Eficiência do protocolo Ovsynch em ovelhas da raça Santa Inês

Twenty six Santa Inês ewes were asigned to three treatments to evaluate the efficiency of the Ovsynch protocol. In the treatment 1 - control (n= 8), the estrus was synchronized with sponges containing 60 mg MAP for 14 days. On D14, 300 IU eCG were administered. In treatment 2 (n= 9) the Ovsynch protocol was used: 25 µg of GnRH (D0), 37.5 µg of PGF2a (D7) and 25 µg of GnRH (D9). In treatment 3 (n= 9) the modified Ovsynch protocol was used: the administration of PGF2a and second GnRH as two days early. Estrus detection was accomplished using teaser. All ewes were mated twice with 12 hours of interval. Pregnancy rate (PR) was evaluated by ultrasonography 30 days after the end of mating. Estrus response was of 88.46% on average, and without differences among treatments (p>0.05). The interval for onset of estrus was greater (p0.05). Pregnancy rate was significantly greater (p0,05). O intervalo entre o final do tratamento e o início do estro foi maior (p0,05) entre tratamentos. A TP diferiu entre os grupos (37,5%, 62,5% e 25%, respectivamente. p<0,05). Os resultados mostram eficiência superior do protocolo Ovsynch, sob as restantes condições expe-rimentais

Synthesis and magnetic interaction on concentrated Fe3O4 nanoparticles obtained by the co-precipitation and hydrothermal chemical methods

In this work, a comparative study of the synthesis and magnetic properties of Fe3O4 nanoparticles obtained by the co-precipitation and hydrothermal methods with the adding of the sucrose as a chelating agent is reported. The analysis of transmission electron microscopy (TEM) show that particles are spherical-like with an average particle size ranging of 3 ≤d ≤ 10 nm. Magnetization measurements as a function of an applied magnetic field and temperature are consistent with a superparamagnetic behavior with small TB values. The analysis of the MvsH loops measured at T = 2 K and Zero-Field-Cooled and Field-Cooled (ZFC−FC) curves allows us to estimate the average diameter of Fe3O4 nanoparticles. These results are in good agreement with those obtained through X-ray diffraction (XRD) data (by using Scherrer equation and Williamson-Hall plot) and slightly smaller than those estimated using TEM images. Finally, ZFC−FC data are also used to evaluate the effective anisotropy constants, which are similar to those values found in literature considering the average sizes values estimated by different techniques468Part A1114911115

Antinociceptive Activity Of Ipomoea Imperati (vahl) Griseb., Convolvulaceae [atividade Antinociceptiva De Ipomoea Imperati (vahl) Griseb., Convolvulaceae]

