Replication of Influenza A and B Viruses in Human Diploid Cells

Artículo científico -- Universidad de Costa Rica, Instituto de Investigaciones en Salud.1983Under optimal conditions, of high multiplicities of infection and with trypsin included in the medium throughout the incubation period, high yields of infectious influenza A and B viruses (106-5 p.f.u./ml) and of antigenically active haemagglutinin (HA) (1 ug/HA/106 cells) were produced in human diploid MRC-5 cells. Budding virus particles were seen as spherical or short rod-like protrusions on the surface of the infected cells, and also on cell filopodia. Virus-induced cytoplasmic and nuclear inclusions were present in infected cells. This virus—human cell system may be suitable for studies of influenza virus persistence and for production of immunologically active HA antigen.Universidad de Costa Rica, Instituto de Investigaciones en SaludUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto de Investigaciones en Salud (INISA

A protocol for developing a complex needs indicator for veterans (CNIV) in the UK

Introduction: The veteran population in the UK has been decreasing, however, there remains a proportion of veterans and their families who continue to experience multiple and complex health, financial, and social needs. The complex problems tend to exacerbate each other and deepen over time if appropriate support is not provided. Identifying the veterans with complex needs is crucial for effective support by military charities and health and social care services. The present research aims to develop a complex needs indicator for the veteran population (CNIV) that will quantify complexity and help to identify the risk of having or developing complex needs. Methods: The development of the CNIV will be informed by the guidance for constructing composite indicators. The data on grant support received by veterans’ beneficiaries from the UK Royal Marine and SSFA charities will be used for designing the indicator and evaluating its robustness. The crucial step in constructing the indicator is assigning weights to different needs and risk factors associated with complex cases. Factor analysis (FA) and analytical network process (ANP) will be used as weighting methods for the analysed variables. Conclusion: The development of CNIV has important implications for research and practice, such as the potential to be used as a screening tool for identifying complex cases, improved provision of the targeted support to veterans, assessing the scope of complex problems among veterans within the country and informing policy makers and a more general audience of the complexity of need within the sector

Flavour and Collider Interplay for SUSY at LHC7

The current 7 TeV run of the LHC experiment shall be able to probe gluino and squark masses up to values larger than 1 TeV. Assuming that hints for SUSY are found in the jets plus missing energy channel by the end of a 5 fb$^{-1}$ run, we explore the flavour constraints on three models with a CMSSM-like spectrum: the CMSSM itself, a Seesaw extension of the CMSSM, and Flavoured CMSSM. In particular, we focus on decays that might have been measured by the time the run is concluded, such as $B_s\to\mu\mu$ and $\mu\to e\gamma$. We also analyse constraints imposed by neutral meson bounds and electric dipole moments. The interplay between collider and flavour experiments is explored through the use of three benchmark scenarios, finding the flavour feedback useful in order to determine the model parameters and to test the consistency of the different models.Comment: 44 pages, 15 figures; v3: minor corrections, added references, updated figures. Version accepted for publicatio

Constituent Quark Model Calculation for a possible J^P=0^-,T=0 Dibaryon

There exists experimental evidence that a dibaryon resonance d' with quantum numbers J^P=0^-,T=0 and mass 2065 MeV could be the origin of the narrow peak in the (\pi^+ ,\pi^- ) double charge exchange cross--sections on nuclei. We investigate the six--quark system with these quantum--numbers within the constituent quark model, with linear confinement, effective one--gluon exchange at short range and chiral interactions between quarks (\pi and \sigma exchange). We classify all possible six quark states with J^P=0^-,T=0, and with N=1 and N=3 harmonic oscillator excitations, using different reduction chains. The six--quark Hamiltonian is diagonalized in the basis including the unique N=1 state and the 10 most important states from the N=3 shell. We find, that with most of the possible sets of parameters, the mass of such a "dibaryon" lies above the N(939)+N^\ast(1535) threshold. The only possibility to describe the supposed d'(2065) in the present context is to reduce the confinement strength to very small values, however at the expense of describing the negative parity resonances N^\ast. We also analyze the J^P=0^-,T=2,N=1 six--quark state.Comment: 42 pages, Latex, submitted to Nucl.Phys.

