Insights into methane dynamics from analysis of authigenic carbonates and chemosynthetic mussels at newly-discovered Atlantic Margin seeps

The recent discovery of active methane venting along the US northern and mid-Atlantic margin represents a new source of global methane not previously accounted for in carbon budgets from this region. However, uncertainty remains as to the origin and history of methane seepage along this tectonically inactive passive margin. Here we present the first isotopic analyses of authigenic carbonates and methanotrophic deep-sea mussels, Bathymodiolus sp., and the first direct constraints on the timing of past methane emission, based on samples collected at the upper slope Baltimore Canyon (∼385 m water depth) and deepwater Norfolk (∼1600 m) seep fields within the area of newly-discovered venting. The authigenic carbonates at both sites were dominated by aragonite, with an average image signature of image, a value consistent with microbially driven anaerobic oxidation of methane-rich fluids occurring at or near the sediment–water interface. Authigenic carbonate U and Sr isotope data further support the inference of carbonate precipitation from seawater-derived fluids rather than from formation fluids from deep aquifers. Carbonate stable and radiocarbon (image and image) isotope values from living Bathymodiolus sp. specimens are lighter than those of seawater dissolved inorganic carbon, highlighting the influence of fossil carbon from methane on carbonate precipitation. U–Th dates on authigenic carbonates suggest seepage at Baltimore Canyon between image to image, and at the Norfolk seep field between image to image, providing constraint on the longevity of methane efflux at these sites. The age of the brecciated authigenic carbonates and the occurrence of pockmarks at the Baltimore Canyon upper slope could suggest a link between sediment delivery during Pleistocene sea-level lowstand, accumulation of pore fluid overpressure from sediment compaction, and release of overpressure through subsequent venting. Calculations show that the Baltimore Canyon site probably has not been within the gas hydrate stability zone (GHSZ) in the past 20 ka, meaning that in-situ release of methane from dissociating gas hydrate cannot be sustaining the seep. We cannot rule out updip migration of methane from dissociation of gas hydrate that occurs farther down the slope as a source of the venting at Baltimore Canyon, but consider that the history of rapid sediment accumulation and overpressure may play a more important role in methane emissions at this site

Seasonal variability in the source and composition of particulate matter in the depositional zone of Baltimore Canyon, U.S. Mid-Atlantic Bight

Submarine canyons are often hotspots of biomass due to enhanced productivity and funneling of organic matterof marine and terrestrial origin. However, most deep-sea canyons remain poorly studied in terms of their role asconduits of terrestrial and marine particles. A multi-tracer geochemical investigation of particles collectedyearlong by a sediment trap in Baltimore Canyon on the US Mid-Atlantic Bight (MAB) revealed temporalvariability in source, transport, and fate of particulate matter. Both organic biomarker composition (sterol and nalkanes)and bulk characteristics (δ13C, Δ14C, Chl-a) suggest that while on average the annual contribution ofterrestrial and marine organic matter sources are similar, 42% and 52% respectively, marine sources dominate.Elevated Chlorophyll-a and sterol concentrations during the spring sampling period highlight a seasonal influx ofrelatively fresh phytodetritus. In addition, the contemporaneous increase in the particle reactive micronutrientscadmium (Cd) and molybdenum (Mo) in the spring suggest increased scavenging, aggregation, and sinking ofphytodetrital biomass in response to enhanced surface production within the nutricline. While tidally drivencurrents within the canyon resuspend sediment between 200 and 600 m, resulting in the formation of a nepheloidlayer rich in lithogenic material, near-bed sediment remobilization in the canyon depositional zone wasminimal. Instead, vertical transport and lateral transport across the continental margin were the dominantprocesses driving seasonal input of particulate matter. In turn, seasonal variability in deposited particulate organicmatter is likely linked to benthic faunal composition and ecosystem scale carbon cycling

Hydrothermal vents and methane seeps: rethinking the sphere of influence

Although initially viewed as oases within a barren deep ocean, hydrothermal vents and methane seep chemosynthetic communities are now recognized to interact with surrounding ecosystems on the sea floor and in the water column, and to affect global geochemical cycles. The importance of understanding these interactions is growing as the potential rises for disturbance of the systems from oil and gas extraction, seabed mining and bottom trawling. Here we synthesize current knowledge of the nature, extent and time and space scales of vent and seep interactions with background systems. We document an expanded footprint beyond the site of local venting or seepage with respect to elemental cycling and energy flux, habitat use, trophic interactions, and connectivity. Heat and energy are released, global biogeochemical and elemental cycles are modified, and particulates are transported widely in plumes. Hard and biotic substrates produced at vents and seeps are used by "benthic background" fauna for attachment substrata, shelter, and access to food via grazing or through position in the current, while particulates and fluid fluxes modify planktonic microbial communities. Chemosynthetic production provides nutrition to a host of benthic and planktonic heterotrophic background species through multiple horizontal and vertical transfer pathways assisted by flow, gamete release, animal movements, and succession, but these pathways remain poorly known. Shared species, genera and families indicate that ecological and evolutionary connectivity exists among vents, seeps, organic falls and background communities in the deep sea: the genetic linkages with inactive vents and seeps and background assemblages however, are practically unstudied. The waning of venting or seepage activity generates major transitions in space and time that create links to surrounding ecosystems, often with identifiable ecotones or successional stages. The nature of all these interactions is dependent on water depth, as well as regional oceanography and biodiversity. Many ecosystem services are associated with the interactions and transitions between chemosynthetic and background ecosystems, for example carbon cycling and sequestration, fisheries production, and a host of non-market and cultural services. The quantification of the sphere of influence of vents and seeps could be beneficial to better management of deep-sea environments in the face of growing industrialization

