Comparison of surface chlorophyll, primary production, and satellite imagery in hydrographically different sounds off southern New England

Block Island Sound (BIS) and Rhode Island Sound (RIS) are adjacent inner continental shelf ecosystems with contrasting hydrographic regimes. BIS exhibits more energetic tidal mixing, and water column stratification remains weak but persists year-round due to nearby estuarine exchange flow; RIS is less influenced by estuaries, and more seasonal with strong stratification in summer. We compared annual cycles of phytoplankton biomass and primary production in BIS and RIS using measurements (surface chlorophyll, 14C primary production), primary production models (Webb/Platt and BZE models), and satellite ocean color products. During 22 mo of sampling, measured surface chlorophyll was not significantly different between BIS (mean = 1.86 mg m-3) and RIS (1.69 mg m-3), and bimodal peaks of phytoplankton biomass and production occurred concurrently in both Sounds. In contrast, a 12 yr ocean-color based chlorophyll time series indicated higher long-term average surface chlorophyll in the more well-mixed system (BIS, mean = 1.50 mg m-3; RIS, mean = 0.86 mg m-3). BIS annual primary production (318 to 329 g C m-2 yr-1) was higher than RIS (239 to 256 g C m-2 yr-1; p \u3c 0.001). These differences were most apparent during the summer, concurrent with the largest differences in water column stratification. Phytoplankton bloom phenology was driven by physical processes, with chlorophyll significantly related to water column stratification (r = -0.51, p = 0.01), depth of the euphotic zone (r = -0.54, p = 0.05), and surface water salinity (r = 0.54, p = 0.04). Primary production was correlated with surface water temperature (r = 0.57, p = 0.03) but the mechanisms underlying production differences between the Sounds remain unresolved. We hypothesize that different hydrographies give rise to different productivity between the Sounds

Generation of <em>Escherichia coli</em> nitroreductase mutants conferring improved cell sensitization to the prodrug CB1954

Escherichia coli nitroreductase (NTR) activates the prodrug CB1954 to a cytotoxic derivative, allowing selective sensitization of NTR-expressing cells or tumors to the prodrug. This is one of several enzyme-prodrug combinations that are under development for cancer gene therapy, and the system has now entered clinical trials. Enhancing the catalytic efficiency of NTR for CB1954 could improve its therapeutic potential. From the crystal structure of an enzyme-ligand complex, we identified nine amino acid residues within the active site that could directly influence prodrug binding and catalysis. Mutant libraries were generated for each of these residues and clones screened for their ability to sensitize E. coli to CB1954. Amino acid substitutions at six positions conferred markedly greater sensitivity to CB1954 than did the WT enzyme; the best mutants, at residue F124, resulted in ∼5-fold improvement. Using an adenovirus vector, we introduced the F124K NTR mutant into human SK-OV-3 ovarian carcinoma cells and showed it to be ∼5-fold more potent in sensitizing the cells to CB1954 at the clinically relevant prodrug concentration of 1 μM than was the WT enzyme. Enhanced mutant NTRs such as F124K should improve the efficacy of the NTR/CB1954 combination in cancer gene therapy

A communication platform demonstrator for new generation railway traffic management systems: Testing and validation

\ua9 2023 The Author(s). Current rail traffic management and control systems cannot be easily upgraded to the new needs and challenges of modern railway systems because they do not offer interoperable data structures and standardized communication interfaces. To meet this need, the Horizon 2020 Shift2Rail OPTIMA project has developed a communication platform for testing and validating the new generation of traffic management systems (TMS), whose main innovative features are the interoperability of the data structures used, standardization of communications, continuous access to real-time and persistent data from heterogeneous data sources, modularity of components and scalability of the platform. This paper presents the main components, their functions and characteristics, then describes the testing and validation of the platform, even when federated with other innovative TMS modules developed in separate projects. The successful validation of the system has confirmed the achievement of the objectives set and allowed a new set of objectives to be defined for the reference platform for the railway TMS/Traffic Control systems

Role of human milk oligosaccharides in Group B Streptococcus colonisation.

Group B Streptococcus (GBS) infection is a major cause of morbidity and mortality in infants. The major risk factor for GBS disease is maternal and subsequent infant colonisation. It is unknown whether human milk oligosaccharides (HMOs) protect against GBS colonisation. HMO production is genetically determined and linked to the Lewis antigen system. We aimed to investigate the association between HMOs and infant GBS colonisation between birth and postnatal day 90. Rectovaginal swabs were collected at delivery, as well as colostrum/breast milk, infant nasopharyngeal and rectal swabs at birth, 6 days and days 60-89 postpartum from 183 Gambian mother/infant pairs. GBS colonisation and serotypes were determined using culture and PCR. (1)H nuclear magnetic resonance spectroscopy was used to characterise the mother's Lewis status and HMO profile in breast milk. Mothers who were Lewis-positive were significantly less likely to be colonised by GBS (X (2)=12.50, P<0.001). Infants of Lewis-positive mothers were less likely GBS colonised at birth (X (2)=4.88 P=0.03) and more likely to clear colonisation between birth and days 60-89 than infants born to Lewis-negative women (P=0.05). There was no association between Secretor status and GBS colonisation. In vitro work revealed that lacto-N-difucohexaose I (LNDFHI) correlated with a reduction in the growth of GBS. Our results suggest that HMO such as LNDFHI may be a useful adjunct in reducing maternal and infant colonisation and hence invasive GBS disease. Secretor status offers utility as a stratification variable in GBS clinical trials

