-specific.Keywords: Antibiotic, Bacterial contaminant, Cosmetics, Preservative, Resistance

Microbiological Quality of Blister Pack Tablets in Community Pharmacies in Jordan

Purpose: To investigate the microbiological quality of blister-packed tablets manufactured and marketed in Jordan in order to assess Good Manufacturing Practice by pharmaceutical industries in the country.Methods: A total of 66 items of 22 registered blister-packed tablet brands were purchased from community pharmacies in Amman. All the items were investigated for total bacterial count and the presence of specified microorganisms using compendial procedures.Results: Out of 66 items purchased, forty eight (72.7 %) products were free from microbial contamination, while 11 (16.7 %) harbored bacteria in counts < 102 cfu/g. The remaining 7 (10.6 %) items contained counts between 102 and < 103 cfu/g. The most commonly encountered contaminant was Bacillus species but specified objectionable bacteria such as Escherichia coli and Staphylococcus aereus were not detected. Aspergillus and Penicillium species were isolated in low numbers from few products. Six of the products with the highest stratified bacterial count were manufactured by one company and were also found to be contaminated with Aeromonas species.Conclusion: This study demonstrates that blister-packed tablets produced by Jordanian pharmaceutical companies are within acceptable microbiological quality but the detection of microbial load in some brands close to the maximum allowable limit suggests the need for better adherence to GMP in the country.Keywords: Blister pack, Community pharmacy, Good Manufacturing Practice, Microbial contamination,Quality control, Tablet

Enhanced Position Verification for VANETs using Subjective Logic

The integrity of messages in vehicular ad-hoc networks has been extensively studied by the research community, resulting in the IEEE~1609.2 standard, which provides typical integrity guarantees. However, the correctness of message contents is still one of the main challenges of applying dependable and secure vehicular ad-hoc networks. One important use case is the validity of position information contained in messages: position verification mechanisms have been proposed in the literature to provide this functionality. A more general approach to validate such information is by applying misbehavior detection mechanisms. In this paper, we consider misbehavior detection by enhancing two position verification mechanisms and fusing their results in a generalized framework using subjective logic. We conduct extensive simulations using VEINS to study the impact of traffic density, as well as several types of attackers and fractions of attackers on our mechanisms. The obtained results show the proposed framework can validate position information as effectively as existing approaches in the literature, without tailoring the framework specifically for this use case.Comment: 7 pages, 18 figures, corrected version of a paper submitted to 2016 IEEE 84th Vehicular Technology Conference (VTC2016-Fall): revised the way an opinion is created with eART, and re-did the experiments (uploaded here as correction in agreement with TPC Chairs

Effect of ethnomedicinal plants used in folklore medicine in Jordan as antibiotic resistant inhibitors on Escherichia coli

<p>Abstract</p> <p>Background</p> <p><it>Escherichia coli </it>occurs naturally in the human gut; however, certain strains that can cause infections, are becoming resistant to antibiotics. Multidrug-resistant <it>E. coli </it>that produce extended-spectrum β lactamases (ESBLs), such as the CTX-M enzymes, have emerged within the community setting as an important cause of urinary tract infections (UTIs) and bloodstream infections may be associated with these community-onsets. This is the first report testing the antibiotic resistance-modifying activity of nineteen Jordanian plants against multidrug-resistant <it>E. coli</it>.</p> <p>Methods</p> <p>The susceptibility of bacterial isolates to antibiotics was tested by determining their minimum inhibitory concentrations (MICs) using a broth microdilution method. Nineteen Jordanian plant extracts (<it>Capparis spinosa </it>L., <it>Artemisia herba-alba Asso, Echinops polyceras </it>Boiss., <it>Gundelia tournefortii </it>L, <it>Varthemia iphionoides </it>Boiss. & Blanche, <it>Eruca sativa Mill</it>., <it>Euphorbia macroclada </it>L., <it>Hypericum trequetrifolium </it>Turra, <it>Achillea santolina </it>L., <it>Mentha longifolia </it>Host, <it>Origanum syriacum </it>L., <it>Phlomis brachydo</it>(Boiss.) Zohary, <it>Teucrium polium </it>L., <it>Anagyris foetida </it>L., <it>Trigonella foenum-graecum </it>L., <it>Thea sinensis </it>L., <it>Hibiscus sabdariffa </it>L., <it>Lepidium sativum </it>L., <it>Pimpinella anisum </it>L.) were combined with antibiotics, from different classes, and the inhibitory effect of the combinations was estimated.</p> <p>Results</p> <p>Methanolic extracts of the plant materials enhanced the inhibitory effects of chloramphenicol, neomycin, doxycycline, cephalexin and nalidixic acid against both the standard strain and to a lesser extent the resistant strain of <it>E. coli</it>. Two edible plant extracts (<it>Gundelia tournefortii L</it>. and <it>Pimpinella anisum L</it>.) generally enhanced activity against resistant strain. Some of the plant extracts like <it>Origanum syriacum </it>L.(Labiateae), <it>Trigonella foenum- graecum </it>L.(Leguminosae), <it>Euphorbia macroclada </it>(Euphorbiaceae) and <it>Hibiscus sabdariffa </it>(Malvaceae) did not enhance the activity of amoxicillin against both standard and resistant <it>E. coli</it>. On the other hand combinations of amoxicillin with other plant extracts used showed variable effect between standard and resistant strains. Plant extracts like <it>Anagyris foetida </it>(Leguminosae) and <it>Lepidium sativum </it>(Umbelliferae) reduced the activity of amoxicillin against the standard strain but enhanced the activity against resistant strains. Three edible plants; Gundelia <it>tournefortii </it>L. (Compositae) <it>Eruca sativa </it>Mill. (Cruciferae), and <it>Origanum syriacum </it>L. (Labiateae), enhanced activity of clarithromycin against the resistant <it>E. coli </it>strain.</p> <p>Conclusion</p> <p>This study probably suggests possibility of concurrent use of these antibiotics and plant extracts in treating infections caused by <it>E. coli </it>or at least the concomitant administration may not impair the antimicrobial activity of these antibiotics.</p

