CD1a+ and CD207+ cells are reduced in oral submucous fibrosis and oral squamous cell carcinoma

The objective of this study investigated the distribution of immature dendritic cells (DCs), Langerhans cells and plasmacytoid DCs in oral submucous fibrosis (OSMF), OSMF associated with oral squamous cell carcinoma (OSMF-OSCC), oral leukoplakia (OL), and oral squamous cell carcinoma (OSCC). Fourteen cases of OSMF, 9 of OSMF-OSCC, 8 of OL¸ 45 of OSCC and 8 of normal epithelium were retrospectively retrieved and their diagnoses confirmed. Immunoreactions against CD1a, CD207 e CD303 were performed and the number of positive cells quantified. A significant decrease of CD1a+ was found in OSMF (p?0.05), OSMF-OSCC (p ? 0.01), and OSCC (p ? 0.001) when compared to normal epithelium. For CD207+ the significance decrease was observed in OSMF-OSCC (p ? 0.05), and OSCC (p ? 0.01) when compared with normal epithelium, and in OSMF when compared with OL (p ? 0.05). There was no significant difference for CD303, but increased in CD303+ was observed in OSCC when compared with normal epithelium. The decrease in the number of CD1a+ and CD207+ cells may be associate to the development of oral OSCC, and in OPMDs they might be indicators of malignant transformation

Pilates training improves 5-km run performance by changing metabolic cost and muscle activity in trained runners (vol 13, e0194057, 2018)

Dr. Jorge L.L. Storniolo should be included in the author byline. He should be listed as fifth author, and his affiliation is 3: Laboratory of Locomotion Physiomechanics, Department of Pathophysiology and Transplantation, University of Milan, Italy. The contributions of this author are as follows: Conceptualization, Investigation, Methodology, Writing\u2013Review & Editing. The correct citation is: Finatto P, Silva ESD, Okamura AB, Almada BP, Storniolo JLL, Oliveira HB (2018) Pilates training improves 5-km run performance by changing metabolic cost and muscle activity in trained runners. PLoS ONE 13(3): e0194057. https://doi.org/10.1371/journal.pone.019405

Malária entre populações indígenas do Brasil

No presente trabalho a Autora descreve a situação da malária entre populações indígenas do Brasil, desde a época colonial até o momento presente, enfatizando a falta de informações científicas sobre assunto de tanta relevância para o país.<br>In the current paper the Author describes the situation of malaria among the aboriginal natives of Brazil since the colonial times. Attention is called to the scanty scientific information on a subject of so much relevance for this country

Effect of the extract of Ricinus communis L. on the osmotic fragility, labeling of red blood cells with Technetium-99m and morphology of the cells

The aim of this study was to evaluate the influence of the proteic extract of R. communis on the cell physiology by the osmotic fragility, labeling of the blood elements with the 99mTc and cell morphology. To evaluate the osmotic fragility, the blood samples of the Wistar rats were incubated with the concentrations of R. communis and with the solutions of NaCl (0.4; 0.7; 0.9%). In the labeling of the blood elements procedure, the rat blood was treated with a solution of Tc-99m and TCA at 5%, determining the rate of radioactivity (%ATI) in the plasma (P) and in the red blood cells (RBC). The soluble and insoluble fractions of the plasma were also evaluated. The cells morphology submitted to the extract was evaluated by the optical microscopy (x40). The results indicated that the rate of the hemolysis increased in the presence of 0.125 mg/mL of the extract. There was a decay of 49.69% in the rate of ATI in the insoluble fraction of the cells, with the morphological alterations in the red blood cells. These results suggested that the extract changed the capability of binding of the red blood cells due to the stannous ion oxidation, modifying the cells structure.<br>Produtos naturais são usados freqüentemente por muitas pessoas no tratamento do câncer. O Ricinus communis L é uma Euforbiaceae que apresenta propriedades laxativas, purgativas e antitumorais. O objetivo deste trabalho é estudar a influência da fração protéica do extrato hidroalcoólico de R. communis L. na fisiologia celular através da fragilidade osmótica, da marcação de elementos sanguíneo com 99mTc e da morfologia celular. Para avaliar a fragilidade osmótica, amostras de sangue de ratos Wistar foram incubadas com concentrações de R. communis e com soluções de NaCl (0,4; 0,7; 0,9%). No procedimento de marcação de elementos sanguíneos, as amostras de sangue foram tratadas com solução de Tc-99m e TCA à 5%, determinando o percentual de radioatividade (%ATI) no plasma (P) e nas células vermelhas (RBC); as frações solúvel e insolúvel do plasma também foram avaliadas. A morfologia das células submetidas ao extrato foi avaliada por microscopia óptica (x40). Os resultados indicam que o extrato na concentração de 0,125 mg/mL provoca hemólise de 49,69% , no % ATI na fração insolúvel das células, ocorrendo alterações morfológicas das células sangüíneas. Esses resultados sugerem que o extrato radiomodifica a ligação do 99mTc às células vermelhas. Isto pode ser devido à oxidação do íon estanoso, a um processo de competição com os sítios de ligação do 99mTc ou por modificação das estruturas da membrana