The Cross-Section Measurement for the \u3csup\u3e3\u3c/sup\u3eH (e, e\u27, K\u3csup\u3e+\u3c/sup\u3e) nnΛ Reaction

The small binding energy of the hypertriton leads to predictions of the non-existence of bound hypernuclei for isotriplet three-body systems such as nnΛ. However, invariant mass spectroscopy at GSI has reported events that may be interpreted as the bound nnΛ state. The nnΛ state was sought by missing-mass spectroscopy via the (e, e′K+) reaction at Jefferson Lab’s experimental Hall A. The present experiment has higher sensitivity to the nnΛ-state investigation in terms of better precision by a factor of about three. The analysis shown in this article focuses on the derivation of the reaction cross-section for the 3H(γ*, K+)X reaction. Events that were detected in an acceptance, where a Monte Carlo simulation could reproduce the data well (⁠ƍp/p⎮ \u3c 4%), were analyzed to minimize the systematic uncertainty. No significant structures were observed with the acceptance cuts, and the upper limits of the production cross-section of the nnΛ state were obtained to be 21 and 31nbsr-1 at the 90% confidence level when theoretical predictions of (−BΛ, Γ) = (0.25, 0.8) MeV and (0.55, 4.7) MeV, respectively, were assumed. The cross-section result provides valuable information for examining the existence of nnΛ

Should outbreak response immunization be recommended for measles outbreaks in middle- and low-income countries? An update.

Measles caused mortality in >164,000 children in 2008, with most deaths occurring during outbreaks. Nonetheless, the impact and desirability of conducting measles outbreak response immunization (ORI) in middle- and low-income countries has been controversial. World Health Organization guidelines published in 1999 recommended against ORI in such settings, although recently these guidelines have been reversed for countries with measles mortality reduction goals

Spectroscopic Study of a Possible Λ Resonance and a Pair of (e, e\u27K⁺) Reaction With a Tritium Target

A mass spectroscopy experiment with a pair of nearly identical high-resolution spectrometers and a tritium target was performed in Hall A at Jefferson Lab. Utilizing the (e,e′K+) reaction, enhancements, which may correspond to a possible Λnn resonance and a pair of ΣNN states, were observed with an energy resolution of about 1.21 MeV (σ), although greater statistics are needed to make definitive identifications. An experimentally measured Λnn state may provide a unique constraint in determining the Λn interaction, for which no scattering data exist. In addition, although bound A = 3 and 4 Σ hypernuclei have been predicted, only an A=4 Σ hypernucleus (4ΣHe) was found, utilizing the (K−,π−) reaction on a 4He target. The possible bound ΣNN state is likely a Σ⁰nn state, although this has to be confirmed by future experiments

Beam-Spin Asymmetries From Semi-Inclusive Pion Electroproduction

We have measured the moment Asin ɸLU corresponding to the polarized electron beam-spin asymmetry in semi-inclusive deep inelastic scattering. Asin ɸLU is a twist-3 quantity providing information about quark-gluon correlations. Data were taken with the CLAS Spectrometer at Jefferson Lab using a 5.498 GeV longitudinally polarized electron beam and an unpolarized liquid hydrogen target. All three pion channels (π+,π0 and π-) were measured simultaneously over a large range of kinematics within the virtuality range Q2 ≈ 1.0- 4.5 GeV2. The observable was measured with better than 1% statistical precision over a large range of z, PT, xB, and Q2, which permits comparison with several reaction models. The discussed measurements provide an upgrade in statistics over previous measurements, and serve as the first evidence for the negative sign of the π- sin ɸ moment

Novel approach for validation of innovative modules for railway traffic management systems in a virtual environment

To increase operational efficiency, resilience and capacity of the railway system, the development of modern railway traffic management system (TMS) has attracted more and more attention in recent years. To support the development and implementation of the next generation of TMS and related applications, advanced data collection, transmission and processing approaches, digitalised databases, and virtual validation platforms, etc., are required. In the context of the TMS development (addressed by Technology Demonstrator 2.9 of Shift2Rail Innovation Programme 2), this support is to be provided by a scalable, interoperable and standardised communication platform for internal and external communication between different subsystems, applications and clients. This paper outlines the approach of the ongoing OPTIMA project aimed to develop a communication platform demonstrator for railway TMS based on a novel Integration Layer (IL) and its various interfaces to entities including integration layer services, TMS service, rail business service, external services and operator workstations. Further detailed discussion in this paper relates to the approach to validating the communication platform demonstrator as a functional entity, and as a virtual testing environment to validate railway traffic management and other applications. The validation approach for the applications tested on the communication platform demonstrator is also presented. The results of future implementation of this validation approach will be used to assess the functionality of the communications platform demonstrator developed, and the initial TMS applications tested on it, and form an important step towards developing and implementing IL based communications platforms for future TMSs

Coherent Photoproduction of \u3ci\u3eπ\u3c/i\u3e\u3csup\u3e+\u3c/sup\u3e From \u3csup\u3e3\u3c/sup\u3eHe

We have measured the differential cross section for the 3He → π+t reaction. This reaction was studied using the Continuous Electron Beam Accelerator Facility (CEBAF) Large Acceptance Spectrometer (CLAS) at Jefferson Lab. Real photons produced with the Hall-B bremsstrahlung tagging system in the energy range from 0.50 to 1.55 GeV were incident on a cryogenic liquid 3He target. The differential cross sections for the 3He → π+t reaction were measured as a function of photon-beam energy and pion-scattering angle. Theoretical predictions to date cannot explain the large cross sections except at backward angles, showing that additional components must be added to the model