Spectral characterizations and antibacterial effect of 2-(5-R-1H-benzimidazol-2-YL)-4-methyl/bromo-phenols and some metal complexes

2-(5-H/Cl/Me/NO2-1H-benzimidazol-2-yl)-4-Me/Br-phenols (HL1–HL5) were synthesized. HL1 complexes with Cu(NO3)2, AgNO3, Zn(ClO4)2 and; HL4, HL5 complexes with Zn(ClO4)2 were prepared. The structures of the compounds were confirmed on the basis of elemental analysis, molar conductivity, magnetic moment, FT-IR, 1H- and 13C-NMR. Antibacterial activity of the ligands and the complexes were evaluated using the disk diffusion method in dimethyl sulfoxide (DMSO) as well as the minimal inhibitory concentration (MIC) dilution method, against nine bacteria, and the results were compared with penicillin–G and oxytetracycline. While HL1 ligand has considerable antibacterial activity on B. cereus only; it’s Ag(I) complex show antibacterial effect toward almost all the bacteria. It is highly interesting that HL5 and [Zn(HL5)(L5)]ClO4 exhibit considerable high antibacterial activity toward K. pneumoniae, B. cereus, S. epidermidis and B. subtilis. KEY WORDS: Benzimidazole, Phenol, Metal complexes, Antibacterial activity  Bull. Chem. Soc. Ethiop. 2010, 24(3), 391-400

Proširenost virusa virusnog proljeva u tkivima spolnog sustava goveda.

In this study, the cellular localisation and distribution of BVD viral antigens in genital system tissues were investigated in 65 non-pregnant dairy cows and in 65 bulls. For this study genital system tissue samples, taken from slaughtered bulls with no pathological lesions and from females having reproductive problems, were marked using the immunoperoxidase method on their paraffin sections. BVD viral antigens were determined in 15 of the 65 non-pregnant dairy cows (consistent with cell culture results) using the indirect immunoperoxidase method. BVD viral antigens were present in macrophage-like cells in the stroma of the ovaries and uterus. No BVD viral antigens were observed in the samples of testicles, epididymis, vesicula seminalis, or prostate in the male animals. In all animals, including BVDV-positive ones, no pathologic lesions were observed, except periodically non-specific subepithelial or stromal mononuclear cell infiltrations. Most of these cells were seen to be formed by lymphocytes and macrophages.Istražena je lokalizacija i proširenost antigena virusa virusnog proljeva goveda u tkivima spolnog sustava 65 negravidnih mliječnih krava i 65 bikova. U tu svrhu rabljen je imunoperoksidazni test na histološkim rezovima tkiva. Tom metodom pretraženi su uzorci tkiva spolnih organa uzeti od zdravih zaklanih bikova te krava s reprodukcijskim poremećajima. Virusni antigeni dokazani su u 15 od 65 krava (sukladno s uzgojem virusa na staničnoj kulturi). Antigeni su bili prisutni u makrofagima sličnim stanicama unutar strome jajnika i maternice. Virusni antigeni nisu dokazani u uzorcima tkiva testisa, epididimisa, sjemene vrećice i prostate bikova. U pretraženih životinja, uključujući i one pozitivne na virus, nisu zabilježene patološke promjene, osim povremenih nespecifičnih subepitelijalnih ili stromalnih mononuklearnih infiltracija limfocitima i makrofagima

Preparation, characterization and antibacterial effect of 2-methoxy-6-(5-H/Me/Cl/NO2-1H-benzimidazol-2-yl)phenols and some transition metal complexes

2-Methoxy-6-(5-H/methyl/chloro/nitro-1H-benzimidazol-2-yl)phenols (HLx; x = 1–4, respectively) ligands and HL1 complexes with Fe(NO3)3, Cu(NO3)2, AgNO3 and Zn(NO3)2 were synthesized and characterized. The structures of the compounds were confirmed based on elemental analysis, molar conductivity, magnetic moment, FT-IR, 1H- and 13C-NMR. The antibacterial activity and minimum inhibitory concentration (MIC) of the free ligands, their hydrochloride salts and the complexes were evaluated using the disk diffusion method in dimethyl sulfoxide (DMSO) and the dilution method, respectively, against 9 bacteria. HL1 and HL3, as well as the Cu(II) and Zn(II) complexes, showed antibacterial activity against Gram-positive bacteria

15-yıllık periyotta İstanbul Türkiye’de dermatofitoz şüpheli köpek ve kedilerden izole edilen dermatofitler: Güncellenmiş rapor

The present research was aimed to determine the prevalence of dermatophytes isolated from symptomatic dogs and cats, within a 15-year-period, in the city of Istanbul, Turkey. Dermatological specimens were collected from 1504 dogs and 846 cats, which were presented clinical signs of ringworm. Direct microscopy and mycological cultures were performed. The fungal growth rate was detected at 8.2% and 22.8% from dogs and cats, respectively. Microsporum canis was the most frequently isolated species followed by Trichophyton spp., M. gypseum, T. mentagrophytes, M. nanum, other Microsporum spp. moreover T. tonsurans. The cats less than two-year age and more than ten-year age showed a statistically significant higher isolation rate of infection (p < 0.05). There were no statistically significant differences between the age of the dogs and the dermatophyte isolation rate and between the gender of the dogs and cats and the dermatophyte isolation rate. As a conclusion, the data suggest an updated report on local epidemiology and define potential etiologic agentsBu araştırma, İstanbul ilinde 15 yıllık bir süre içinde semptomatik köpek ve kedilerden izole edilen dermatofitlerin yaygınlığını belirlemeyi amaçlamıştır. Dermatolojik örnekler ringworm klinik belirtileri gösteren 1504 köpek ve 846 kediden toplandı. Direkt mikroskopi ve mikolojik kültürler yapıldı. Mantar üreme oranları, köpeklerde % 8.2 kedilerde % 22.8 olarak saptandı. En sık izole edilen tür Microsporum canis idi. Bunu Trichophyton spp., M. gypseum, T. mentagrophytes, M. nanum, diğer Microsporum spp. ve T. tonsurans takip etti. İki yaşından küçük ve on yaşından büyük kediler, istatistiksel olarak anlamlı derecede yüksek bir etken izolasyon oranı gösterdi (p <0.05). Köpeklerin yaşı ve dermatofit izolasyon oranları ile kedi ve köpeklerin cinsiyeti ve dermatofit izolasyon oranları arasında istatistiksel olarak anlamlı bir fark bulunmadı. Sonuç olarak, veriler yerel epidemiyoloji üzerine güncel bir rapor sunmakta ve olası etiyolojik ajanları tanımlamaktadır

Ornithobacteriurn rhinotrachelae (OR) infection

Ornithobacterium rhinotracheale infection is characterized by respiratory disease, decreased growth and increased mortality in avians. Although a reasonably new infection, it has been found in many countries in a short period. In this article, this infection, which causes large financial losses in avian enterprises, was investigated