Ipomoea imperati (Vahl) Griseb., Convolvulaceae, is used in folk medicine for the treatment of inflammation, swelling and wounds, as well as to treat pains after childbirth and for stomach problems. Administration of ethanol extract, lipid and aqueous fraction of I. imperati (300, 100 and 200 mg/kg) significantly inhibited the abdominal constriction in mice induced by acetic acid; increased the sleeping time evoked by pentobarbital sodium and showed a significant activity by inhibiting formalin-induced paw edema in mice. The same dose of I. imperati also raised the pain of mice in the hot-plate test and increased the latency at all observation times. The pre-treatment of the animals with naloxone (5 mg/kg, i.p.) suggested the participation of the opioid system in the antinociceptive effect of Ipomoea imperati.202180185Amanlou, M., Dadkhah, F., Salehnia, A., Farsam, H., An anti-inflammatory and anti-nociceptive effects of hydroalcoholic extract of Satureja khuzistanica Jamzad extract (2005) J Pharm Pharm Sci, 8, pp. 102-106Austin, D.F., Flora of Panama (1975) Ann Mo Bot Gard, 62, pp. 198-201Collier, H.O., Dinneen, L.C., Johnson, C.A., Schneider, C., The abdominal constriction response and its suppression by analgesic drugs in the mouse (1968) Brit J Pharmacol Chemother, 32, pp. 295-310Costa-Lotufo, L.V., de Lucena, D.F., Andrade Neto, M., Bezerra, J.N.S., Leal, L.K.A.M., de Sousa, F.C.F., Viana, G.S., Analgesic, anti-inflammatory and central depressor effects of the hydroalcoholic extract and fractions from Aeolanthus suaveolens (2004) Biol Pharm Bull, 27, pp. 821-824di Rosa, M., Giroud, J.P., Willoughby, D.A., Studies on the mediators of the acute inflammatory response induced in rats in different sites by carrageenan and turpentine (1971) J Pathol, 104, pp. 15-29Duarte, I.D.G., Nakamura, M., Ferreira, S.H., Participation of the sympathetic system in acetic acid-induced writhing in mice (1988) Braz J Med Biol Res, 21, pp. 341-343Eddy, N.B., Leimback, D.J., Synthetic analgesics. II. Dithienylbutenyl and dithienylbutylamines (1953) J Pharmacol Exp Ther, 107, pp. 385-393Ferreira, S.H., Moncada, S., Vane, J.R., The blockade of the local generation of prostaglandins explains the analgesic action of aspirin (1973) Agents Actions, 3, p. 385Ferreira, A.A., Amaral, F.A., Duarte, I.D.G., Oliveira, P.M., Alves, R.B., Silveira, D., Azevedo, A.O., Castro, M.S.A., Antinociceptive effect from Ipomoea cairica extract (2006) J Ethnopharmacol, 105, pp. 148-153Fosberg, F.R., Sachet, M.H., Flora of Micronesia. 3. Convolvulaceae (1977) Contrib Bot, 36, pp. 1-34Gracioso, J.S., Paulo, M.Q., Hiruma-Lima, C.A., Souza Brito, A.R.M., Antinociceptive effect in mice of a hydroalcoholic extract of Neurolaena lobata (L.) R. Br. and its organic fractions (1998) J Pharma Pharmacol, 50, pp. 1425-1429Henriques, M.G.M.O., Silva, P.M.R., Martins, M.A., Flores, C.A., Cunha, F.Q., Assreuy-Filho, J., Cordeiro, R.S.B., Mouse paw edema. A new model for inflammation (1987) Braz J Med Biol Res, 20, pp. 243-249Hiruma-Lima, C.A., Gracioso, J.S., Bighetti, E.J.B., Germonsén Robineou, L., Souza Brito, A.R.M., The juice of fresh leaves of Boerhaavia diffusa L. (Nyctaginaceae) markedly reduces pain in mice (2000) J Ethnopharmacol, 71, pp. 267-274Jansen, P.A.J., Niememegeers, C.J.E., Dony, G.H., The inhibitory effect of fentanyl and other morphine-like analgesics on the warm water induced tail withdrawl reflex in rats (1963) Arzneimittel-Forsch, 13, pp. 502-507Koster, R., Anderson, M., de Beer, E.J., Acetic acid for analgesic screening (1959) Fed Proc, 18, pp. 412-418le Bars, D., Gozariu, M., Cadden, S.W., Animal models of nociception (2001) Pharmacol Rev, 53, pp. 597-652Ma, Y., Han, H., Eun, J.S., Kim, H.C., Hong, J.T., Oh, K.W., Sanjoinine A isolated from Zizyphi Spinosi Semen augments pentobarbital-induced sleeping behaviors through the modification of Gabaergic system (2007) Biol Pharm Bull, 30, pp. 1748-1753Macleod, J.K., Ward, A., Oelrichs, P.B., Structural investigation of resin glycosides from Ipomoea lonchophylla (1997) J Nat Prod, 60, pp. 467-471Noda, N., Takahashi, N., Kawasaki, T., Miyahara, K., Yang, C.R., Stoloniferins I-VII, resin glycosides from Ipomoea stolonifera (1994) Phytochemistry, 36, pp. 365-371Olfert, E.D., Cross, B.M., McWillian, A.A., (1993) Guide to The Care and Use of Experimental Animals, pp. 1-213. , Canadian council on animal care co., OntarioPan, Y.X., Xu, J., Bolan, E., Abbadie, C., Chang, A., Zuckeman, A., Rossi, G., Pasternak, G.W., Identification and characterization of three new alternatively spliced muopioid receptor isoforms (1999) Mol Pharmacol, 56, pp. 396-403Paula, A.C.B., Hayashi, L.S.S., Freitas, J.C., Anti-inflammatory and antispasmodic activity of Ipomoea imperati (Vahl) Griseb (Convolvulaceae) (2003) Braz J Med Biol Res, 36, pp. 105-112Pieretti, S., di Giannuario, A., Loizzo, A., Sagratella, S., Scotti de Carolis, A., Capasso, A., Sorrentino, L., Dexamethasone prevents epileptiform activity induced by morphine in vivo and in vitro experiments (1992) J Pharmacol Exp Ther, 263, pp. 830-839Pongprayoon, U., Bohlin, L., Soonthornsaratune, P., Wasuwat, S., Anti-inflammatory activity of Ipomoea pescaprae (L.) R (1991) Br. Phytother Res, 5, pp. 63-66Pongprayoon, U., Baeckstrom, P., Jacobsson, U., Lindstrom, M., Bohlin, L., Compounds inhibiting prostaglandin synthesis isolated from Ipomoea pes caprae (1991) Planta Med, 57, pp. 515-517Pongprayoon, U., Baeckstrom, P., Jacobsson, U., Lindstrom, M., Bohlin, L., Antispasmodic activity of beta-damascenone and E-phytol isolated from Ipomoea pes-caprae (1992) Planta Med, 58, pp. 19-21Shibata, M., Ohkubo, T., Takahashi Hiroki, R., Modified formalin test: Characteristic biphasic pain response (1989) Pain, 38, pp. 347-352Souza, M.M., Madeira, A., Berti, C., Krogh, R., Yunes, R.A., Cechinel, V.J., Antinociceptive properties of the methanolic extract obtained from Ipomoea pes-caprae (L.) R (2000) Br. J Ethnopharmacol, 69, pp. 85-90Trentin, A.P., Santos, A.R.S., Miguel, O.G., Pizzolatti, M.G., Yunes, R.A., Calixto, J.B., Mechanisms involved in the antinociceptive effect in mice of the hydroalcoholic extract of Siphocampylus verticillatus (1997) J Pharm Pharmacol, 49, pp. 567-572Woolfe, G., Macdonald, A.D., The evaluation of the analgesic action of pethidine hydrochloride (demerol) (1944) J Pharmacol Exp Ther, 80, pp. 300-307Zimmerman, M., Ethical guidelines for investigations of experimental pain in conscious animals (1983) Pain, 16, pp. 109-11

