23 research outputs found
Characterization of Chromosomal Instability in Murine Colitis-Associated Colorectal Cancer
Patients suffering from ulcerative colitis (UC) bear an increased risk for colorectal cancer. Due to the sparsity of colitis-associated cancer (CAC) and the long duration between UC initiation and overt carcinoma, elucidating mechanisms of inflammation-associated carcinogenesis in the gut is particularly challenging. Adequate murine models are thus highly desirable. For human CACs a high frequency of chromosomal instability (CIN) reflected by aneuploidy could be shown, exceeding that of sporadic carcinomas. The aim of this study was to analyze mouse models of CAC with regard to CIN. Additionally, protein expression of p53, beta-catenin and Ki67 was measured to further characterize murine tumor development in comparison to UC-associated carcinogenesis in men.The AOM/DSS model (nâ=â23) and IL-10(-/-) mice (nâ=â8) were applied to monitor malignancy development via endoscopy and to analyze premalignant and malignant stages of CACs. CIN was assessed using DNA-image cytometry. Protein expression of p53, beta-catenin and Ki67 was evaluated by immunohistochemistry. The degree of inflammation was analyzed by histology and paralleled to local interferon-Îł release.CIN was detected in 81.25% of all murine CACs induced by AOM/DSS, while all carcinomas that arose in IL-10(-/-) mice were chromosomally stable. Beta-catenin expression was strongly membranous in IL-10(-/-) mice, while 87.50% of AOM/DSS-induced tumors showed cytoplasmatic and/or nuclear translocation of beta-catenin. p53 expression was high in both models and Ki67 staining revealed higher proliferation of IL-10(-/-)-induced CACs.AOM/DSS-colitis, but not IL-10(-/-) mice, could provide a powerful murine model to mechanistically investigate CIN in colitis-associated carcinogenesis
How to combat cyanobacterial blooms: strategy toward preventive lake restoration and reactive control measures
Effect of fingerprints orientation on skin vibrations during tactile exploration of textured surfaces
Diversidade e métodos de amostragem de Hymenoptera na cultura da melancia no semiårido
Resumo: Os objetivos deste trabalho foram conhecer a fauna de Hymenoptera associada Ă cultura da melancia e avaliar a influĂȘncia das armadilhas Pitfall, Moericke e McPhail na captura desses insetos, em ambiente semiĂĄrido no estado do Rio Grande do Norte. O levantamento foi realizado entre os meses de agosto e setembro de 2011, em ĂĄrea de produção comercial de melancia cv. Crimson Sweet. As coletas dos himenĂłpteros foram realizadas, semanalmente, durante o ciclo da cultura. Para captura dos insetos, foram utilizados trĂȘs tipos de armadilhas, Pitfall, Moericke e McPhail, na densidade de 20, 20 e 1 armadilha por hectare, respectivamente. As armadilhas foram instaladas sete dias apĂłs o plantio das sementes, e mantidas na ĂĄrea atĂ© a colheita dos frutos. Foram coletados um total de 3.123 himenĂłpteros pertencentes a 10 superfamĂlias, distribuĂdas em 24 famĂlias. Formicidae foi a mais representativa, com abundĂąncia relativa total de 54,43%, seguida por Apidae com 17,96%. Foi observada tambĂ©m a presença de 18 famĂlias de himenĂłpteros parasitoides (18,89%), com destaque para Platygastridae (6,60%), Encyrtidae (2,79%), Chalcididae (2,56%), Mymaridae (2,56%), Pompilidae (1,15%) e Trichogrammatidae (1,09%). Ressalta-se ainda a ocorrĂȘncia das famĂlias de predadores Crabronidae (6,34%), Vespidae (2,24%) e Sphecidae (0,10%). Dentre as armadilhas, Moericke capturou a maior diversidade de Hymenoptera (24 famĂlias), seguida por Pitfall (11 famĂlias) e McPhail (sete famĂlias)