Kinetics of biodegradation of diethylketone by Arthrobacter viscosus

The performance of an Arthrobacter viscosus culture to remove diethylketone from aqueous solutions was evaluated. The effect of initial concentration of diethylketone on the growth of the bacteria was evaluated for the range of concentration between 0 and 4.8 g/l, aiming to evaluate a possible toxicological effect. The maximum specific growth rate achieved is 0.221 h-1 at 1.6 g/l of initial diethylketone concentration, suggesting that for higher concentrations an inhibitory effect on the growth occurs. The removal percentages obtained were approximately 88%, for all the initial concentrations tested. The kinetic parameters were estimated using four growth kinetic models for biodegradation of organic compounds available in the literature. The experimental data found is well fitted by the Haldane model (R2 = 1) as compared to Monod model (R2 = 0.99), Powell (R2 = 0.82) and Loung model (R2 = 0.95). The biodegradation of diethylketone using concentrated biomass was studied for an initial diethylketone concentration ranging from 0.8–3.9 g/l in a batch with recirculation mode of operation. The biodegradation rate found followed the pseudo-second order kinetics and the resulting kinetic parameters are reported. The removal percentages obtained were approximately 100%, for all the initial concentrations tested, suggesting that the increment on the biomass concentration allows better results in terms of removal of diethylketone. This study showed that these bacteria are very effective for the removal of diethylketone from aqueous solutions.The authors would like to gratefully acknowledge the financial support of this project by the Fundacao para a Ciencia e Tecnologia (FCT), Ministerio da Ciencia e Tecnologia, Portugal and Fundo Social Europeu (FSE). Cristina Quintelas thanks FCT for a Post-Doc grant

Muslim active citizenship in Australia: Socioeconomic challenges and the emergence of a Muslim elite

The most recent national Census demonstrated that Australian Muslims continue to occupy a socioeconomically disadvantaged position. On key indicators of unemployment rate, income, type of occupation and home ownership, Muslims consistently under-perform the national average. This pattern is evident in the last three Census data (2001, 2006 and 2011). Limited access to resources and a sense of marginalisation challenge full engagement with society and the natural growth of emotional affiliation with Australia. Muslim active citizenship is hampered by socioeconomic barriers. At the same time, an increasingly proactive class of educated Muslim elite has emerged to claim a voice for Muslims in Australia and promote citizenship rights and responsibilities. <br /

HESEB The Helmholtz state of the art Soft X Ray Undulator beamline at SESAME

SESAME and a consortium of five Helmholtz Centers are designing and installing a state of the art soft X Ray undulator beamline at the SESAME light source in Amman, Jordan. Funding is provided by the Helmholtz Association over a four year project cycle that started in January 2019. This is an interim report covering the first 36 months of the project where the construction and installation has been almost completed and commissioning and characterization of the beamline is about to start. Additionally, seminars, workshops, and a training program are part of the project aimed at establishing a broad user communit