Efeito dos ácidos graxos essenciais sobre lipidemia e vascularização da membrana vitelina de codornas japonesas

Verificou-se o efeito de diferentes fontes de ácidos graxos essenciais ômega-3 e ômega-6 sobre o perfil lipídico de codornas japonesas e sobre o crescimento vascular na membrana vitelina dos embriões de aves suplementadas com óleo de peixe, 2% e 4%, e óleo de soja, 2% e 4%, em relação à dieta-controle, sem suplementação lipídica. Foi usado o método enzimático com reação colorimétrica para estimar o perfil lipídico sérico de colesterol total, triglicerídeos e HDL das aves. A vascularização na membrana vitelina foi quantificada por meio da dimensão fractal, utilizando-se o método de box-counting. A concentração de colesterol dos grupos controle e tratados não diferiu entre si. Para o HDL, o grupo que recebeu maior proporção de óleo de peixe, 4%, diferiu dos outros grupos. Os níveis de triglicerídeos das codornas suplementadas com óleo de peixe foram superiores aos dos demais grupos. A dimensão fractal da vascularização da membrana vitelina dos embriões de codornas dos grupos que receberam 4% de óleo de peixe e 4% de óleo de soja foi significativamente menor que a dos demais grupos, indicando efeitos antiangiogênicos no processo de formação vascular