5 research outputs found
Lithology and geochemistry of Albian-Cenomanian sandy sequences of the Voronezh Anteclise and their paleogeographic implication
Quelles sont les autres causes que l’asphyxie périnatale pour expliquer les infirmités motrices d’origine cérébrale?
Sphingosine kinase interacts with TRAF2 and dissects tumor necrosis factor-a signaling
Tumor necrosis factor-alpha (TNF) receptor-associated factor 2 (TRAF2) is one of the major mediators of TNF receptor superfamily transducing TNF signaling to various functional targets, including activation of NF-kappa B, JNK, and antiapoptosis. We investigated how TRAF2 mediates differentially the distinct downstream signals. We now report a novel mechanism of TRAF2-mediated signal transduction revealed by an association of TRAF2 with sphingosine kinase (SphK), a lipid kinase that is responsible for the production of sphingosine 1-phosphate. We identified a TRAF2-binding motif of SphK that mediated the interaction between TRAF2 and SphK resulting in the activation of the enzyme, which in turn is required for TRAF2-mediated activation of NF-kappa B but not JNK. In addition, by using a kinase inactive dominant-negative SphK and a mutant SphK that lacks TRAF2-binding motif we show that the interaction of TRAF2 with SphK and subsequent activation of SphK are critical for prevention of apoptosis during TNF stimulation. These findings show a role for SphK in the signal transduction by TRAF2 specifically leading to activation of NF-kappa B and antiapoptosis.Pu Xia, Lijun Wang, Paul A. B. Moretti, Nathaniel Albanese, Fugui Chai, Stuart M. Pitson, Richard J. D'Andrea, Jennifer R. Gamble and Mathew A. Vada