Rubisco evolution in C₄ eudicots: an analysis of Amaranthaceae sensu lato.

BACKGROUND: Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyses the key reaction in the photosynthetic assimilation of CO₂. In C₄ plants CO₂ is supplied to Rubisco by an auxiliary CO₂-concentrating pathway that helps to maximize the carboxylase activity of the enzyme while suppressing its oxygenase activity. As a consequence, C₄ Rubisco exhibits a higher maximum velocity but lower substrate specificity compared with the C₃ enzyme. Specific amino-acids in Rubisco are associated with C₄ photosynthesis in monocots, but it is not known whether selection has acted on Rubisco in a similar way in eudicots. METHODOLOGY/PRINCIPAL FINDINGS: We investigated Rubisco evolution in Amaranthaceae sensu lato (including Chenopodiaceae), the third-largest family of C₄ plants, using phylogeny-based maximum likelihood and Bayesian methods to detect Darwinian selection on the chloroplast rbcL gene in a sample of 179 species. Two Rubisco residues, 281 and 309, were found to be under positive selection in C₄ Amaranthaceae with multiple parallel replacements of alanine by serine at position 281 and methionine by isoleucine at position 309. Remarkably, both amino-acids have been detected in other C₄ plant groups, such as C₄ monocots, illustrating a striking parallelism in molecular evolution. CONCLUSIONS/SIGNIFICANCE: Our findings illustrate how simple genetic changes can contribute to the evolution of photosynthesis and strengthen the hypothesis that parallel amino-acid replacements are associated with adaptive changes in Rubisco

Post-cratering melting of target rocks at the impact melt contact: Observations from the Vredefort impact structure, South Africa

Impact melt is generated following hypervelocity impact events. Emplacement of impact melt dikes, such as the Vredefort Granophyre Dikes, allow for this high temperature melt to come into contact with deeply-buried target rocks after the cratering process is completed. Our study analyzes the effects of this interaction by examining the direct contact between the Vredefort Granophyre and the granitic host at the Kopjeskraal and Lesutoskraal Granophyre Dikes using scanning electron microscopy (SEM), energy-dispersive spectrometry (EDS), electron backscatter diffraction (EBSD), and X-ray micro-computed tomography (μCT). A several-mm-thick transition zone between the host rock and the impact melt is enriched in SiO2 and indicates preferential melting of feldspar and mica in the host rock by interaction with the impact melt. Immiscible droplets of newly-formed silicate melt migrated from the transition zone into the impact melt. We observe inundations of the impact melt along narrow fractures into the host rocks, which, in some cases, surround and incorporate fragments of the host rock into the melt body. We suggest three possible mechanisms by which components of the host rock can enter the impact melt: 1) fragmentation of the host rock prior to melt emplacement and subsequent entrainment into the melt; 2) inundations of melt around fragments of host rock at the contact, followed by incorporation of the host rock into the melt; 3) melting of the host rock and immiscible migration of melt fragments within the impact melt. The lack of observed assimilation of the granitic fragments into the impact melt, either because of silica saturation or viscosity contrast between the melts, suggests that the bulk composition of the Granophyre Dike matrix approximately represents the composition of the impact melt sheet.Copyright © 2024 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/). The linked file is the published version of the article.NHM Repositor

Prompt Neutrons from Spontaneous 254Rf Fission

The neutron yield from the spontaneous fission of the short-lived neutron-deficient 254Rf nucleus produced in the complete fusion reaction with the beam of multiply charged ions from the U400 accelerator at the Flerov Laboratory of Nuclear Reactions (FLNR), Joint Institute for Nuclear Research (JINR), is measured using the combined detection system of the SHELS velocity filter. The half-life and the branching ratio of 254Rf are also measured. The data on the multiplicity of prompt neutrons from spontaneous fission of 256Rf ($$\bar {\nu }$$ = 3.87 ± 0.34) are presented for the first time.The neutron yield from the spontaneous fission of the short-lived neutron-deficient 254Rf nucleus produced in the complete fusion reaction with the beam of multiply charged ions from the U400 accelerator at the Flerov Laboratory of Nuclear Reactions (FLNR), Joint Institute for Nuclear Research (JINR), is measured using the combined detection system of the SHELS velocity filter. The half-life and the branching ratio of 254Rf are also measured. The data on the multiplicity of prompt neutrons from spontaneous fission of 256Rf ($$\bar {\nu }$$ = 3.87 ± 0.34) are presented for the first time.status: publishe