Food-web dynamics and isotopic niches in deep-sea communities residing in a submarine canyon and on the adjacent open slopes

Examination of food webs and trophic niches provide insights into organisms’ functionalecology, yet few studies have examined trophodynamics within submarine canyons, wherethe interaction of canyon morphology and oceanography influences habitat provision and fooddeposition. Using stable isotope analysis and Bayesian ellipses, we documented deep-sea foodwebstructure and trophic niches in Baltimore Canyon and the adjacent open slopes in the USMid-Atlantic Region. Results revealed isotopically diverse feeding groups, comprising approximately5 trophic levels. Regression analysis indicated that consumer isotope data are structuredby habitat (canyon vs. slope), feeding group, and depth. Benthic feeders were enriched in 13C and15N relative to suspension feeders, consistent with consuming older, more refractory organic matter.In contrast, canyon suspension feeders had the largest and more distinct isotopic niche, indicatingthey consume an isotopically discrete food source, possibly fresher organic material. Thewider isotopic niche observed for canyon consumers indicated the presence of feeding specialistsand generalists. High dispersion in δ13C values for canyon consumers suggests that the isotopiccomposition of particulate organic matter changes, which is linked to depositional dynamics,resulting in discrete zones of organic matter accumulation or resuspension. Heterogeneity in habitatand food availability likely enhances trophic diversity in canyons. Given their abundance in theworld’s oceans, our results from Baltimore Canyon suggest that submarine canyons may representimportant havens for trophic diversity

On the Efficiency of Sukhatme\u27s Test

Deep-sea ecosystems encompass unique and often fragile communities that are sensitive to a variety of anthropogenic and natural impacts. After the 2010 Deepwater Horizon (DWH) oil spill, sampling efforts documented the acute impact of the spill on some deep-sea coral colonies. To investigate the impact of the DWH spill on quality and quantity of biomass delivered to the deep-sea, a suite of geochemical tracers (e.g., stable and radio-isotopes, lipid biomarkers, and compound-specific isotopes) was measured from monthly sediment trap samples deployed near a high-density deep-coral site in the Viosca Knoll area of the north–central Gulf of Mexico prior to (Oct-2008 to Sept-2009) and after the spill (Oct-10 to Sept-11). Marine (e.g., autochthonous) sources of organic matter (OM) dominated the sediment traps in both years, however after the spill, there was a pronounced reduction in marine-sourced OM, including a reduction in marine-sourced sterols and n-alkanes and a concomitant decrease in sediment trap organic carbon and pigment flux. Results from this study indicate a reduction in primary production and carbon export to the deep-sea in 2010–2011, at least 6–18 months after the spill started. Whereas satellite observations indicate an initial increase in phytoplankton biomass, results from this sediment trap study define a reduction in primary production and carbon export to the deep-sea community. In addition, a dilution from a low-14C carbon source (e.g., petro-carbon) was detected in the sediment trap samples after the spill, in conjunction with a change in the petrogenic composition. The data presented here fills a critical gap in our knowledge of biogeochemical processes and sub-acute impacts to the deep-sea that ensued after the 2010 DWH spill

Impacts of exotic mangrove forests and mangrove deforestation on carbon remineralization and ecosystem functioning in marine sediments

To evaluate how mangrove invasion and removal can modify short-term benthic carbon cycling and ecosystem functioning, we used stable-isotopically labeled algae as a deliberate tracer to quantify benthic respiration and C-flow over 48 h through macrofauna and bacteria in sediments collected from (1) an invasive mangrove forest, (2) deforested mangrove sites 2 and 6 years after removal of above-sediment mangrove biomass, and (3) two mangrove-free control sites in the Hawaiian coastal zone. Sediment oxygen consumption (SOC) rates averaged over each 48 h investigation were significantly greater in the mangrove and mangrove removal site experiments than in controls and were significantly correlated with total benthic (macrofauna and bacteria) biomass and sedimentary mangrove biomass (SMB). Bacteria dominated short-term C-processing of added microalgal-C and benthic biomass in sediments from the invasive mangrove forest habitat and in the 6-yr removal site. In contrast, macrofauna were the most important agents in the short-term processing of microalgal-C in sediments from the 2-yr mangrove removal site and control sites. However, mean faunal abundance and C-uptake rates in sediments from both removal sites were significantly higher than in control cores, which collectively suggest that community structure and short-term C-cycling dynamics of sediments in habitats where mangroves have been cleared can remain fundamentally different from un-invaded mudflat sediments for at least 6-yrs following above-sediment mangrove removal. In summary, invasion by mangroves can lead to dramatic shifts in benthic ecosystem function, with sediment metabolism, benthic community structure and short-term C-remineralization dynamics being affected for years following invader removal.