Quaternion-Octonion Unitary Symmetries and Analogous Casimir Operators

An attempt has been made to investigate the global SU(2) and SU(3) unitary flavor symmetries systematically in terms of quaternion and octonion respectively. It is shown that these symmetries are suitably handled with quaternions and octonions in order to obtain their generators, commutation rules and symmetry properties. Accordingly, Casimir operators for SU(2)and SU(3) flavor symmetries are also constructed for the proper testing of these symmetries in terms of quaternions and octonions

Nutty Bubbles

We investigate the various time-dependent bubble spacetimes that can be obtained from double analytic continuation of asymptotically locally flat/AdS spacetimes with NUT charge. We find different time-dependent explicit solutions of general relativity from double analytic continuations of Taub-Nut(-AdS) and Kerr-Nut(-AdS) spacetimes. One solution in particular has Milne-like evolution throughout, and another is a NUT-charged generalization of the AdS soliton. These solutions are all four dimensional. In certain situations the NUT charge induces an ergoregion into the bubble spacetime and in other situations it quantitatively modifies the evolution of the bubble, as when rotation is present. In dimensions greater than four, no consistent bubble solutions are found that have only one timelike direction.Comment: 29 pages, 10 figure

Rotating Resonator-Oscillator Experiments to Test Lorentz Invariance in Electrodynamics

In this work we outline the two most commonly used test theories (RMS and SME) for testing Local Lorentz Invariance (LLI) of the photon. Then we develop the general framework of applying these test theories to resonator experiments with an emphasis on rotating experiments in the laboratory. We compare the inherent sensitivity factors of common experiments and propose some new configurations. Finally we apply the test theories to the rotating cryogenic experiment at the University of Western Australia, which recently set new limits in both the RMS and SME frameworks [hep-ph/0506074].Comment: Submitted to Lecture Notes in Physics, 36 pages, minor modifications, updated list of reference

Out of equilibrium: understanding cosmological evolution to lower-entropy states

Despite the importance of the Second Law of Thermodynamics, it is not absolute. Statistical mechanics implies that, given sufficient time, systems near equilibrium will spontaneously fluctuate into lower-entropy states, locally reversing the thermodynamic arrow of time. We study the time development of such fluctuations, especially the very large fluctuations relevant to cosmology. Under fairly general assumptions, the most likely history of a fluctuation out of equilibrium is simply the CPT conjugate of the most likely way a system relaxes back to equilibrium. We use this idea to elucidate the spacetime structure of various fluctuations in (stable and metastable) de Sitter space and thermal anti-de Sitter space.Comment: 27 pages, 11 figure

Flavour SU(3) Symmetry in Charmless B Decays

QCD sum rules are used to estimate the flavour SU(3)-symmetry violation in two-body B decays to pions and kaons. In the factorizable amplitudes the SU(3)-violation manifests itself in the ratio of the decay constants f_K/f_pi and in the differences between the B->K, B_s->K and B->pi form factors. These effects are calculated from the QCD two-point and light-cone sum rules, respectively, in terms of the strange quark mass and the ratio of the strange and nonstrange quark-condensate densities. Importantly, QCD sum rules predict that SU(3) breaking in the heavy-to-light form factors can be substantial and does not vanish in the heavy-quark mass limit. Furthermore, we investigate the strange-quark mass dependence of nonfactorizable effects in the B->K pi decay amplitudes. Taking into account these effects we estimate the accuracy of several SU(3)-symmetry relations between charmless B-decay amplitudes.Comment: Two references added, version to be published in Phys.Rev.D, 21 pages, 12 postscript figure