141 research outputs found
Role of domain walls in the abnormal photovoltaic effect in BiFeO3
Recently, the anomalous photovoltaic (PV) effect in BiFeO3 (BFO) thin
films, which resulted in open circuit voltages (V-oc) considerably
larger than the band gap of the material, has generated a revival of the
entire field of photoferroelectrics. Here, via temperature-dependent PV
studies, we prove that the bulk photovoltaic (BPV) effect, which has
been studied in the past for many non-centrosymmetric materials, is at
the origin of the anomalous PV effect in BFO films. Moreover, we show
that irrespective of the measurement geometry, V-oc as high as 50V can
be achieved by controlling the conductivity of domain walls (DW). We
also show that photoconductivity of the DW is markedly higher than in
the bulk of BFO
Electrically controlled long-distance spin transport through an antiferromagnetic insulator
Spintronics uses spins, the intrinsic angular momentum of electrons, as an
alternative for the electron charge. Its long-term goal is in the development
of beyond-Moore low dissipation technology devices. Recent progress
demonstrated the long-distance transport of spin signals across ferromagnetic
insulators. Antiferromagnetically ordered materials are however the most common
class of magnetic materials with several crucial advantages over ferromagnetic
systems. In contrast to the latter, antiferromagnets exhibit no net magnetic
moment, which renders them stable and impervious to external fields. In
addition, they can be operated at THz frequencies. While fundamentally their
properties bode well for spin transport, previous indirect observations
indicate that spin transmission through antiferromagnets is limited to short
distances of a few nanometers. Here we demonstrate the long-distance, over tens
of micrometers, propagation of spin currents through hematite (\alpha-Fe2O3),
the most common antiferromagnetic iron oxide, exploiting the spin Hall effect
for spin injection. We control the spin current flow by the interfacial
spin-bias and by tuning the antiferromagnetic resonance frequency with an
external magnetic field. This simple antiferromagnetic insulator is shown to
convey spin information parallel to the compensated moment (N\'eel order) over
distances exceeding tens of micrometers. This newly-discovered mechanism
transports spin as efficiently as the net magnetic moments in the best-suited
complex ferromagnets. Our results pave the way to ultra-fast, low-power
antiferromagnet-insulator-based spin-logic devices that operate at room
temperature and in the absence of magnetic fields
Complete In Vitro Life Cycle of Trypanosoma congolense: Development of Genetic Tools
Trypanosoma congolense is a parasite responsible for severe disease of African livestock. Its life cycle is complex and divided into two phases, one in the tsetse fly vector and one in the bloodstream of the mammalian host. Molecular tools for gene function analyses in parasitic organisms are essential. Previous studies described the possibility of completing the entire T. congolense life cycle in vitro. However, the model showed major flaws including the absence of stable long-term culture of the infectious bloodstream forms, a laborious time-consuming period to perform the cycle and a lack of genetic tools. We therefore aimed to develop a standardized model convenient for genetic engineering. We succeeded in producing long-term cultures of all the developmental stages on long-term, to define all the differentiation steps and to finally complete the whole cycle in vitro. This improved model offers the opportunity to conduct phenotype analyses of genetically modified strains throughout the in vitro cycle and also during experimental infections
AKAP95 regulates splicing through scaffolding RNAs and RNA processing factors
YesAlternative splicing of pre-mRNAs significantly contributes to the complexity of gene
expression in higher organisms, but the regulation of the splice site selection remains
incompletely understood. We have previously demonstrated that a chromatin-associated
protein, AKAP95 (AKAP8), has a remarkable activity in enhancing chromatin transcription.
In this study, we have shown that AKAP95 physically interacts with many factors involved in
transcription and RNA processing, and functionally regulates pre-mRNA splicing. AKAP95
directly promotes splicing in vitro and the inclusion of a specific exon of an endogenous gene
FAM126A. The N-terminal YG-rich domain of AKAP95 is important for its binding to RNA
processing factors including selective groups of hnRNP proteins, and its zinc finger domains
are critical for pre-mRNA binding. Genome-wide binding assays revealed that AKAP95 bound
preferentially to proximal intronic regions on a large number of pre-mRNAs in human
transcriptome, and AKAP95 depletion predominantly resulted in reduced inclusion of many
exons. AKAP95 also selectively coordinates with hnRNP H/F and U proteins in regulating
alternative splicing events. We have further shown that AKAP95 directly interacts with itself.
Taken together, our results establish AKAP95 as a novel and mostly positive regulator of premRNA
splicing and a possible integrator of transcription and splicing regulation, and support
a model that AKAP95 facilitates the splice site communication by looping out introns through
both RNA-binding and protein-protein interaction.This work was supported by a UAB start-up fund to H.J
Promoter-bound METTL3 maintains myeloid leukaemia by m6A-dependent translation control.
N6-methyladenosine (m6A) is an abundant internal RNA modification in both coding and non-coding RNAs that is catalysed by the METTL3-METTL14 methyltransferase complex. However, the specific role of these enzymes in cancer is still largely unknown. Here we define a pathway that is specific for METTL3 and is implicated in the maintenance of a leukaemic state. We identify METTL3 as an essential gene for growth of acute myeloid leukaemia cells in two distinct genetic screens. Downregulation of METTL3 results in cell cycle arrest, differentiation of leukaemic cells and failure to establish leukaemia in immunodeficient mice. We show that METTL3, independently of METTL14, associates with chromatin and localizes to the transcriptional start sites of active genes. The vast majority of these genes have the CAATT-box binding protein CEBPZ present at the transcriptional start site, and this is required for recruitment of METTL3 to chromatin. Promoter-bound METTL3 induces m6A modification within the coding region of the associated mRNA transcript, and enhances its translation by relieving ribosome stalling. We show that genes regulated by METTL3 in this way are necessary for acute myeloid leukaemia. Together, these data define METTL3 as a regulator of a chromatin-based pathway that is necessary for maintenance of the leukaemic state and identify this enzyme as a potential therapeutic target for acute myeloid leukaemia
Direct stau production at hadron colliders in cosmologically motivated scenarios
We calculate dominant cross section contributions for stau pair production at
hadron colliders within the MSSM, taking into account left-right mixing of the
stau eigenstates. We find that b-quark annihilation and gluon fusion can
enhance the cross sections by more than one order of magnitude with respect to
the Drell-Yan predictions. These additional production channels are not yet
included in the common Monte Carlo analysis programs and have been neglected in
experimental analyses so far. For long-lived staus, we investigate differential
distributions and prospects for their stopping in the collider detectors. New
possible strategies are outlined to determine the mass and width of the heavy
CP-even Higgs boson H0. Scans of the relevant regions in the CMSSM are
performed and predictions are given for the current experiments at the LHC and
the Tevatron. The obtained insights allow us to propose collider tests of
cosmologically motivated scenarios with long-lived staus that have an
exceptionally small thermal relic abundance.Comment: 49 pages, 13 figures; v2: references added, typos corrected, text
streamlined, results unchange
- …